Increased Procollagen mRNA Levels in Scleroderma Skin Fibroblasts
Procollagen messenger RNA activity in scleroderma and normal skin fibroblasts was measured using a cell-free translation assay. Radioactive translation products were fractionated by electrophoresis and the ratio of procollagen to total incorporation was determined from densitometric scans of gel flu...
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| Veröffentlicht in: | Journal of investigative dermatology 1983-02, Vol.80 (2), p.130-132 |
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| container_title | Journal of investigative dermatology |
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| creator | Graves, Peter N. Weiss, Irwin K. Perlish, Jerome S. Fleischmajer, Raul |
| description | Procollagen messenger RNA activity in scleroderma and normal skin fibroblasts was measured using a cell-free translation assay. Radioactive translation products were fractionated by electrophoresis and the ratio of procollagen to total incorporation was determined from densitometric scans of gel fluorograms. In 4 scleroderma cell lines 1.78% (± 0.10) of incorporated [35S]-methionine was in procollagen, compared to 1.00% (± 0.20) in 5 normal controls, These values are consistent with previously reported increases in the rates of collagen synthesis obtained with intact cells and show that most if not all of the increase can be explained on the basis of elevated translatable procollagen messenger RNA in scleroderma fibroblasts. |
| doi_str_mv | 10.1111/1523-1747.ep12532908 |
| format | Article |
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Radioactive translation products were fractionated by electrophoresis and the ratio of procollagen to total incorporation was determined from densitometric scans of gel fluorograms. In 4 scleroderma cell lines 1.78% (± 0.10) of incorporated [35S]-methionine was in procollagen, compared to 1.00% (± 0.20) in 5 normal controls, These values are consistent with previously reported increases in the rates of collagen synthesis obtained with intact cells and show that most if not all of the increase can be explained on the basis of elevated translatable procollagen messenger RNA in scleroderma fibroblasts.</description><identifier>ISSN: 0022-202X</identifier><identifier>EISSN: 1523-1747</identifier><identifier>DOI: 10.1111/1523-1747.ep12532908</identifier><identifier>PMID: 6822742</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adult ; Animals ; Cells, Cultured ; Female ; Fibroblasts - metabolism ; Humans ; Male ; Middle Aged ; Procollagen - genetics ; Protein Biosynthesis ; Rabbits ; Reticulocytes - metabolism ; RNA, Messenger - genetics ; Scleroderma, Systemic - metabolism ; Skin - metabolism</subject><ispartof>Journal of investigative dermatology, 1983-02, Vol.80 (2), p.130-132</ispartof><rights>1983 The Society for Investigative Dermatology, Inc</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3408-a60624e5b4c13cd158bf81a4b410ab0f54d4cae7e5e1b056dd5e201563b8ebcc3</citedby><cites>FETCH-LOGICAL-c3408-a60624e5b4c13cd158bf81a4b410ab0f54d4cae7e5e1b056dd5e201563b8ebcc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6822742$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Graves, Peter N.</creatorcontrib><creatorcontrib>Weiss, Irwin K.</creatorcontrib><creatorcontrib>Perlish, Jerome S.</creatorcontrib><creatorcontrib>Fleischmajer, Raul</creatorcontrib><title>Increased Procollagen mRNA Levels in Scleroderma Skin Fibroblasts</title><title>Journal of investigative dermatology</title><addtitle>J Invest Dermatol</addtitle><description>Procollagen messenger RNA activity in scleroderma and normal skin fibroblasts was measured using a cell-free translation assay. Radioactive translation products were fractionated by electrophoresis and the ratio of procollagen to total incorporation was determined from densitometric scans of gel fluorograms. In 4 scleroderma cell lines 1.78% (± 0.10) of incorporated [35S]-methionine was in procollagen, compared to 1.00% (± 0.20) in 5 normal controls, These values are consistent with previously reported increases in the rates of collagen synthesis obtained with intact cells and show that most if not all of the increase can be explained on the basis of elevated translatable procollagen messenger RNA in scleroderma fibroblasts.</description><subject>Adult</subject><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Female</subject><subject>Fibroblasts - metabolism</subject><subject>Humans</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Procollagen - genetics</subject><subject>Protein Biosynthesis</subject><subject>Rabbits</subject><subject>Reticulocytes - metabolism</subject><subject>RNA, Messenger - genetics</subject><subject>Scleroderma, Systemic - metabolism</subject><subject>Skin - metabolism</subject><issn>0022-202X</issn><issn>1523-1747</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtLA0EQhAdRYoz-A4U9eds4z93JRQhiNBBUjIK3YR69MrqPOLMb8N-7ISG52ZeGqq6i-RC6JHhM-rkhgrKU5Dwfw4pQwegEyyM03MvHaIgxpSnF9OMUncX4hTHJuJADNMgkpTmnQzSd1zaAjuCSl9DYpiz1J9RJ9fo0TRawhjImvk6WtoTQOAiVTpbfvTDzJjSm1LGN5-ik0GWEi90eoffZ_dvdY7p4fpjfTRepZRzLVGc4oxyE4ZYw64iQppBEc8MJ1gYXgjtuNeQggBgsMucEUExExowEYy0boett7yo0Px3EVlU-WugfrqHpopKYiYlgpD_k20MbmhgDFGoVfKXDryJYbcipDSK1QaQO5PrY1a6_MxW4fWiH6uDXuu0C7H2R9XU56_3brd8zg7WHoKL1UFtwPoBtlWv8_w_8AftVhrA</recordid><startdate>198302</startdate><enddate>198302</enddate><creator>Graves, Peter N.</creator><creator>Weiss, Irwin K.</creator><creator>Perlish, Jerome S.</creator><creator>Fleischmajer, Raul</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198302</creationdate><title>Increased Procollagen mRNA Levels in Scleroderma Skin Fibroblasts</title><author>Graves, Peter N. ; Weiss, Irwin K. ; Perlish, Jerome S. ; Fleischmajer, Raul</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3408-a60624e5b4c13cd158bf81a4b410ab0f54d4cae7e5e1b056dd5e201563b8ebcc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Female</topic><topic>Fibroblasts - metabolism</topic><topic>Humans</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Procollagen - genetics</topic><topic>Protein Biosynthesis</topic><topic>Rabbits</topic><topic>Reticulocytes - metabolism</topic><topic>RNA, Messenger - genetics</topic><topic>Scleroderma, Systemic - metabolism</topic><topic>Skin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Graves, Peter N.</creatorcontrib><creatorcontrib>Weiss, Irwin K.</creatorcontrib><creatorcontrib>Perlish, Jerome S.</creatorcontrib><creatorcontrib>Fleischmajer, Raul</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of investigative dermatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Graves, Peter N.</au><au>Weiss, Irwin K.</au><au>Perlish, Jerome S.</au><au>Fleischmajer, Raul</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Increased Procollagen mRNA Levels in Scleroderma Skin Fibroblasts</atitle><jtitle>Journal of investigative dermatology</jtitle><addtitle>J Invest Dermatol</addtitle><date>1983-02</date><risdate>1983</risdate><volume>80</volume><issue>2</issue><spage>130</spage><epage>132</epage><pages>130-132</pages><issn>0022-202X</issn><eissn>1523-1747</eissn><abstract>Procollagen messenger RNA activity in scleroderma and normal skin fibroblasts was measured using a cell-free translation assay. Radioactive translation products were fractionated by electrophoresis and the ratio of procollagen to total incorporation was determined from densitometric scans of gel fluorograms. In 4 scleroderma cell lines 1.78% (± 0.10) of incorporated [35S]-methionine was in procollagen, compared to 1.00% (± 0.20) in 5 normal controls, These values are consistent with previously reported increases in the rates of collagen synthesis obtained with intact cells and show that most if not all of the increase can be explained on the basis of elevated translatable procollagen messenger RNA in scleroderma fibroblasts.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6822742</pmid><doi>10.1111/1523-1747.ep12532908</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-202X |
| ispartof | Journal of investigative dermatology, 1983-02, Vol.80 (2), p.130-132 |
| issn | 0022-202X 1523-1747 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Adult Animals Cells, Cultured Female Fibroblasts - metabolism Humans Male Middle Aged Procollagen - genetics Protein Biosynthesis Rabbits Reticulocytes - metabolism RNA, Messenger - genetics Scleroderma, Systemic - metabolism Skin - metabolism |
| title | Increased Procollagen mRNA Levels in Scleroderma Skin Fibroblasts |
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