chloroplast genome of an exsymbiotic Chlorella-like green alga
Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains...
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| Veröffentlicht in: | Plant molecular biology 1990-05, Vol.14 (5), p.859-862 |
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| container_end_page | 862 |
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| container_title | Plant molecular biology |
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| creator | Schuster, A.M Waddle, J.A Korth, K Meints, R.H |
| description | Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S genes are separated by less than 2000 bp. |
| doi_str_mv | 10.1007/BF00016519 |
| format | Article |
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BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S genes are separated by less than 2000 bp.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/BF00016519</identifier><identifier>PMID: 2102863</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Biological and medical sciences ; Chlorella ; Chlorella - genetics ; chloroplast DNA ; chloroplasts ; Chloroplasts - metabolism ; cistrons ; cloning ; DNA - genetics ; Freshwater ; Fundamental and applied biological sciences. Psychology ; genes ; Genes. 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BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S genes are separated by less than 2000 bp.</description><subject>Biological and medical sciences</subject><subject>Chlorella</subject><subject>Chlorella - genetics</subject><subject>chloroplast DNA</subject><subject>chloroplasts</subject><subject>Chloroplasts - metabolism</subject><subject>cistrons</subject><subject>cloning</subject><subject>DNA - genetics</subject><subject>Freshwater</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>genes</subject><subject>Genes. Genome</subject><subject>genome</subject><subject>genomics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Paramecium</subject><subject>paramecium bursaria</subject><subject>Restriction Mapping</subject><subject>ribosomal RNA</subject><subject>RNA, Ribosomal - genetics</subject><subject>symbionts</subject><subject>Symbiosis</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFLwzAUBvAgypzTi3exFz0I1bykSduLoMOpMPCgO5fXNK3VtJlJB-6_N2NDjx5CQt4vH-Ej5BToNVCa3tzPKKUgBeR7ZAwi5bGgLNsn43CZxkkC7JAcef8REKVcjsiIQQCSj8mtejfW2aVBP0SN7m2nI1tH2Ef626-7srVDq6LpBmljMDbtp44ap3UfoWnwmBzUaLw-2e0Tspg9vE2f4vnL4_P0bh4rLtMhLjlQgZpJTJhIlcxYWDVTSCshsSp5CZBBUud5BZIKnlOeKcZ4ySpRImR8Qi63uUtnv1baD0XXerX5Ua_tyhdZeMAFy_-FICRnGYcAr7ZQOeu903WxdG2Hbl0ALTatFn-tBny2S12Vna5-6a7GML_YzdErNLXDXrX-LzFPEsESGdz51tVoC2xcMItXRoFTSHkO4fADY4mFaQ</recordid><startdate>19900501</startdate><enddate>19900501</enddate><creator>Schuster, A.M</creator><creator>Waddle, J.A</creator><creator>Korth, K</creator><creator>Meints, R.H</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19900501</creationdate><title>chloroplast genome of an exsymbiotic Chlorella-like green alga</title><author>Schuster, A.M ; Waddle, J.A ; Korth, K ; Meints, R.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c367t-b3105ae26a4257c682c68f2ca0d56adb3b11814f99d160539038c223b2d5ba183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Chlorella</topic><topic>Chlorella - genetics</topic><topic>chloroplast DNA</topic><topic>chloroplasts</topic><topic>Chloroplasts - metabolism</topic><topic>cistrons</topic><topic>cloning</topic><topic>DNA - genetics</topic><topic>Freshwater</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>genes</topic><topic>Genes. Genome</topic><topic>genome</topic><topic>genomics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Paramecium</topic><topic>paramecium bursaria</topic><topic>Restriction Mapping</topic><topic>ribosomal RNA</topic><topic>RNA, Ribosomal - genetics</topic><topic>symbionts</topic><topic>Symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schuster, A.M</creatorcontrib><creatorcontrib>Waddle, J.A</creatorcontrib><creatorcontrib>Korth, K</creatorcontrib><creatorcontrib>Meints, R.H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schuster, A.M</au><au>Waddle, J.A</au><au>Korth, K</au><au>Meints, R.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>chloroplast genome of an exsymbiotic Chlorella-like green alga</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1990-05-01</date><risdate>1990</risdate><volume>14</volume><issue>5</issue><spage>859</spage><epage>862</epage><pages>859-862</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S genes are separated by less than 2000 bp.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>2102863</pmid><doi>10.1007/BF00016519</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0167-4412 |
| ispartof | Plant molecular biology, 1990-05, Vol.14 (5), p.859-862 |
| issn | 0167-4412 1573-5028 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Biological and medical sciences Chlorella Chlorella - genetics chloroplast DNA chloroplasts Chloroplasts - metabolism cistrons cloning DNA - genetics Freshwater Fundamental and applied biological sciences. Psychology genes Genes. Genome genome genomics Molecular and cellular biology Molecular genetics Paramecium paramecium bursaria Restriction Mapping ribosomal RNA RNA, Ribosomal - genetics symbionts Symbiosis |
| title | chloroplast genome of an exsymbiotic Chlorella-like green alga |
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