The need for a sufficient number of low level sera in comparisons of different serum vitamin B12 assays
Eight radiochemical methods for the assay of vitamin B12 in serum were compared with the microbiological assay with Lactobacillus leichmannii ATCC 7830 using 198 individual sera of patients. There was a good agreement between the results of most samples with some kits and the microbiological assay....
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| Veröffentlicht in: | Clinica chimica acta 1983-01, Vol.127 (2), p.185-195 |
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| container_issue | 2 |
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| container_title | Clinica chimica acta |
| container_volume | 127 |
| creator | Gijzen, A.H.J. de Kock, H.W. Meulendijk, P.N. Schmidt, N.A. Schopman, W. Tertoolen, J.F.W. Voogd, C.E. |
| description | Eight radiochemical methods for the assay of vitamin B12 in serum were compared with the microbiological assay with Lactobacillus leichmannii ATCC 7830 using 198 individual sera of patients.
There was a good agreement between the results of most samples with some kits and the microbiological assay. However, especially in the sera of vitamin B12-deficient patients large discrepancies between the results could occur. These variations were due to both the kits used and the performance of the assays in different laboratories.
A sufficient number of non-pooled sera of vitamin B12-deficient patients should be included in investigations to validate radiochemical methods. |
| doi_str_mv | 10.1016/S0009-8981(83)80003-5 |
| format | Article |
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There was a good agreement between the results of most samples with some kits and the microbiological assay. However, especially in the sera of vitamin B12-deficient patients large discrepancies between the results could occur. These variations were due to both the kits used and the performance of the assays in different laboratories.
A sufficient number of non-pooled sera of vitamin B12-deficient patients should be included in investigations to validate radiochemical methods.</description><identifier>ISSN: 0009-8981</identifier><identifier>EISSN: 1873-3492</identifier><identifier>DOI: 10.1016/S0009-8981(83)80003-5</identifier><identifier>PMID: 6825315</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Biological Assay ; Humans ; Lactobacillus ; Methods ; Microchemistry ; Radioisotopes ; Reagent Kits, Diagnostic - standards ; Vitamin B 12 - blood ; Vitamin B 12 Deficiency - blood</subject><ispartof>Clinica chimica acta, 1983-01, Vol.127 (2), p.185-195</ispartof><rights>1983 Elsevier Biomedical Press</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c360t-282940ddbc0481e88a6a35a0ddeb699f60340f21f8ff587411c960f66407a1e23</citedby><cites>FETCH-LOGICAL-c360t-282940ddbc0481e88a6a35a0ddeb699f60340f21f8ff587411c960f66407a1e23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0009-8981(83)80003-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6825315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>de Kock, H.W.</creatorcontrib><creatorcontrib>Meulendijk, P.N.</creatorcontrib><creatorcontrib>Schmidt, N.A.</creatorcontrib><creatorcontrib>Schopman, W.</creatorcontrib><creatorcontrib>Tertoolen, J.F.W.</creatorcontrib><creatorcontrib>Voogd, C.E.</creatorcontrib><title>The need for a sufficient number of low level sera in comparisons of different serum vitamin B12 assays</title><title>Clinica chimica acta</title><addtitle>Clin Chim Acta</addtitle><description>Eight radiochemical methods for the assay of vitamin B12 in serum were compared with the microbiological assay with Lactobacillus leichmannii ATCC 7830 using 198 individual sera of patients.
There was a good agreement between the results of most samples with some kits and the microbiological assay. However, especially in the sera of vitamin B12-deficient patients large discrepancies between the results could occur. These variations were due to both the kits used and the performance of the assays in different laboratories.
A sufficient number of non-pooled sera of vitamin B12-deficient patients should be included in investigations to validate radiochemical methods.</description><subject>Biological Assay</subject><subject>Humans</subject><subject>Lactobacillus</subject><subject>Methods</subject><subject>Microchemistry</subject><subject>Radioisotopes</subject><subject>Reagent Kits, Diagnostic - standards</subject><subject>Vitamin B 12 - blood</subject><subject>Vitamin B 12 Deficiency - blood</subject><issn>0009-8981</issn><issn>1873-3492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1PwzAQhi0EKqXwEyp5QjAE7DhxnQlBxZdUiYEyW45zBqMkLnZS1H-P01ZdmazzPe-d7kFoSskNJZTfvhNCikQUgl4Jdi1ixZL8CI2pmLGEZUV6jMYH5BSdhfAdy4xwOkIjLtKc0XyMPpdfgFuAChvnscKhN8ZqC22H274pwWNncO1-cQ1rqHEAr7BtsXbNSnkbXBsGoLLGgB9CEegbvLadaiL2QFOsQlCbcI5OjKoDXOzfCfp4elzOX5LF2_Pr_H6RaMZJl6QiLTJSVaUmmaAghOKK5Sr-QMmLwnDCMmJSaoQxuZhllOqCE8N5RmaKQsom6HI3d-XdTw-hk40NGupateD6IAVhM8aFiGC-A7V3IXgwcuVto_xGUiIHwXIrWA72pGByK1jmMTfdL-jLBqpDam809u92fYhXri14GQadGirrQXeycvafDX95jIov</recordid><startdate>19830124</startdate><enddate>19830124</enddate><creator>Gijzen, A.H.J.</creator><creator>de Kock, H.W.</creator><creator>Meulendijk, P.N.</creator><creator>Schmidt, N.A.</creator><creator>Schopman, W.</creator><creator>Tertoolen, J.F.W.</creator><creator>Voogd, C.E.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19830124</creationdate><title>The need for a sufficient number of low level sera in comparisons of different serum vitamin B12 assays</title><author>Gijzen, A.H.J. ; de Kock, H.W. ; Meulendijk, P.N. ; Schmidt, N.A. ; Schopman, W. ; Tertoolen, J.F.W. ; Voogd, C.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c360t-282940ddbc0481e88a6a35a0ddeb699f60340f21f8ff587411c960f66407a1e23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Biological Assay</topic><topic>Humans</topic><topic>Lactobacillus</topic><topic>Methods</topic><topic>Microchemistry</topic><topic>Radioisotopes</topic><topic>Reagent Kits, Diagnostic - standards</topic><topic>Vitamin B 12 - blood</topic><topic>Vitamin B 12 Deficiency - blood</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gijzen, A.H.J.</creatorcontrib><creatorcontrib>de Kock, H.W.</creatorcontrib><creatorcontrib>Meulendijk, P.N.</creatorcontrib><creatorcontrib>Schmidt, N.A.</creatorcontrib><creatorcontrib>Schopman, W.</creatorcontrib><creatorcontrib>Tertoolen, J.F.W.</creatorcontrib><creatorcontrib>Voogd, C.E.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gijzen, A.H.J.</au><au>de Kock, H.W.</au><au>Meulendijk, P.N.</au><au>Schmidt, N.A.</au><au>Schopman, W.</au><au>Tertoolen, J.F.W.</au><au>Voogd, C.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The need for a sufficient number of low level sera in comparisons of different serum vitamin B12 assays</atitle><jtitle>Clinica chimica acta</jtitle><addtitle>Clin Chim Acta</addtitle><date>1983-01-24</date><risdate>1983</risdate><volume>127</volume><issue>2</issue><spage>185</spage><epage>195</epage><pages>185-195</pages><issn>0009-8981</issn><eissn>1873-3492</eissn><abstract>Eight radiochemical methods for the assay of vitamin B12 in serum were compared with the microbiological assay with Lactobacillus leichmannii ATCC 7830 using 198 individual sera of patients.
There was a good agreement between the results of most samples with some kits and the microbiological assay. However, especially in the sera of vitamin B12-deficient patients large discrepancies between the results could occur. These variations were due to both the kits used and the performance of the assays in different laboratories.
A sufficient number of non-pooled sera of vitamin B12-deficient patients should be included in investigations to validate radiochemical methods.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6825315</pmid><doi>10.1016/S0009-8981(83)80003-5</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0009-8981 |
| ispartof | Clinica chimica acta, 1983-01, Vol.127 (2), p.185-195 |
| issn | 0009-8981 1873-3492 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Biological Assay Humans Lactobacillus Methods Microchemistry Radioisotopes Reagent Kits, Diagnostic - standards Vitamin B 12 - blood Vitamin B 12 Deficiency - blood |
| title | The need for a sufficient number of low level sera in comparisons of different serum vitamin B12 assays |
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