Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies

We have developed an enzyme immunoassay (EL-ISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG 3 isotype was used as coating (antigen-capture) antibody, whi...

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Veröffentlicht in:	Transactions of the Royal Society of Tropical Medicine and Hygiene 1990-11, Vol.84 (6), p.815-818
Hauptverfasser:	De Jonge, N., Kremsner, P.G., Krijger, F.W., Schommer, G., Ffflié, Y.E., Kornelis, D., van Zeyl, R.J.M., Van Dam, G.J., Feldmeier, H., Deelder, A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal Antibody Specificity Antigens, Helminth - analysis Biological and medical sciences Biotin Diseases caused by trematodes Enzyme-Linked Immunosorbent Assay - methods Glycoproteins - analysis Helminth Proteins - analysis Helminthic diseases Humans Infectious diseases Medical sciences Parasitic diseases Schistosoma mansoni - immunology Schistosomiases Schistosomiasis mansoni - diagnosis Schistosomiasis mansoni - immunology Tropical medicine
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