Isolation of a vegetalizing inducing factor after extraction with acid ethanol. Concentration-dependent inducing capacity of the factor

A vegetalizing factor has been isolated from chicken embryos by an improved method. The factor is extracted with acid/ethanol and finally purified by four consecutive steps of reversed phase HPLC. The molecular mass is about 25 kDa. The factor dissociates after reduction with dithiothreitol into two...

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Veröffentlicht in:	Cell differentiation and development 1990-10, Vol.32 (1), p.27-38
Hauptverfasser:	Plessow, Sigrun, Davids, Michael, Born, Jochen, Hoppe, Peter, Loppnow-Blinde, Beate, Tiedemann, Heinz, Tiedemann, Hildegard
Format:	Artikel
Sprache:	eng
Schlagworte:	Acids Animals Biological Factors - isolation & purification Biological Factors - physiology Cell Differentiation - physiology Chick Embryo - chemistry Ectoderm - physiology Ethanol Gastrula - physiology Inducing capacity Purification from chicken embryo Vegetalizing inducing factor
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