Column chromatography on polyethylenimine-silica: Rapid resolution of nucleotides and proteins with short columns and low pressures

Silica gel (100–200 mesh) can be coated with a stable layer of crosslinked polyethylenimine (PEI). The resulting material is useful in column chromatography for quantitative separation of adenine nucleotides. For example, 3′:5′-cyclic AMP can be separated from a mixture containing AMP, ADP, and ATP....

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Bibliographische Detailangaben

Veröffentlicht in:	Analytical biochemistry 1982-11, Vol.127 (1), p.155-158
Hauptverfasser:	Watanabe, K., Chow, W.-S., Royer, G.P.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, Ion Exchange - methods Nucleotides - analysis Polyethyleneimine Polyethylenes Pressure Proteins - analysis Silica Gel Silicon Dioxide
Online-Zugang:	Volltext
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