In vitro proliferation and lifespan of bovine aorta endothelial cells: response to conditioned media
The effect of conditioned medium on the growth rate of bovine aorta endothelial cells (CSC311) was examined. Media conditioned by several cell types (both immortal and normal) was found to increase the growth rate of CSC311 cells. The growth rate of CSC311 was increased even when the medium was cond...
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| Veröffentlicht in: | Journal of cell science 1982-08, Vol.56 (1), p.281-292 |
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| container_title | Journal of cell science |
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| creator | Lincoln, D.W. II Whitney, R.G Smith, J.R |
| description | The effect of conditioned medium on the growth rate of bovine aorta endothelial cells (CSC311) was examined. Media conditioned by several cell types (both immortal and normal) was found to increase the growth rate of CSC311 cells. The growth rate of CSC311 was increased even when the medium was conditioned by other CSC311 cells. The rate of growth of unstimulated CSC311 cells is 0.43 population doublings (p.d./day. Conditioned medium from several cell types gave a maximum stimulated growth rate of 1.3 p.d./day. In addition, we present evidence for an increase in the degree of stimulation by the growth-promoting activity found in conditioned medium with increasing in vitro age of CSC311 cells. |
| doi_str_mv | 10.1242/jcs.56.1.281 |
| format | Article |
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II ; Whitney, R.G ; Smith, J.R</creator><creatorcontrib>Lincoln, D.W. II ; Whitney, R.G ; Smith, J.R</creatorcontrib><description>The effect of conditioned medium on the growth rate of bovine aorta endothelial cells (CSC311) was examined. Media conditioned by several cell types (both immortal and normal) was found to increase the growth rate of CSC311 cells. The growth rate of CSC311 was increased even when the medium was conditioned by other CSC311 cells. The rate of growth of unstimulated CSC311 cells is 0.43 population doublings (p.d./day. Conditioned medium from several cell types gave a maximum stimulated growth rate of 1.3 p.d./day. 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In addition, we present evidence for an increase in the degree of stimulation by the growth-promoting activity found in conditioned medium with increasing in vitro age of CSC311 cells.</description><subject>animal physiology</subject><subject>Animals</subject><subject>Aorta - cytology</subject><subject>Cattle</subject><subject>Cell Division</subject><subject>Cell Survival</subject><subject>Cells, Cultured</subject><subject>Culture Media</subject><subject>Endothelium - cytology</subject><subject>Growth Substances - physiology</subject><issn>0021-9533</issn><issn>1477-9137</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1PGzEQxS0EStPAjWuFT5y6qT_Xa24I0RYJiUPL2Zq1veBoYwd7E6n_PV4l6mVGo_fT07yH0DUla8oE-7GxZS3bNV2zjp6hJRVKNZpydY6WhDDaaMn5F_S1lA0hRDGtFmihaNvKtlsi9xTxIUw54V1OYxh8himkiCE6PJ9lBxGnAffpEKLHkPIE2EeXpnc_Bhix9eNY7nCuZIrF4ylhm6ILs4t3eOtdgEt0McBY_NVpr9Drz8e_D7-b55dfTw_3z41lUk9N65wXyvcAvZCaEyI60jnutO1tnXIQjLa2962HwepOcEKBK0615sqCZHyFbo--NcvH3pfJbEOZH4To076YjnAihZrB70fQ5lRK9oPZ5bCF_M9QYuZSTS3VyNZQU0ut-LeT776vgf7DpxarfnPUB0gG3nIo5vUPI7Q-qLWSgvFPqQ19Tg</recordid><startdate>198208</startdate><enddate>198208</enddate><creator>Lincoln, D.W. 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II ; Whitney, R.G ; Smith, J.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c259t-6dde47ebaab4593004808d3d9cbc3d95f4216cbe6eafc984301a37319937ca523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>animal physiology</topic><topic>Animals</topic><topic>Aorta - cytology</topic><topic>Cattle</topic><topic>Cell Division</topic><topic>Cell Survival</topic><topic>Cells, Cultured</topic><topic>Culture Media</topic><topic>Endothelium - cytology</topic><topic>Growth Substances - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lincoln, D.W. 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Conditioned medium from several cell types gave a maximum stimulated growth rate of 1.3 p.d./day. In addition, we present evidence for an increase in the degree of stimulation by the growth-promoting activity found in conditioned medium with increasing in vitro age of CSC311 cells.</abstract><cop>England</cop><pmid>7166568</pmid><doi>10.1242/jcs.56.1.281</doi><tpages>12</tpages></addata></record> |
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| ispartof | Journal of cell science, 1982-08, Vol.56 (1), p.281-292 |
| issn | 0021-9533 1477-9137 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Company of Biologists |
| subjects | animal physiology Animals Aorta - cytology Cattle Cell Division Cell Survival Cells, Cultured Culture Media Endothelium - cytology Growth Substances - physiology |
| title | In vitro proliferation and lifespan of bovine aorta endothelial cells: response to conditioned media |
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