Purification of Thermus aquaticus DNA polymerase expressed in Escherichia coli

DNA polymerase from Thermus aquaticus has become a common reagent in molecular biology because of its utility in DNA amplification and DNA sequencing protocols. A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli. Purification requi...

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Veröffentlicht in:	Analytical biochemistry 1990-12, Vol.191 (2), p.396-400
Hauptverfasser:	Engelke, David R., Krikos, Alexandra, Bruck, Mary E., Ginsburg, David
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Chemical Precipitation Chromatography, Ion Exchange DNA-Directed DNA Polymerase - biosynthesis DNA-Directed DNA Polymerase - genetics DNA-Directed DNA Polymerase - isolation & purification Escherichia coli - enzymology Escherichia coli - genetics Molecular Sequence Data Plasmids Polyethyleneimine Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Taq Polymerase Thermus - enzymology Thermus - genetics
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