Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single‐strand binding protein
Summary Single‐stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 i...
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| Veröffentlicht in: | Molecular microbiology 1990-10, Vol.4 (10), p.1645-1652 |
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| container_title | Molecular microbiology |
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| creator | Carraway, M. Rewinski, C. Marinus, M. G. |
| description | Summary
Single‐stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates were deletions of apparently random length and substitutions (deletion/insertions). The intergenic deletions as well as the transition and frameshift mutations were identical to those previously isolated from mismatch repair‐defective cells in vivo. |
| doi_str_mv | 10.1111/j.1365-2958.1990.tb00541.x |
| format | Article |
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Single‐stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates were deletions of apparently random length and substitutions (deletion/insertions). The intergenic deletions as well as the transition and frameshift mutations were identical to those previously isolated from mismatch repair‐defective cells in vivo.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1990.tb00541.x</identifier><identifier>PMID: 1963919</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Base Sequence ; Biological and medical sciences ; Chromosome Deletion ; DNA Polymerase III - metabolism ; DNA Replication ; DNA Transposable Elements ; DNA, Bacterial - biosynthesis ; DNA, Bacterial - genetics ; DNA, Single-Stranded - biosynthesis ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli - metabolism ; Fundamental and applied biological sciences. Psychology ; Genes, Bacterial ; Genetics ; Microbiology ; Molecular Sequence Data ; Mutation ; Plasmids ; Templates, Genetic ; Tetracycline Resistance - genetics</subject><ispartof>Molecular microbiology, 1990-10, Vol.4 (10), p.1645-1652</ispartof><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3465-65f446d106da2758fc729575803aee3ba85a7644038e5d51a0bd88ce4cbb161a3</citedby><cites>FETCH-LOGICAL-c3465-65f446d106da2758fc729575803aee3ba85a7644038e5d51a0bd88ce4cbb161a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1990.tb00541.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1990.tb00541.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19442818$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1963919$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Carraway, M.</creatorcontrib><creatorcontrib>Rewinski, C.</creatorcontrib><creatorcontrib>Marinus, M. G.</creatorcontrib><title>Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single‐strand binding protein</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Single‐stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates were deletions of apparently random length and substitutions (deletion/insertions). The intergenic deletions as well as the transition and frameshift mutations were identical to those previously isolated from mismatch repair‐defective cells in vivo.</description><subject>Bacteriology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Deletion</subject><subject>DNA Polymerase III - metabolism</subject><subject>DNA Replication</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - biosynthesis</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Single-Stranded - biosynthesis</subject><subject>Escherichia coli</subject><subject>Escherichia coli - enzymology</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Bacterial</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Mutation</subject><subject>Plasmids</subject><subject>Templates, Genetic</subject><subject>Tetracycline Resistance - genetics</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUU2P0zAQtRBoKQs_AclCYm8NdvxRhwtaLQtE2sIFJG6W40yoq9QudgKbPe1PQPxEfgkOrYAbwpcZvXkzb8YPoSeUFDS_Z9uCMimWZSVUQauKFENDiOC0uL6DFr9Ld9GCVIIsmSo_3kcPUtoSQhmR7ASd0EqyilYL9H09DmZwwSe8j6EdLbS4mfDLt-d4H_ppB9EkwHVd403oA_ibDOHQ4ctkNxCd3TiDbegdNt0AETuPv7ghBpwmP2wguTRDOcOmSeDtr97k_Kceftx-S0M0Pus532ZoXmAA5x-ie53pEzw6xlP04dXl-4s3y6t3r-uL86ulZTyfKEXHuWwpka0pV0J1dpWvzglhBoA1RgmzkpwTpkC0ghrStEpZ4LZpqKSGnaKzw9ys-3mENOidSxb63ngIY9KK5Hl8tfonkcqSEFbyTHx-INoYUorQ6X10OxMnTYmendNbPdujZ3v07Jw-Oqevc_Pjo8rY7KD903qwKtefHusmWdN3-eusS3_ROC8VVZn34sD76nqY_mMDvV7XVHLBfgI9wLju</recordid><startdate>199010</startdate><enddate>199010</enddate><creator>Carraway, M.</creator><creator>Rewinski, C.</creator><creator>Marinus, M. G.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199010</creationdate><title>Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single‐strand binding protein</title><author>Carraway, M. ; Rewinski, C. ; Marinus, M. G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3465-65f446d106da2758fc729575803aee3ba85a7644038e5d51a0bd88ce4cbb161a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Bacteriology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Deletion</topic><topic>DNA Polymerase III - metabolism</topic><topic>DNA Replication</topic><topic>DNA Transposable Elements</topic><topic>DNA, Bacterial - biosynthesis</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Single-Stranded - biosynthesis</topic><topic>Escherichia coli</topic><topic>Escherichia coli - enzymology</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Bacterial</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Mutation</topic><topic>Plasmids</topic><topic>Templates, Genetic</topic><topic>Tetracycline Resistance - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carraway, M.</creatorcontrib><creatorcontrib>Rewinski, C.</creatorcontrib><creatorcontrib>Marinus, M. G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carraway, M.</au><au>Rewinski, C.</au><au>Marinus, M. G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single‐strand binding protein</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1990-10</date><risdate>1990</risdate><volume>4</volume><issue>10</issue><spage>1645</spage><epage>1652</epage><pages>1645-1652</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Single‐stranded plasmid DNA, containing the mnt gene, was replicated in vitro with DNA polymerase III holoenzyme. Escherichia colimutH bacteria, defective in mismatch repair, were transformed with the products of in vitro synthesis. Mutations in mnt were readily identified and 33 out of 65 isolates were single base changes including transition, transversion and frameshift mutations. The remaining 32 isolates were deletions of apparently random length and substitutions (deletion/insertions). The intergenic deletions as well as the transition and frameshift mutations were identical to those previously isolated from mismatch repair‐defective cells in vivo.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1963919</pmid><doi>10.1111/j.1365-2958.1990.tb00541.x</doi><tpages>8</tpages></addata></record> |
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| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1990-10, Vol.4 (10), p.1645-1652 |
| issn | 0950-382X 1365-2958 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Bacteriology Base Sequence Biological and medical sciences Chromosome Deletion DNA Polymerase III - metabolism DNA Replication DNA Transposable Elements DNA, Bacterial - biosynthesis DNA, Bacterial - genetics DNA, Single-Stranded - biosynthesis Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology Genes, Bacterial Genetics Microbiology Molecular Sequence Data Mutation Plasmids Templates, Genetic Tetracycline Resistance - genetics |
| title | Mutations produced by DNA polymerase III holoenzyme of Escherichia coli after in vitro synthesis in the absence of single‐strand binding protein |
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