Presence of circulating macromolecular IgA in patients with hematuria due to primary IgA nephropathy
The relation between renal histologic features and the presence of circulating immune complexes was studied in 50 patients with hematuria. Primary IgA nephropathy was found in 25 patients, and various other forms of glomerulopathy were seen in the remaining 25 patients. Circulating immune complexes...
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| Veröffentlicht in: | Am. J. Med.; (United States) 1983-03, Vol.74 (3), p.375-381 |
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| creator | Valentijn, Robert M. Kauffmann, Robert H. De La Rivière, Guy Brutel Daha, Mohamed R. Van Es, Leendert A. |
| description | The relation between renal histologic features and the presence of circulating immune complexes was studied in 50 patients with hematuria. Primary IgA nephropathy was found in 25 patients, and various other forms of glomerulopathy were seen in the remaining 25 patients. Circulating immune complexes were detected with the
125I-C1q-binding assay, the conglutinin-binding assay, and the anti-IgA inhibition binding assay, the latter detecting specifically IgA-containing immune complex-like material. The
125I-C1q-binding assay gave negative findings for all patients except one. With the conglutinin-binding assay, immune complexes were found in a similar frequency for patients with and without IgA nephropathy. However, the anti-IgA inhibition binding assay gave positive results only in patients with primary IgA nephropathy (68 percent) and in none of the other patients. Sucrose density ultracentrifugation, as well as experiments in which the anti-IgA inhibition binding assay was performed with and without pretreatment of serum with polyethylene glycol, showed the presumed IgA immune complexes to have intermediate sedimentation coefficients (11 to 21S). The presence and level of this macromolecular IgA in the circulation correlated significantly (p < 0.001) with the presence of hematuria in patients who had this clinical manifestation intermittently. Furthermore, a significant correlation (r = 0.69, p < 0.0001) was found between the degree of hematuria and the degree of positive findings of the anti-IgA inhibition binding assay. This study shows that in patients presenting with hematuria, a positive finding on the anti-IgA inhibition binding assay is restricted to patients with primary IgA nephropathy and therefore could be of diagnostic value. |
| doi_str_mv | 10.1016/0002-9343(83)90954-3 |
| format | Article |
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125I-C1q-binding assay, the conglutinin-binding assay, and the anti-IgA inhibition binding assay, the latter detecting specifically IgA-containing immune complex-like material. The
125I-C1q-binding assay gave negative findings for all patients except one. With the conglutinin-binding assay, immune complexes were found in a similar frequency for patients with and without IgA nephropathy. However, the anti-IgA inhibition binding assay gave positive results only in patients with primary IgA nephropathy (68 percent) and in none of the other patients. Sucrose density ultracentrifugation, as well as experiments in which the anti-IgA inhibition binding assay was performed with and without pretreatment of serum with polyethylene glycol, showed the presumed IgA immune complexes to have intermediate sedimentation coefficients (11 to 21S). The presence and level of this macromolecular IgA in the circulation correlated significantly (p < 0.001) with the presence of hematuria in patients who had this clinical manifestation intermittently. Furthermore, a significant correlation (r = 0.69, p < 0.0001) was found between the degree of hematuria and the degree of positive findings of the anti-IgA inhibition binding assay. This study shows that in patients presenting with hematuria, a positive finding on the anti-IgA inhibition binding assay is restricted to patients with primary IgA nephropathy and therefore could be of diagnostic value.</description><identifier>ISSN: 0002-9343</identifier><identifier>EISSN: 1555-7162</identifier><identifier>DOI: 10.1016/0002-9343(83)90954-3</identifier><identifier>PMID: 6219575</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics ; BETA DECAY RADIOISOTOPES ; Binding Sites, Antibody ; BIOLOGICAL MATERIALS ; BIOLOGICAL WASTES ; BLOOD SERUM ; BODY ; BODY FLUIDS ; Centrifugation, Density Gradient ; Complement Activating Enzymes - analysis ; Complement C1q ; Complement Fixation Tests ; DAYS LIVING RADIOISOTOPES ; DISEASES ; ELECTRON CAPTURE RADIOISOTOPES ; GLOBULINS ; Glomerulonephritis - immunology ; Hematuria - etiology ; Hematuria - immunology ; Humans ; Immune Complex Diseases - complications ; Immunoglobulin A - analysis ; IMMUNOGLOBULINS ; INHIBITION ; INTERMEDIATE MASS NUCLEI ; IODINE 125 ; IODINE ISOTOPES ; Iodine Radioisotopes ; ISOTOPES ; Kidney Diseases - complications ; KIDNEYS ; Longitudinal Studies ; Macromolecular Substances ; MATERIALS ; NUCLEI ; ODD-EVEN NUCLEI ; ORGANIC COMPOUNDS ; ORGANS ; PATIENTS ; PROTEINS ; RADIOASSAY ; RADIOISOTOPES ; RADIOLOGY AND NUCLEAR MEDICINE ; URINE ; UROGENITAL SYSTEM DISEASES ; WASTES</subject><ispartof>Am. J. Med.; (United States), 1983-03, Vol.74 (3), p.375-381</ispartof><rights>1983</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c415t-f8c576a11eae015e315ebde916acb9654941c7403d0d9ca169266cb220d7c72e3</citedby><cites>FETCH-LOGICAL-c415t-f8c576a11eae015e315ebde916acb9654941c7403d0d9ca169266cb220d7c72e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0002-9343(83)90954-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6219575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/5851817$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Valentijn, Robert M.</creatorcontrib><creatorcontrib>Kauffmann, Robert H.</creatorcontrib><creatorcontrib>De La Rivière, Guy Brutel</creatorcontrib><creatorcontrib>Daha, Mohamed R.</creatorcontrib><creatorcontrib>Van Es, Leendert A.</creatorcontrib><creatorcontrib>Department of Nephrology, University Hospital, Leiden, The Netherlands</creatorcontrib><title>Presence of circulating macromolecular IgA in patients with hematuria due to primary IgA nephropathy</title><title>Am. J. Med.; (United States)</title><addtitle>Am J Med</addtitle><description>The relation between renal histologic features and the presence of circulating immune complexes was studied in 50 patients with hematuria. Primary IgA nephropathy was found in 25 patients, and various other forms of glomerulopathy were seen in the remaining 25 patients. Circulating immune complexes were detected with the
125I-C1q-binding assay, the conglutinin-binding assay, and the anti-IgA inhibition binding assay, the latter detecting specifically IgA-containing immune complex-like material. The
125I-C1q-binding assay gave negative findings for all patients except one. With the conglutinin-binding assay, immune complexes were found in a similar frequency for patients with and without IgA nephropathy. However, the anti-IgA inhibition binding assay gave positive results only in patients with primary IgA nephropathy (68 percent) and in none of the other patients. Sucrose density ultracentrifugation, as well as experiments in which the anti-IgA inhibition binding assay was performed with and without pretreatment of serum with polyethylene glycol, showed the presumed IgA immune complexes to have intermediate sedimentation coefficients (11 to 21S). The presence and level of this macromolecular IgA in the circulation correlated significantly (p < 0.001) with the presence of hematuria in patients who had this clinical manifestation intermittently. Furthermore, a significant correlation (r = 0.69, p < 0.0001) was found between the degree of hematuria and the degree of positive findings of the anti-IgA inhibition binding assay. This study shows that in patients presenting with hematuria, a positive finding on the anti-IgA inhibition binding assay is restricted to patients with primary IgA nephropathy and therefore could be of diagnostic value.</description><subject>550601 - Medicine- Unsealed Radionuclides in Diagnostics</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>Binding Sites, Antibody</subject><subject>BIOLOGICAL MATERIALS</subject><subject>BIOLOGICAL WASTES</subject><subject>BLOOD SERUM</subject><subject>BODY</subject><subject>BODY FLUIDS</subject><subject>Centrifugation, Density Gradient</subject><subject>Complement Activating Enzymes - analysis</subject><subject>Complement C1q</subject><subject>Complement Fixation Tests</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DISEASES</subject><subject>ELECTRON CAPTURE RADIOISOTOPES</subject><subject>GLOBULINS</subject><subject>Glomerulonephritis - immunology</subject><subject>Hematuria - etiology</subject><subject>Hematuria - immunology</subject><subject>Humans</subject><subject>Immune Complex Diseases - complications</subject><subject>Immunoglobulin A - analysis</subject><subject>IMMUNOGLOBULINS</subject><subject>INHIBITION</subject><subject>INTERMEDIATE MASS NUCLEI</subject><subject>IODINE 125</subject><subject>IODINE ISOTOPES</subject><subject>Iodine Radioisotopes</subject><subject>ISOTOPES</subject><subject>Kidney Diseases - complications</subject><subject>KIDNEYS</subject><subject>Longitudinal Studies</subject><subject>Macromolecular Substances</subject><subject>MATERIALS</subject><subject>NUCLEI</subject><subject>ODD-EVEN NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PATIENTS</subject><subject>PROTEINS</subject><subject>RADIOASSAY</subject><subject>RADIOISOTOPES</subject><subject>RADIOLOGY AND NUCLEAR MEDICINE</subject><subject>URINE</subject><subject>UROGENITAL SYSTEM DISEASES</subject><subject>WASTES</subject><issn>0002-9343</issn><issn>1555-7162</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvFSEUhYmxqc_qP9CEuDC6mModBhg2TZpGbZMmdaFrwoP7OpiZ4QmMpv9epu-lS10AgfudS-45hLwBdg4M5CfGWNto3vEPPf-omRZdw5-RDQghGgWyfU42T8gL8jLnn_VaMXlKTmULWiixIf5bwoyzQxp31IXkltGWMN_TyboUpzji-pLozf0lDTPd1yLOJdM_oQx0wMmWJQVL_YK0RLpPYbLp4ZGecT-kWAXDwytysrNjxtfH84z8-PL5-9V1c3v39ebq8rZxHYjS7HonlLQAaJGBQF7X1qMGad1WS9HpDpzqGPfMa2dB6lZKt21b5pVTLfIz8u7QN-YSTHahoBtcnGd0xYheQA-qQu8P0D7FXwvmYqaQHY6jnTEu2fSMawV1_x8IXAiuJK9gdwCrYzkn3JmjDwaYWaMyaw5mzcH03DxGZVbZ22P_ZTuhfxIds6n1i0Mdq2W_A6Z1ojUqH9I6kI_h3x_8BYHQoqQ</recordid><startdate>198303</startdate><enddate>198303</enddate><creator>Valentijn, Robert M.</creator><creator>Kauffmann, Robert H.</creator><creator>De La Rivière, Guy Brutel</creator><creator>Daha, Mohamed R.</creator><creator>Van Es, Leendert A.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope><scope>OTOTI</scope></search><sort><creationdate>198303</creationdate><title>Presence of circulating macromolecular IgA in patients with hematuria due to primary IgA nephropathy</title><author>Valentijn, Robert M. ; Kauffmann, Robert H. ; De La Rivière, Guy Brutel ; Daha, Mohamed R. ; Van Es, Leendert A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-f8c576a11eae015e315ebde916acb9654941c7403d0d9ca169266cb220d7c72e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>550601 - Medicine- Unsealed Radionuclides in Diagnostics</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>Binding Sites, Antibody</topic><topic>BIOLOGICAL MATERIALS</topic><topic>BIOLOGICAL WASTES</topic><topic>BLOOD SERUM</topic><topic>BODY</topic><topic>BODY FLUIDS</topic><topic>Centrifugation, Density Gradient</topic><topic>Complement Activating Enzymes - analysis</topic><topic>Complement C1q</topic><topic>Complement Fixation Tests</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DISEASES</topic><topic>ELECTRON CAPTURE RADIOISOTOPES</topic><topic>GLOBULINS</topic><topic>Glomerulonephritis - immunology</topic><topic>Hematuria - etiology</topic><topic>Hematuria - immunology</topic><topic>Humans</topic><topic>Immune Complex Diseases - complications</topic><topic>Immunoglobulin A - analysis</topic><topic>IMMUNOGLOBULINS</topic><topic>INHIBITION</topic><topic>INTERMEDIATE MASS NUCLEI</topic><topic>IODINE 125</topic><topic>IODINE ISOTOPES</topic><topic>Iodine Radioisotopes</topic><topic>ISOTOPES</topic><topic>Kidney Diseases - complications</topic><topic>KIDNEYS</topic><topic>Longitudinal Studies</topic><topic>Macromolecular Substances</topic><topic>MATERIALS</topic><topic>NUCLEI</topic><topic>ODD-EVEN NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PATIENTS</topic><topic>PROTEINS</topic><topic>RADIOASSAY</topic><topic>RADIOISOTOPES</topic><topic>RADIOLOGY AND NUCLEAR MEDICINE</topic><topic>URINE</topic><topic>UROGENITAL SYSTEM DISEASES</topic><topic>WASTES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Valentijn, Robert M.</creatorcontrib><creatorcontrib>Kauffmann, Robert H.</creatorcontrib><creatorcontrib>De La Rivière, Guy Brutel</creatorcontrib><creatorcontrib>Daha, Mohamed R.</creatorcontrib><creatorcontrib>Van Es, Leendert A.</creatorcontrib><creatorcontrib>Department of Nephrology, University Hospital, Leiden, The Netherlands</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><jtitle>Am. J. Med.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Valentijn, Robert M.</au><au>Kauffmann, Robert H.</au><au>De La Rivière, Guy Brutel</au><au>Daha, Mohamed R.</au><au>Van Es, Leendert A.</au><aucorp>Department of Nephrology, University Hospital, Leiden, The Netherlands</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Presence of circulating macromolecular IgA in patients with hematuria due to primary IgA nephropathy</atitle><jtitle>Am. J. Med.; (United States)</jtitle><addtitle>Am J Med</addtitle><date>1983-03</date><risdate>1983</risdate><volume>74</volume><issue>3</issue><spage>375</spage><epage>381</epage><pages>375-381</pages><issn>0002-9343</issn><eissn>1555-7162</eissn><abstract>The relation between renal histologic features and the presence of circulating immune complexes was studied in 50 patients with hematuria. Primary IgA nephropathy was found in 25 patients, and various other forms of glomerulopathy were seen in the remaining 25 patients. Circulating immune complexes were detected with the
125I-C1q-binding assay, the conglutinin-binding assay, and the anti-IgA inhibition binding assay, the latter detecting specifically IgA-containing immune complex-like material. The
125I-C1q-binding assay gave negative findings for all patients except one. With the conglutinin-binding assay, immune complexes were found in a similar frequency for patients with and without IgA nephropathy. However, the anti-IgA inhibition binding assay gave positive results only in patients with primary IgA nephropathy (68 percent) and in none of the other patients. Sucrose density ultracentrifugation, as well as experiments in which the anti-IgA inhibition binding assay was performed with and without pretreatment of serum with polyethylene glycol, showed the presumed IgA immune complexes to have intermediate sedimentation coefficients (11 to 21S). The presence and level of this macromolecular IgA in the circulation correlated significantly (p < 0.001) with the presence of hematuria in patients who had this clinical manifestation intermittently. Furthermore, a significant correlation (r = 0.69, p < 0.0001) was found between the degree of hematuria and the degree of positive findings of the anti-IgA inhibition binding assay. This study shows that in patients presenting with hematuria, a positive finding on the anti-IgA inhibition binding assay is restricted to patients with primary IgA nephropathy and therefore could be of diagnostic value.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6219575</pmid><doi>10.1016/0002-9343(83)90954-3</doi><tpages>7</tpages></addata></record> |
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| ispartof | Am. J. Med.; (United States), 1983-03, Vol.74 (3), p.375-381 |
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| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | 550601 - Medicine- Unsealed Radionuclides in Diagnostics BETA DECAY RADIOISOTOPES Binding Sites, Antibody BIOLOGICAL MATERIALS BIOLOGICAL WASTES BLOOD SERUM BODY BODY FLUIDS Centrifugation, Density Gradient Complement Activating Enzymes - analysis Complement C1q Complement Fixation Tests DAYS LIVING RADIOISOTOPES DISEASES ELECTRON CAPTURE RADIOISOTOPES GLOBULINS Glomerulonephritis - immunology Hematuria - etiology Hematuria - immunology Humans Immune Complex Diseases - complications Immunoglobulin A - analysis IMMUNOGLOBULINS INHIBITION INTERMEDIATE MASS NUCLEI IODINE 125 IODINE ISOTOPES Iodine Radioisotopes ISOTOPES Kidney Diseases - complications KIDNEYS Longitudinal Studies Macromolecular Substances MATERIALS NUCLEI ODD-EVEN NUCLEI ORGANIC COMPOUNDS ORGANS PATIENTS PROTEINS RADIOASSAY RADIOISOTOPES RADIOLOGY AND NUCLEAR MEDICINE URINE UROGENITAL SYSTEM DISEASES WASTES |
| title | Presence of circulating macromolecular IgA in patients with hematuria due to primary IgA nephropathy |
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