Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures
A semi-automated cultivation apparatus for the in vitro culture of Plasmodium falciparum gametocytes is described. This apparatus has been designed to produce large numbers of fertile sexual stages for use in the development of a gamete vaccine or for the infection of suitable mosquitoes. These mosq...
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| Veröffentlicht in: | Transactions of the Royal Society of Tropical Medicine and Hygiene 1982, Vol.76 (6), p.812-818 |
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| container_title | Transactions of the Royal Society of Tropical Medicine and Hygiene |
| container_volume | 76 |
| creator | Ponnudurai, T. Lensen, A.H.W. Leeuwenberg, Anna D.E.M. Meuwissen, J.H.E.Th |
| description | A semi-automated cultivation apparatus for the
in vitro culture of
Plasmodium falciparum gametocytes is described. This apparatus has been designed to produce large numbers of fertile sexual stages for use in the development of a gamete vaccine or for the infection of suitable mosquitoes. These mosquitoes in turn may be used for the development of a possible sporozoite vaccine. Loss of red cells during medium change has been eliminated and the addition of warmed fresh medium simplified compared to similar systems described previously. Material harvested from this apparatus has been used for infecting mosquitoes. Up to 98% of
Anopheles stephensi were infected with a mean oocyst count of 24 per positive gut (range one to 109). The importance of satisfactory presentation of gametocytes for mosquito infection is stressed. The possible presence of substances in normal human sera which inhibits exflagellation to a variable degree and reduces mosquito infectivity is also discussed. |
| doi_str_mv | 10.1016/0035-9203(82)90116-X |
| format | Article |
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in vitro culture of
Plasmodium falciparum gametocytes is described. This apparatus has been designed to produce large numbers of fertile sexual stages for use in the development of a gamete vaccine or for the infection of suitable mosquitoes. These mosquitoes in turn may be used for the development of a possible sporozoite vaccine. Loss of red cells during medium change has been eliminated and the addition of warmed fresh medium simplified compared to similar systems described previously. Material harvested from this apparatus has been used for infecting mosquitoes. Up to 98% of
Anopheles stephensi were infected with a mean oocyst count of 24 per positive gut (range one to 109). The importance of satisfactory presentation of gametocytes for mosquito infection is stressed. The possible presence of substances in normal human sera which inhibits exflagellation to a variable degree and reduces mosquito infectivity is also discussed.</description><identifier>ISSN: 0035-9203</identifier><identifier>EISSN: 1878-3503</identifier><identifier>DOI: 10.1016/0035-9203(82)90116-X</identifier><identifier>PMID: 6761910</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Anopheles - parasitology ; Culture Media ; Electronics ; Equipment Design ; in vitro culture ; Parasitology - instrumentation ; Plasmodium falciparum ; Plasmodium falciparum - growth & development ; Protozoa</subject><ispartof>Transactions of the Royal Society of Tropical Medicine and Hygiene, 1982, Vol.76 (6), p.812-818</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-7a08bcf717d1f56f4d55d35a55601cc5396579d8aa6c35342e72fe11376665033</citedby><cites>FETCH-LOGICAL-c334t-7a08bcf717d1f56f4d55d35a55601cc5396579d8aa6c35342e72fe11376665033</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6761910$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ponnudurai, T.</creatorcontrib><creatorcontrib>Lensen, A.H.W.</creatorcontrib><creatorcontrib>Leeuwenberg, Anna D.E.M.</creatorcontrib><creatorcontrib>Meuwissen, J.H.E.Th</creatorcontrib><title>Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures</title><title>Transactions of the Royal Society of Tropical Medicine and Hygiene</title><addtitle>Trans R Soc Trop Med Hyg</addtitle><description>A semi-automated cultivation apparatus for the
in vitro culture of
Plasmodium falciparum gametocytes is described. This apparatus has been designed to produce large numbers of fertile sexual stages for use in the development of a gamete vaccine or for the infection of suitable mosquitoes. These mosquitoes in turn may be used for the development of a possible sporozoite vaccine. Loss of red cells during medium change has been eliminated and the addition of warmed fresh medium simplified compared to similar systems described previously. Material harvested from this apparatus has been used for infecting mosquitoes. Up to 98% of
Anopheles stephensi were infected with a mean oocyst count of 24 per positive gut (range one to 109). The importance of satisfactory presentation of gametocytes for mosquito infection is stressed. The possible presence of substances in normal human sera which inhibits exflagellation to a variable degree and reduces mosquito infectivity is also discussed.</description><subject>Anopheles - parasitology</subject><subject>Culture Media</subject><subject>Electronics</subject><subject>Equipment Design</subject><subject>in vitro culture</subject><subject>Parasitology - instrumentation</subject><subject>Plasmodium falciparum</subject><subject>Plasmodium falciparum - growth & development</subject><subject>Protozoa</subject><issn>0035-9203</issn><issn>1878-3503</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1vFCEUQInR1LX6DzTyZPRhVj4GZubFRDfWaqo2qTUbXwiFS4MOywpM4_57WWezjz5Bcs89gYPQU0qWlFD5mhAumoER_rJnrwZCqWzW99CC9l3fcEH4fbQ4Ig_Ro5x_EsIEFcMJOpGdpAMlC-RW01j8nS4-bnB02EEqfgR8OeocovVTwE6Pxm91qtdbHaBEsyuQsd_gDME3eiox6AIW510uEPIS0yW-KlVpsKn2KUF-jB5UTYYnh_MUXZ-9_7Y6by6-fvi4envRGM7b0nSa9DfGdbSz1AnpWiuE5UILIQk1RvBBim6wvdbScMFbBh1zQCnvpJT1y_wUvZi92xR_T5CLCj4bGEe9gThl1RM2VPsebGfQpJhzAqe2yQeddooStc-r9u3Uvp3qmfqXV63r2rODf7oJYI9Lh5513sxzX1P8OY51-lUR3gl1vv6h-JfP3_tPZ-_UZeWfz7zTUenb5LO6vmKEcsIk64dWVOLNTEDNduchqWw8bAxYn8AUZaP__5P_AiLPolo</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Ponnudurai, T.</creator><creator>Lensen, A.H.W.</creator><creator>Leeuwenberg, Anna D.E.M.</creator><creator>Meuwissen, J.H.E.Th</creator><general>Elsevier Ltd</general><general>Royal Society of Tropical Medicine and Hygiene</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures</title><author>Ponnudurai, T. ; Lensen, A.H.W. ; Leeuwenberg, Anna D.E.M. ; Meuwissen, J.H.E.Th</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-7a08bcf717d1f56f4d55d35a55601cc5396579d8aa6c35342e72fe11376665033</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Anopheles - parasitology</topic><topic>Culture Media</topic><topic>Electronics</topic><topic>Equipment Design</topic><topic>in vitro culture</topic><topic>Parasitology - instrumentation</topic><topic>Plasmodium falciparum</topic><topic>Plasmodium falciparum - growth & development</topic><topic>Protozoa</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ponnudurai, T.</creatorcontrib><creatorcontrib>Lensen, A.H.W.</creatorcontrib><creatorcontrib>Leeuwenberg, Anna D.E.M.</creatorcontrib><creatorcontrib>Meuwissen, J.H.E.Th</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ponnudurai, T.</au><au>Lensen, A.H.W.</au><au>Leeuwenberg, Anna D.E.M.</au><au>Meuwissen, J.H.E.Th</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures</atitle><jtitle>Transactions of the Royal Society of Tropical Medicine and Hygiene</jtitle><addtitle>Trans R Soc Trop Med Hyg</addtitle><date>1982</date><risdate>1982</risdate><volume>76</volume><issue>6</issue><spage>812</spage><epage>818</epage><pages>812-818</pages><issn>0035-9203</issn><eissn>1878-3503</eissn><abstract>A semi-automated cultivation apparatus for the
in vitro culture of
Plasmodium falciparum gametocytes is described. This apparatus has been designed to produce large numbers of fertile sexual stages for use in the development of a gamete vaccine or for the infection of suitable mosquitoes. These mosquitoes in turn may be used for the development of a possible sporozoite vaccine. Loss of red cells during medium change has been eliminated and the addition of warmed fresh medium simplified compared to similar systems described previously. Material harvested from this apparatus has been used for infecting mosquitoes. Up to 98% of
Anopheles stephensi were infected with a mean oocyst count of 24 per positive gut (range one to 109). The importance of satisfactory presentation of gametocytes for mosquito infection is stressed. The possible presence of substances in normal human sera which inhibits exflagellation to a variable degree and reduces mosquito infectivity is also discussed.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>6761910</pmid><doi>10.1016/0035-9203(82)90116-X</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0035-9203 |
| ispartof | Transactions of the Royal Society of Tropical Medicine and Hygiene, 1982, Vol.76 (6), p.812-818 |
| issn | 0035-9203 1878-3503 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80297173 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Anopheles - parasitology Culture Media Electronics Equipment Design in vitro culture Parasitology - instrumentation Plasmodium falciparum Plasmodium falciparum - growth & development Protozoa |
| title | Cultivation of fertile Plasmodium falciparum gametocytes in semi-automated systems. 1. Static cultures |
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