chloroplast genome of an exsymbiotic Chlorella-like green alga

Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains...

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Veröffentlicht in:	Plant molecular biology 1990-05, Vol.14 (5), p.859-862
Hauptverfasser:	Schuster, A.M, Waddle, J.A, Korth, K, Meints, R.H
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Chlorella Chlorella - genetics chloroplast DNA chloroplasts Chloroplasts - metabolism cistrons cloning DNA - genetics Freshwater Fundamental and applied biological sciences. Psychology genes Genes. Genome genome genomics Molecular and cellular biology Molecular genetics Paramecium paramecium bursaria Restriction Mapping ribosomal RNA RNA, Ribosomal - genetics symbionts Symbiosis
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Beschreibung
Zusammenfassung:	Chloroplast DNA was isolated and cloned from Chlorella, strain N1a, exsymbiotic with Paramecium bursaria. BamHI, SalI, Sst1, KpnI and XhoI restriction fragments of the DNA were assembled into a circular map. The genome consists of approximately 120 kbp of DNA, has a G/C content of 38%, and contains only a single copy of the rRNA cistron. The rRNA cistron is small, 5000-8000 bp, and the 16S and 23S genes are separated by less than 2000 bp.
ISSN:	0167-4412 1573-5028
DOI:	10.1007/BF00016519