Morphologically and Functionally Distinct Subpopulations of Steroidogenic Cells in Corpora Lutea During Pregnancy in Rats
On days 5, 10, 15 and 20 of pregnancy, rat corpora lutea (CL) were dissected and dissociated into single cell suspensions by enzyme treatments. The suspended luteal cells were allowed to sediment in a BSA gradient at 4°C for 3.5 hours. Five fractions were collected from the top (Fraction (Fr.) 1) to...
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Veröffentlicht in: | Endocrinologia Japonica 1990, Vol.37(5), pp.649-663 |
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