Nerve growth factor induces functional nicotinic acetylcholine receptors on rat sensory neurons in culture
Neonatal sensory neurons from rat nodose ganglia express nicotinic acetylcholine receptors when grown in tissue culture without other cell types. The present study investigates the role of nerve growth factor in inducing these receptors. Nerve growth factor has little effect on the growth and surviv...
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| Veröffentlicht in: | Neuroscience 1990, Vol.37 (2), p.523-530 |
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| creator | Mandelzys, A. Cooper, E. Verge, V.M.K. Richardson, P.M. |
| description | Neonatal sensory neurons from rat nodose ganglia express nicotinic acetylcholine receptors when grown in tissue culture without other cell types. The present study investigates the role of nerve growth factor in inducing these receptors. Nerve growth factor has little effect on the growth and survival of nodose neurons in culture, although most neurons were found by quantitative radioautography to have high-affinity nerve growth factor receptors. Nerve growth factor strongly influenced the expression of nicotinic receptors on these neurons: the proportion of acetylcholine-sensitive neurons was approximately 60% in cultures with nerve growth factor compared with 15% in cultures grown without nerve growth factor. The proportion of acetylcholine-sensitive neurons increased over the first week, plateaued by day 12 and remained high for at least three weeks. In contrast, without NGF, the proportion of acetylcholine-sensitive neurons was low throughout the three-week period.
The results indicate that nerve growth factor is an important factor in promoting nicotinic receptors on these neurons in culture. |
| doi_str_mv | 10.1016/0306-4522(90)90420-9 |
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The results indicate that nerve growth factor is an important factor in promoting nicotinic receptors on these neurons in culture.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Autoradiography</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Electrophysiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Iodine Radioisotopes</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Nerve Growth Factors - pharmacology</subject><subject>Neurons, Afferent - drug effects</subject><subject>Neurons, Afferent - metabolism</subject><subject>Nodose Ganglion - cytology</subject><subject>Nodose Ganglion - drug effects</subject><subject>Rats</subject><subject>Receptors, Nicotinic - drug effects</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0306-4522</issn><issn>1873-7544</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGOFCEQhonRrLOrb6AJF40eWqGB7uZiYjaummz0omdCF4XLpgdGoNfM28s4k_WmdaAO9dUf8hUhzzh7wxkf3jLBhk6qvn-l2WvNZM86_YBs-DSKblRSPiSbe-QxOS_llrVSUpyRs54LIdS4IbdfMN8h_ZHTr3pDvYWaMg3RrYCF-jVCDSnahcYAqYb2UgtY9wvcpCVEpBkBd22n0BRptpUWjCXlPY245hRLy6KwLnXN-IQ88nYp-PTUL8j3qw_fLj91118_fr58f92B5FPtwIt5VLO00ssRUDM_y8FL7frZOzZPSvvZcdmj1ygmx7mTFtgMo7AK-16IC_LymLvL6eeKpZptKIDLYiOmtZiJCT2ySf8X5GqcBqbGBsojCDmVktGbXQ5bm_eGM3O4hTmINgfRRjPz5xbmkP_8lL_OW3T3Syf5bf7iNLcF7OKzjRDK32ytxCAG2bh3Rw6btbuA2RQIGAFdaPqrcSn8-yO_AQ73qCM</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Mandelzys, A.</creator><creator>Cooper, E.</creator><creator>Verge, V.M.K.</creator><creator>Richardson, P.M.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Nerve growth factor induces functional nicotinic acetylcholine receptors on rat sensory neurons in culture</title><author>Mandelzys, A. ; Cooper, E. ; Verge, V.M.K. ; Richardson, P.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-cf3b75b4a4f47ce90fb46f49d2bfd0b859fbd142ef9e38d11d4ac0bc73a5e2233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Autoradiography</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Electrophysiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Iodine Radioisotopes</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Nerve Growth Factors - pharmacology</topic><topic>Neurons, Afferent - drug effects</topic><topic>Neurons, Afferent - metabolism</topic><topic>Nodose Ganglion - cytology</topic><topic>Nodose Ganglion - drug effects</topic><topic>Rats</topic><topic>Receptors, Nicotinic - drug effects</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mandelzys, A.</creatorcontrib><creatorcontrib>Cooper, E.</creatorcontrib><creatorcontrib>Verge, V.M.K.</creatorcontrib><creatorcontrib>Richardson, P.M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mandelzys, A.</au><au>Cooper, E.</au><au>Verge, V.M.K.</au><au>Richardson, P.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nerve growth factor induces functional nicotinic acetylcholine receptors on rat sensory neurons in culture</atitle><jtitle>Neuroscience</jtitle><addtitle>Neuroscience</addtitle><date>1990</date><risdate>1990</risdate><volume>37</volume><issue>2</issue><spage>523</spage><epage>530</epage><pages>523-530</pages><issn>0306-4522</issn><eissn>1873-7544</eissn><coden>NRSCDN</coden><abstract>Neonatal sensory neurons from rat nodose ganglia express nicotinic acetylcholine receptors when grown in tissue culture without other cell types. The present study investigates the role of nerve growth factor in inducing these receptors. Nerve growth factor has little effect on the growth and survival of nodose neurons in culture, although most neurons were found by quantitative radioautography to have high-affinity nerve growth factor receptors. Nerve growth factor strongly influenced the expression of nicotinic receptors on these neurons: the proportion of acetylcholine-sensitive neurons was approximately 60% in cultures with nerve growth factor compared with 15% in cultures grown without nerve growth factor. The proportion of acetylcholine-sensitive neurons increased over the first week, plateaued by day 12 and remained high for at least three weeks. In contrast, without NGF, the proportion of acetylcholine-sensitive neurons was low throughout the three-week period.
The results indicate that nerve growth factor is an important factor in promoting nicotinic receptors on these neurons in culture.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>2133357</pmid><doi>10.1016/0306-4522(90)90420-9</doi><tpages>8</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Animals, Newborn Autoradiography Biological and medical sciences Cells, Cultured Electrophysiology Fundamental and applied biological sciences. Psychology Iodine Radioisotopes Isolated neuron and nerve. Neuroglia Nerve Growth Factors - pharmacology Neurons, Afferent - drug effects Neurons, Afferent - metabolism Nodose Ganglion - cytology Nodose Ganglion - drug effects Rats Receptors, Nicotinic - drug effects Vertebrates: nervous system and sense organs |
| title | Nerve growth factor induces functional nicotinic acetylcholine receptors on rat sensory neurons in culture |
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