Chromatographic Resolution and Kinetic Characterization of Glucokinase from Islets of Langerhans

Glucokinase (ATP:D-glucose 6-phosphotransferase, EC 2.7.1.2) from rat islets of Langerhans was partially purified by chromatography on DEAE-Cibacron blue F3GA agarose. The enzyme eluted in two separate peaks. Sigmoidal rate dependence was found with respect to glucose (Hill coefficient = 1.5) for bo...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1983-01, Vol.80 (1), p.85-89
Hauptverfasser:	Meglasson, Martin D., Burch, Pamela Trueheart, Berner, Donna K., Najafi, Habiba, Vogin, Alan P., Matschinsky, Franz M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry Brain - enzymology Chromatography DNA Elution Enzymes glucokinase Glucokinase - isolation & purification Glucokinase - metabolism Hexokinase - metabolism Homogenization Islets of Langerhans Islets of Langerhans - enzymology Kinetics Liver Liver - enzymology Male Phosphorylation Rats
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!