Identification of two forms of myosin light chain kinase in turkey gizzard

Two forms of myosin light chain kinase from turkey gizzard are separable by ion-exchange chromatography. One is the well-characterized 13 000 M r enzyme. Purification of the second form by affinity chromatography on calmodulin-Sepharose showed it to consist of two polypeptide chains of M r 136 000 a...

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Veröffentlicht in:	FEBS letters 1983-03, Vol.153 (1), p.156-160
Hauptverfasser:	Walsh, Michael P., Hinkins, Susan, Muguruma, Michio, Hartshorne, David J.
Format:	Artikel
Sprache:	eng
Schlagworte:	adenosine 5′-O-(3-thiotriphosphate) Animals ATPγS Calcium Calcium - pharmacology Calmodulin Calmodulin - pharmacology chickens Chromatography, Affinity Chromatography, Ion Exchange CTP cytidine 5′-triphosphate dithiothreitol DTT EGTA Electrophoresis, Polyacrylamide Gel ethylene glycol bis (β-aminoethyl ether)N,N,N′,N′-tetraacetic acid gizzard Gizzard, Avian - enzymology inosine 5′-triphosphate ITP MLCK Myosin kinase Myosin-Light-Chain Kinase Myosins - metabolism PAGE Phosphorylation polyacrylamide gel electrophoresis Protein Kinases - isolation & purification Protein Kinases - metabolism Regulation SDS Smooth muscle sodium dodecyl sulfate Substrate Specificity Turkeys - metabolism
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