Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin
In 1983, a gonococcal pilus vaccine failed to show protection in a large, placebo-controlled, double-blind field trial. The epitopic response to this vaccine was investigated in a random subgroup of 20 vaccine recipients. Using Western blot analysis of the immunizing pilus and its cyanogen bromide (...
Gespeichert in:
| Veröffentlicht in: | The Journal of infectious diseases 1991-01, Vol.163 (1), p.128-134 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 134 |
|---|---|
| container_issue | 1 |
| container_start_page | 128 |
| container_title | The Journal of infectious diseases |
| container_volume | 163 |
| creator | Johnson, Steven C. Chung, Raymond C. Y. Deal, Carolyn D. Boslego, John W. Sadoff, Jerald C. Wood, Sarah W. Brinton, Charles C. Tramont, Edmund C. |
| description | In 1983, a gonococcal pilus vaccine failed to show protection in a large, placebo-controlled, double-blind field trial. The epitopic response to this vaccine was investigated in a random subgroup of 20 vaccine recipients. Using Western blot analysis of the immunizing pilus and its cyanogen bromide (CNBr) fragments, IgG antibody to pilin was detected before immunization in all individuals. Preexistent antibody to the CNBr-2 and CNBr-3 fragments ofpilin wasdetected in 65% and 5% of individuals, respectively. Pilus immunization resulted in a vigorous response to the CNBr-2 fragment in 100% of the individuals tested; only 33% developedantibody to the CNBr-3 fragment. Absorptions ofpostimmunization sera with different gonococcal strains resulted in either complete or partial removal of antibody to the CNBr-2 fragment. In the context of an unsuccessful vaccine trial, these results suggest that antibody to the CNBr-2 fragment of pilin may not be protective. |
| doi_str_mv | 10.1093/infdis/163.1.128 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_80368272</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30119521</jstor_id><sourcerecordid>30119521</sourcerecordid><originalsourceid>FETCH-LOGICAL-c414t-3ad549cb60d75f09945be6c11d9efef0e6af498eda8d2de3f0c7373673afcd13</originalsourceid><addsrcrecordid>eNqFkb9v1DAcxS0EKtfCzoLkAbHlaseJnYwl4nqVWqhKB8Ri-fzjziWxi-1QjoV_HUc52pHpO7z3PtL3PQDeYLTEqCWn1hll4ymmZImXuGyegQWuCSsoxeQ5WCBUlgVu2vYlOI7xDiFUEcqOwBFmCDeYLcCf9TgIBy-GYXT2t0jWO_hg0w5eb3eQFCU8985LL6Xo4bXtxwhvdBz7FKF1sAs-xuJGC5nsTw3PXLIbr_YweZh2GnZ74fxWO_gh-MEqDVdBbAftUsZ6M-GsewVeGNFH_fpwT8Dt6uNtty4uP59fdGeXhaxwlQoiVF21ckORYrVBbVvVG00lxqrVRhukqTBV22glGlUqTQySjLD8KxFGKkxOwPsZex_8j1HHxAcbpe574bQfI28QoU3Jyv8acd3kPsvJiGajnEoI2vD7YAcR9hwjPm3D52143oZjnrfJkbcH9rgZtHoKzGNk_d1BFzH3bYJwMgP-2aqWNqymT5i7mHx4lAnCuK3L6ddi1m1M-tejLsJ3nvtgNV9__cZRt0KfyJcrfkX-AhmAse0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>15818922</pqid></control><display><type>article</type><title>Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><creator>Johnson, Steven C. ; Chung, Raymond C. Y. ; Deal, Carolyn D. ; Boslego, John W. ; Sadoff, Jerald C. ; Wood, Sarah W. ; Brinton, Charles C. ; Tramont, Edmund C.</creator><creatorcontrib>Johnson, Steven C. ; Chung, Raymond C. Y. ; Deal, Carolyn D. ; Boslego, John W. ; Sadoff, Jerald C. ; Wood, Sarah W. ; Brinton, Charles C. ; Tramont, Edmund C.</creatorcontrib><description>In 1983, a gonococcal pilus vaccine failed to show protection in a large, placebo-controlled, double-blind field trial. The epitopic response to this vaccine was investigated in a random subgroup of 20 vaccine recipients. Using Western blot analysis of the immunizing pilus and its cyanogen bromide (CNBr) fragments, IgG antibody to pilin was detected before immunization in all individuals. Preexistent antibody to the CNBr-2 and CNBr-3 fragments ofpilin wasdetected in 65% and 5% of individuals, respectively. Pilus immunization resulted in a vigorous response to the CNBr-2 fragment in 100% of the individuals tested; only 33% developedantibody to the CNBr-3 fragment. Absorptions ofpostimmunization sera with different gonococcal strains resulted in either complete or partial removal of antibody to the CNBr-2 fragment. In the context of an unsuccessful vaccine trial, these results suggest that antibody to the CNBr-2 fragment of pilin may not be protective.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/163.1.128</identifier><identifier>PMID: 1701817</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Adult ; Amino Acid Sequence ; Amino acids ; Antibodies ; Antibodies, Bacterial - biosynthesis ; Bacterial Outer Membrane Proteins - immunology ; Bacterial Vaccines - immunology ; Bacteriology ; Biological and medical sciences ; Blotting, Western ; Bromides ; Cross Reactions ; Cyanogen Bromide ; Double-Blind Method ; Enzyme-Linked Immunosorbent Assay ; Epitopes - immunology ; Experimentation ; Female ; Fimbriae Proteins ; Fimbriae, Bacterial - immunology ; Fundamental and applied biological sciences. Psychology ; Gonorrhea ; Gonorrhea - prevention & control ; Humans ; Immunization ; Major Articles ; Male ; Microbiology ; Molecular Sequence Data ; Molecules ; Neisseria gonorrhoeae - immunology ; Neisseria gonorrhoeae - ultrastructure ; Peptide Fragments - immunology ; Vaccination ; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies ; Volunteerism</subject><ispartof>The Journal of infectious diseases, 1991-01, Vol.163 (1), p.128-134</ispartof><rights>Copyright 1991 The University of Chicago</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c414t-3ad549cb60d75f09945be6c11d9efef0e6af498eda8d2de3f0c7373673afcd13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30119521$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30119521$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,4010,27900,27901,27902,57992,58225</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4968756$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1701817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnson, Steven C.</creatorcontrib><creatorcontrib>Chung, Raymond C. Y.</creatorcontrib><creatorcontrib>Deal, Carolyn D.</creatorcontrib><creatorcontrib>Boslego, John W.</creatorcontrib><creatorcontrib>Sadoff, Jerald C.</creatorcontrib><creatorcontrib>Wood, Sarah W.</creatorcontrib><creatorcontrib>Brinton, Charles C.</creatorcontrib><creatorcontrib>Tramont, Edmund C.</creatorcontrib><title>Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>In 1983, a gonococcal pilus vaccine failed to show protection in a large, placebo-controlled, double-blind field trial. The epitopic response to this vaccine was investigated in a random subgroup of 20 vaccine recipients. Using Western blot analysis of the immunizing pilus and its cyanogen bromide (CNBr) fragments, IgG antibody to pilin was detected before immunization in all individuals. Preexistent antibody to the CNBr-2 and CNBr-3 fragments ofpilin wasdetected in 65% and 5% of individuals, respectively. Pilus immunization resulted in a vigorous response to the CNBr-2 fragment in 100% of the individuals tested; only 33% developedantibody to the CNBr-3 fragment. Absorptions ofpostimmunization sera with different gonococcal strains resulted in either complete or partial removal of antibody to the CNBr-2 fragment. In the context of an unsuccessful vaccine trial, these results suggest that antibody to the CNBr-2 fragment of pilin may not be protective.</description><subject>Adult</subject><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Antibodies</subject><subject>Antibodies, Bacterial - biosynthesis</subject><subject>Bacterial Outer Membrane Proteins - immunology</subject><subject>Bacterial Vaccines - immunology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Bromides</subject><subject>Cross Reactions</subject><subject>Cyanogen Bromide</subject><subject>Double-Blind Method</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Epitopes - immunology</subject><subject>Experimentation</subject><subject>Female</subject><subject>Fimbriae Proteins</subject><subject>Fimbriae, Bacterial - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gonorrhea</subject><subject>Gonorrhea - prevention & control</subject><subject>Humans</subject><subject>Immunization</subject><subject>Major Articles</subject><subject>Male</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Neisseria gonorrhoeae - immunology</subject><subject>Neisseria gonorrhoeae - ultrastructure</subject><subject>Peptide Fragments - immunology</subject><subject>Vaccination</subject><subject>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</subject><subject>Volunteerism</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb9v1DAcxS0EKtfCzoLkAbHlaseJnYwl4nqVWqhKB8Ri-fzjziWxi-1QjoV_HUc52pHpO7z3PtL3PQDeYLTEqCWn1hll4ymmZImXuGyegQWuCSsoxeQ5WCBUlgVu2vYlOI7xDiFUEcqOwBFmCDeYLcCf9TgIBy-GYXT2t0jWO_hg0w5eb3eQFCU8985LL6Xo4bXtxwhvdBz7FKF1sAs-xuJGC5nsTw3PXLIbr_YweZh2GnZ74fxWO_gh-MEqDVdBbAftUsZ6M-GsewVeGNFH_fpwT8Dt6uNtty4uP59fdGeXhaxwlQoiVF21ckORYrVBbVvVG00lxqrVRhukqTBV22glGlUqTQySjLD8KxFGKkxOwPsZex_8j1HHxAcbpe574bQfI28QoU3Jyv8acd3kPsvJiGajnEoI2vD7YAcR9hwjPm3D52143oZjnrfJkbcH9rgZtHoKzGNk_d1BFzH3bYJwMgP-2aqWNqymT5i7mHx4lAnCuK3L6ddi1m1M-tejLsJ3nvtgNV9__cZRt0KfyJcrfkX-AhmAse0</recordid><startdate>199101</startdate><enddate>199101</enddate><creator>Johnson, Steven C.</creator><creator>Chung, Raymond C. Y.</creator><creator>Deal, Carolyn D.</creator><creator>Boslego, John W.</creator><creator>Sadoff, Jerald C.</creator><creator>Wood, Sarah W.</creator><creator>Brinton, Charles C.</creator><creator>Tramont, Edmund C.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199101</creationdate><title>Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin</title><author>Johnson, Steven C. ; Chung, Raymond C. Y. ; Deal, Carolyn D. ; Boslego, John W. ; Sadoff, Jerald C. ; Wood, Sarah W. ; Brinton, Charles C. ; Tramont, Edmund C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c414t-3ad549cb60d75f09945be6c11d9efef0e6af498eda8d2de3f0c7373673afcd13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Adult</topic><topic>Amino Acid Sequence</topic><topic>Amino acids</topic><topic>Antibodies</topic><topic>Antibodies, Bacterial - biosynthesis</topic><topic>Bacterial Outer Membrane Proteins - immunology</topic><topic>Bacterial Vaccines - immunology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Bromides</topic><topic>Cross Reactions</topic><topic>Cyanogen Bromide</topic><topic>Double-Blind Method</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Epitopes - immunology</topic><topic>Experimentation</topic><topic>Female</topic><topic>Fimbriae Proteins</topic><topic>Fimbriae, Bacterial - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gonorrhea</topic><topic>Gonorrhea - prevention & control</topic><topic>Humans</topic><topic>Immunization</topic><topic>Major Articles</topic><topic>Male</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Neisseria gonorrhoeae - immunology</topic><topic>Neisseria gonorrhoeae - ultrastructure</topic><topic>Peptide Fragments - immunology</topic><topic>Vaccination</topic><topic>Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies</topic><topic>Volunteerism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnson, Steven C.</creatorcontrib><creatorcontrib>Chung, Raymond C. Y.</creatorcontrib><creatorcontrib>Deal, Carolyn D.</creatorcontrib><creatorcontrib>Boslego, John W.</creatorcontrib><creatorcontrib>Sadoff, Jerald C.</creatorcontrib><creatorcontrib>Wood, Sarah W.</creatorcontrib><creatorcontrib>Brinton, Charles C.</creatorcontrib><creatorcontrib>Tramont, Edmund C.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnson, Steven C.</au><au>Chung, Raymond C. Y.</au><au>Deal, Carolyn D.</au><au>Boslego, John W.</au><au>Sadoff, Jerald C.</au><au>Wood, Sarah W.</au><au>Brinton, Charles C.</au><au>Tramont, Edmund C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1991-01</date><risdate>1991</risdate><volume>163</volume><issue>1</issue><spage>128</spage><epage>134</epage><pages>128-134</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>In 1983, a gonococcal pilus vaccine failed to show protection in a large, placebo-controlled, double-blind field trial. The epitopic response to this vaccine was investigated in a random subgroup of 20 vaccine recipients. Using Western blot analysis of the immunizing pilus and its cyanogen bromide (CNBr) fragments, IgG antibody to pilin was detected before immunization in all individuals. Preexistent antibody to the CNBr-2 and CNBr-3 fragments ofpilin wasdetected in 65% and 5% of individuals, respectively. Pilus immunization resulted in a vigorous response to the CNBr-2 fragment in 100% of the individuals tested; only 33% developedantibody to the CNBr-3 fragment. Absorptions ofpostimmunization sera with different gonococcal strains resulted in either complete or partial removal of antibody to the CNBr-2 fragment. In the context of an unsuccessful vaccine trial, these results suggest that antibody to the CNBr-2 fragment of pilin may not be protective.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>1701817</pmid><doi>10.1093/infdis/163.1.128</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1991-01, Vol.163 (1), p.128-134 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80368272 |
| source | Jstor Complete Legacy; MEDLINE |
| subjects | Adult Amino Acid Sequence Amino acids Antibodies Antibodies, Bacterial - biosynthesis Bacterial Outer Membrane Proteins - immunology Bacterial Vaccines - immunology Bacteriology Biological and medical sciences Blotting, Western Bromides Cross Reactions Cyanogen Bromide Double-Blind Method Enzyme-Linked Immunosorbent Assay Epitopes - immunology Experimentation Female Fimbriae Proteins Fimbriae, Bacterial - immunology Fundamental and applied biological sciences. Psychology Gonorrhea Gonorrhea - prevention & control Humans Immunization Major Articles Male Microbiology Molecular Sequence Data Molecules Neisseria gonorrhoeae - immunology Neisseria gonorrhoeae - ultrastructure Peptide Fragments - immunology Vaccination Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies Volunteerism |
| title | Human Immunization with Pgh 3-2 Gonococcal Pilus Results in Cross-Reactive Antibody to the Cyanogen Bromide Fragment-2 of Pilin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-12T22%3A06%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Human%20Immunization%20with%20Pgh%203-2%20Gonococcal%20Pilus%20Results%20in%20Cross-Reactive%20Antibody%20to%20the%20Cyanogen%20Bromide%20Fragment-2%20of%20Pilin&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Johnson,%20Steven%20C.&rft.date=1991-01&rft.volume=163&rft.issue=1&rft.spage=128&rft.epage=134&rft.pages=128-134&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/10.1093/infdis/163.1.128&rft_dat=%3Cjstor_proqu%3E30119521%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=15818922&rft_id=info:pmid/1701817&rft_jstor_id=30119521&rfr_iscdi=true |