Linear DNA introduced into carrot protoplasts by electroporation undergoes ligation and recircularization

The integrated DNA in stable transformants formed by direct gene transfer often shows complex restriction patterns. One cause of these complex restriction patterns could be the ligation of plasmid fragments prior to their integration. This paper provides evidence for the ligation of plasmid fragment...

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Veröffentlicht in:Plant molecular biology 1990-06, Vol.14 (6), p.899-908
Hauptverfasser: Bates, G.W. (Florida State Univ., Tallahassee, FL (USA). Dept. of Biological Science), Carle, S.A, Piastuch, W.C
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Sprache:eng
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