Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography

Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography

A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtrati...

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Veröffentlicht in:Journal of immunological methods 1983-02, Vol.57 (1), p.197-204
Hauptverfasser: Cripps, A.W., Neoh, S.H., Smart, I.J.
Format: Artikel
Sprache:eng
Schlagworte:
affinity chromatography
Chemical Precipitation
Chromatography, Affinity
Humans
IgA
IgM
Immunoglobulin A - isolation & purification
Immunoglobulin M - isolation & purification
PEG-6000
Polyethylene Glycols
purification
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container_title Journal of immunological methods
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creator Cripps, A.W.
Neoh, S.H.
Smart, I.J.
description A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtration. The final recovery of both IgA and IgM was approximately 30%. The purified preparations obtained were characterized by immunoelectrophoresis, double immunodiffusion, radial immunodiffusion, radioimmunoassay and gel filtration.
doi_str_mv 10.1016/0022-1759(83)90078-9
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subjects affinity chromatography
Chemical Precipitation
Chromatography, Affinity
Humans
IgA
IgM
Immunoglobulin A - isolation & purification
Immunoglobulin M - isolation & purification
PEG-6000
Polyethylene Glycols
purification
title Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography
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