Enzyme polymorphism in Trichinella
The isoenzyme profiles of five isolates of the supposed ‘species’ of Trichinella, T. nativa, T. spiralis and T. nelsoni were compared. Four enzymes (AK, PGM, MPI and GPI) gave good resolution and clearly differentiated T. spiralis from the other two species. T. nativa and T. nelsoni had similar isoe...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1982, Vol.76 (4), p.541-545 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| container_volume | 76 |
| creator | Flockhart, Hilary A. Harrison, Susan E. Dobinson, A.R. James, E.R. |
| description | The isoenzyme profiles of five isolates of the supposed ‘species’ of
Trichinella, T. nativa, T. spiralis and
T. nelsoni were compared. Four enzymes (AK, PGM, MPI and GPI) gave good resolution and clearly differentiated
T. spiralis from the other two species.
T. nativa and
T. nelsoni had similar isoenzyme patterns; the two separate isolates of
T. nativa and
T. spiralis used gave similar results, thus indicating the validity and the reproducibility of the technique. The value of enzyme electrophoresis for specific and subspecific classification of
Trichinella is discussed and compared with the more traditional methods of taxonomy which have failed to resolve the controversy surrounding speciation. |
| doi_str_mv | 10.1016/0035-9203(82)90159-6 |
| format | Article |
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Trichinella, T. nativa, T. spiralis and
T. nelsoni were compared. Four enzymes (AK, PGM, MPI and GPI) gave good resolution and clearly differentiated
T. spiralis from the other two species.
T. nativa and
T. nelsoni had similar isoenzyme patterns; the two separate isolates of
T. nativa and
T. spiralis used gave similar results, thus indicating the validity and the reproducibility of the technique. The value of enzyme electrophoresis for specific and subspecific classification of
Trichinella is discussed and compared with the more traditional methods of taxonomy which have failed to resolve the controversy surrounding speciation.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/0035-9203(82)90159-6</identifier><identifier>PMID: 6926776</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>disease diagnosis ; electrophoresis ; Electrophoresis, Starch Gel ; Enzymes, Nematoda ; Larva ; Nematoda ; nematode infections ; Polymorphism, Genetic ; Species Specificity ; taxonomy ; Trichinella - enzymology ; Trichinella nativa (Nematoda) ; Trichinella nelsoni (Nematoda) ; Trichinella spiralis ; variation</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 1982, Vol.76 (4), p.541-545</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c419t-978ed95625b3ac22ff84b9ff8856bc820b46dd64e749171917ab6fa6bd99cd983</citedby><cites>FETCH-LOGICAL-c419t-978ed95625b3ac22ff84b9ff8856bc820b46dd64e749171917ab6fa6bd99cd983</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,4012,27910,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6926776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Flockhart, Hilary A.</creatorcontrib><creatorcontrib>Harrison, Susan E.</creatorcontrib><creatorcontrib>Dobinson, A.R.</creatorcontrib><creatorcontrib>James, E.R.</creatorcontrib><title>Enzyme polymorphism in Trichinella</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>The isoenzyme profiles of five isolates of the supposed ‘species’ of
Trichinella, T. nativa, T. spiralis and
T. nelsoni were compared. Four enzymes (AK, PGM, MPI and GPI) gave good resolution and clearly differentiated
T. spiralis from the other two species.
T. nativa and
T. nelsoni had similar isoenzyme patterns; the two separate isolates of
T. nativa and
T. spiralis used gave similar results, thus indicating the validity and the reproducibility of the technique. The value of enzyme electrophoresis for specific and subspecific classification of
Trichinella is discussed and compared with the more traditional methods of taxonomy which have failed to resolve the controversy surrounding speciation.</description><subject>disease diagnosis</subject><subject>electrophoresis</subject><subject>Electrophoresis, Starch Gel</subject><subject>Enzymes, Nematoda</subject><subject>Larva</subject><subject>Nematoda</subject><subject>nematode infections</subject><subject>Polymorphism, Genetic</subject><subject>Species Specificity</subject><subject>taxonomy</subject><subject>Trichinella - enzymology</subject><subject>Trichinella nativa (Nematoda)</subject><subject>Trichinella nelsoni (Nematoda)</subject><subject>Trichinella spiralis</subject><subject>variation</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMoWj_-gWLxIHpYzcfm6yKIqBUFERWKlyGbzWq0u1uTVqy_3tQtPXqYmcP7zPDOi9AuwScEE3GKMeOZppgdKXqsMeE6EyuoR5RUGeOYraLeEtlAmzG-Y0x5wtbRutBUSCl66OCy-ZnVrj9uR7O6DeM3H-u-b_pPwds337jRyGyjtcqMottZzC30fHX5dDHI7u6vby7O7zKbEz3JtFSu1FxQXjBjKa0qlRc6dcVFYRXFRS7KUuRO5ppIksoUojKiKLW2pVZsCx12d8eh_Zy6OIHaRzt30Lh2GkFhRhlXPIF5B9rQxhhcBePgaxNmQDDMo4H53zD_GxSFv2hApLW9xf1pUbtyubTIIulZp_s4cd9L2YQPEJJJDoPhCzwQOhgO5S1cJX6_4yvTgnkNPsLzI8WEYSqo0jRPxFlHuBTbl3cBovWusa70wdkJlK3_3_IvvIaJrg</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Flockhart, Hilary A.</creator><creator>Harrison, Susan E.</creator><creator>Dobinson, A.R.</creator><creator>James, E.R.</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>Enzyme polymorphism in Trichinella</title><author>Flockhart, Hilary A. ; Harrison, Susan E. ; Dobinson, A.R. ; James, E.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c419t-978ed95625b3ac22ff84b9ff8856bc820b46dd64e749171917ab6fa6bd99cd983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>disease diagnosis</topic><topic>electrophoresis</topic><topic>Electrophoresis, Starch Gel</topic><topic>Enzymes, Nematoda</topic><topic>Larva</topic><topic>Nematoda</topic><topic>nematode infections</topic><topic>Polymorphism, Genetic</topic><topic>Species Specificity</topic><topic>taxonomy</topic><topic>Trichinella - enzymology</topic><topic>Trichinella nativa (Nematoda)</topic><topic>Trichinella nelsoni (Nematoda)</topic><topic>Trichinella spiralis</topic><topic>variation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Flockhart, Hilary A.</creatorcontrib><creatorcontrib>Harrison, Susan E.</creatorcontrib><creatorcontrib>Dobinson, A.R.</creatorcontrib><creatorcontrib>James, E.R.</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Flockhart, Hilary A.</au><au>Harrison, Susan E.</au><au>Dobinson, A.R.</au><au>James, E.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme polymorphism in Trichinella</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1982</date><risdate>1982</risdate><volume>76</volume><issue>4</issue><spage>541</spage><epage>545</epage><pages>541-545</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>The isoenzyme profiles of five isolates of the supposed ‘species’ of
Trichinella, T. nativa, T. spiralis and
T. nelsoni were compared. Four enzymes (AK, PGM, MPI and GPI) gave good resolution and clearly differentiated
T. spiralis from the other two species.
T. nativa and
T. nelsoni had similar isoenzyme patterns; the two separate isolates of
T. nativa and
T. spiralis used gave similar results, thus indicating the validity and the reproducibility of the technique. The value of enzyme electrophoresis for specific and subspecific classification of
Trichinella is discussed and compared with the more traditional methods of taxonomy which have failed to resolve the controversy surrounding speciation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6926776</pmid><doi>10.1016/0035-9203(82)90159-6</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1982, Vol.76 (4), p.541-545 |
| issn | 0035-9203 1878-3503 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80323585 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | disease diagnosis electrophoresis Electrophoresis, Starch Gel Enzymes, Nematoda Larva Nematoda nematode infections Polymorphism, Genetic Species Specificity taxonomy Trichinella - enzymology Trichinella nativa (Nematoda) Trichinella nelsoni (Nematoda) Trichinella spiralis variation |
| title | Enzyme polymorphism in Trichinella |
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