Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil
Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by e...
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| Veröffentlicht in: | The Journal of hygiene 1983-02, Vol.90 (1), p.117-125 |
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| container_title | The Journal of hygiene |
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| creator | Pereira, H. G. Azeredo, Rosete S. Leite, J. P. G. Candeias, J. A. N. Rácz, Maria L. Linhares, A. C. Gabbay, Yvonne B. Trabulsi, J. R. |
| description | Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positi ve samples, 18 (13·0%) were classified as subgroup 1, 94 (68·1%) as subgroup 2, and 26 (18·8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretie pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories. |
| doi_str_mv | 10.1017/S0022172400063919 |
| format | Article |
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G. ; Azeredo, Rosete S. ; Leite, J. P. G. ; Candeias, J. A. N. ; Rácz, Maria L. ; Linhares, A. C. ; Gabbay, Yvonne B. ; Trabulsi, J. R.</creator><creatorcontrib>Pereira, H. G. ; Azeredo, Rosete S. ; Leite, J. P. G. ; Candeias, J. A. N. ; Rácz, Maria L. ; Linhares, A. C. ; Gabbay, Yvonne B. ; Trabulsi, J. R.</creatorcontrib><description>Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positi ve samples, 18 (13·0%) were classified as subgroup 1, 94 (68·1%) as subgroup 2, and 26 (18·8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretie pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. 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G.</creatorcontrib><creatorcontrib>Azeredo, Rosete S.</creatorcontrib><creatorcontrib>Leite, J. P. G.</creatorcontrib><creatorcontrib>Candeias, J. A. N.</creatorcontrib><creatorcontrib>Rácz, Maria L.</creatorcontrib><creatorcontrib>Linhares, A. C.</creatorcontrib><creatorcontrib>Gabbay, Yvonne B.</creatorcontrib><creatorcontrib>Trabulsi, J. R.</creatorcontrib><title>Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil</title><title>The Journal of hygiene</title><addtitle>J. Hyg</addtitle><description>Human rotaviruses from the states of Rio de Janeiro, São Paulo and Pará of Brazil were analysed by RNA electrophoresis. At least some bands characteristic of rotavirus double-stranded RNA were detected in 138 (86·8%) of 159 faecal samples in which the presence of rotavirus had been demonstrated by enzyme immunoassay. Of the RNA-positi ve samples, 18 (13·0%) were classified as subgroup 1, 94 (68·1%) as subgroup 2, and 26 (18·8%) could not be classified due to absence of visible bands 10 and 11. Subgroup 2 was more frequent in the three states. All strains of subgroup 1 detected in Rio de Janeiro were associated with a single short-lived school outbreak. All strains of subgroup 1 resembled each other in electrophoretie pattern, irrespective of geographical origin, although minor differences could be detected by co-electrophoresis. Subgroup 2, on the other hand, showed a great degree of electrophoretic heterogeneity and could be divided into several sub-categories.</description><subject>Brazil</subject><subject>Child</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzyme linked immunosorbent assay</subject><subject>Feces - microbiology</subject><subject>Gastroenteritis</subject><subject>Gastroenteritis - microbiology</subject><subject>Gels</subject><subject>Genes, Viral</subject><subject>Genomes</subject><subject>Humans</subject><subject>Microbiology</subject><subject>RNA</subject><subject>RNA, Double-Stranded - classification</subject><subject>RNA, Viral - classification</subject><subject>Rotavirus</subject><subject>Rotavirus - classification</subject><subject>Rotavirus - genetics</subject><subject>Rotavirus - isolation & purification</subject><subject>Rotavirus Infections - microbiology</subject><subject>Ungulates</subject><subject>Virology</subject><issn>0022-1724</issn><issn>2396-8184</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctuEzEUhi0EKiHwAEggecWqU-yxx5clLSVtVXFpy9pyxsfNhJlxas8g2rep-ih9sTpKlA0SrGzr-8_R53MQekvJASVUfrwkpCypLDkhRDBN9TM0KZkWhaKKP0eTNS7W_CV6ldKSkIpLyvbQnii1KEs1QcvjFuohhtUiRBiaGqdhdLc4eDwsAF9DHzpYvxZjZ3scw2B_N3FMkLCPocMXTcAO8JntoYlhH18-PgT83Y5twLZ3-RYf7_fxYbR3TfsavfC2TfBme07Rzy_HV0cnxfm32enRp_OirpgeCmYZaK1rYkUtlHdcOsqp95Za7SoNmjtbg6aeST13wD0IroQUpeLgNddsij5s-q5iuBkhDaZrUg1tmyXDmIwirFJSyP8GKasqKYTKQboJ1jGkFMGbVWw6G28NJWa9CPPXInLN-23zcd6B21VsJ5_5uw1fpiHEHWZKlPkzGRcb3KQB_uywjb9MNpeVEbMf5uvFydXZ7PNhNp0itlW03Tw27hrMMoyxz4P-h-QT4iSsHQ</recordid><startdate>198302</startdate><enddate>198302</enddate><creator>Pereira, H. 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| subjects | Brazil Child Electrophoresis Electrophoresis, Polyacrylamide Gel Enzyme linked immunosorbent assay Feces - microbiology Gastroenteritis Gastroenteritis - microbiology Gels Genes, Viral Genomes Humans Microbiology RNA RNA, Double-Stranded - classification RNA, Viral - classification Rotavirus Rotavirus - classification Rotavirus - genetics Rotavirus - isolation & purification Rotavirus Infections - microbiology Ungulates Virology |
| title | Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil |
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