The estimation of DNA contamination in RNA and enzyme preparations
Commercially available RNA and enzyme preparations are monitored for DNA contamination. The method used involves reaction with p-nitrophenylhydrazine and separation of the reaction products by thin-layer chromatography. When this technique is used, RNA preparations and bovine pancreas ribonuclease a...
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| Veröffentlicht in: | Analytical biochemistry 1982-11, Vol.127 (1), p.116-120 |
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| container_title | Analytical biochemistry |
| container_volume | 127 |
| creator | Radford, Ian R. Martin, Roger F. |
| description | Commercially available RNA and enzyme preparations are monitored for DNA contamination. The method used involves reaction with
p-nitrophenylhydrazine and separation of the reaction products by thin-layer chromatography. When this technique is used, RNA preparations and bovine pancreas ribonuclease are shown to have low levels of DNA contamination. An independent method, based on the enhanced fluorescence of DAPI in the presence of DNA, also detected DNA in the RNA preparations but not in the ribonuclease. |
| doi_str_mv | 10.1016/0003-2697(82)90153-1 |
| format | Article |
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p-nitrophenylhydrazine and separation of the reaction products by thin-layer chromatography. When this technique is used, RNA preparations and bovine pancreas ribonuclease are shown to have low levels of DNA contamination. An independent method, based on the enhanced fluorescence of DAPI in the presence of DNA, also detected DNA in the RNA preparations but not in the ribonuclease.</description><subject>DNA - isolation & purification</subject><subject>Drug Contamination</subject><subject>Enzymes - isolation & purification</subject><subject>Fluorescent Dyes</subject><subject>Indoles</subject><subject>RNA - isolation & purification</subject><subject>Spectrometry, Fluorescence</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKAzEUhoMotVbfQGFWoovRZJLJJBuh1isUBanrkMmcYKRzMZkK9elNndKlZ3Pg_P-5fQidEnxFMOHXGGOaZlwWFyK7lJjkNCV7aEyw5CmmWO6j8c5yiI5C-MSYEJbzERpxIoqMsjG6XXxAAqF3te5d2yStTe5epolpm17XrhmKrkneYlE3VQLNz7qGpPPQaf-nhmN0YPUywMk2T9D7w_1i9pTOXx-fZ9N5amhe9KkUWlbEZvF2yQwT0lKRxWwoJ5WkUBYWBNU5txh0mZUgLI9RMayZ1LKkE3Q-zO18-7WKN6vaBQPLpW6gXQUlcCYZxywa2WA0vg3Bg1Wdj__5tSJYbdCpDRe14aJEpv7QKRLbzrbzV2UN1a5pyyrqN4MO8clvB14F46AxUDkPpldV6_5f8AvjtXwz</recordid><startdate>19821115</startdate><enddate>19821115</enddate><creator>Radford, Ian R.</creator><creator>Martin, Roger F.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19821115</creationdate><title>The estimation of DNA contamination in RNA and enzyme preparations</title><author>Radford, Ian R. ; Martin, Roger F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-98a9d1f210194c489f382c48c361d93eb7fe83a56f0eab2be8f6666d40a49a9b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>DNA - isolation & purification</topic><topic>Drug Contamination</topic><topic>Enzymes - isolation & purification</topic><topic>Fluorescent Dyes</topic><topic>Indoles</topic><topic>RNA - isolation & purification</topic><topic>Spectrometry, Fluorescence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Radford, Ian R.</creatorcontrib><creatorcontrib>Martin, Roger F.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Radford, Ian R.</au><au>Martin, Roger F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The estimation of DNA contamination in RNA and enzyme preparations</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1982-11-15</date><risdate>1982</risdate><volume>127</volume><issue>1</issue><spage>116</spage><epage>120</epage><pages>116-120</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Commercially available RNA and enzyme preparations are monitored for DNA contamination. The method used involves reaction with
p-nitrophenylhydrazine and separation of the reaction products by thin-layer chromatography. When this technique is used, RNA preparations and bovine pancreas ribonuclease are shown to have low levels of DNA contamination. An independent method, based on the enhanced fluorescence of DAPI in the presence of DNA, also detected DNA in the RNA preparations but not in the ribonuclease.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>6187234</pmid><doi>10.1016/0003-2697(82)90153-1</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Analytical biochemistry, 1982-11, Vol.127 (1), p.116-120 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80294604 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | DNA - isolation & purification Drug Contamination Enzymes - isolation & purification Fluorescent Dyes Indoles RNA - isolation & purification Spectrometry, Fluorescence |
| title | The estimation of DNA contamination in RNA and enzyme preparations |
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