Proton NMR Microscopy of Multicellular Tumor Spheroid Morphology
We report proton NMR images obtained at microscopic (
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| Veröffentlicht in: | Magnetic resonance in medicine 1990-12, Vol.16 (3), p.380-389 |
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| container_title | Magnetic resonance in medicine |
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| creator | Sillerud, Laurel O. Freyer, James P. Neeman, Michal Mattingly, Mark A. |
| description | We report proton NMR images obtained at microscopic ( |
| doi_str_mv | 10.1002/j.1522-2594.1990.tb00001.x |
| format | Article |
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T1‐weighted images showed little contrast across a slice through the spheroid. There was also no difference between the inner and outer spheroid regions when signal intensity was measured as a function of the repitition time (TR), showing that T1 was the same across the spheroid. Conversely, T2‐weighted and multiecho images clearly revealed the central necrosis that occurs as the spheroids develop. Measurements of the thickness of the viable cell zone made on NMR images agreed with those made on standard histology sections for two different cell lines. The basis for the NMR discrimination of the necrotic region from the viable rim cells was found to be a shortened apparent T2 in the necrotic region (132 ± 17 ms) with respect to that in the viable cells (173 ± 9 ms). These results illustrate the applicability of NMR microscopy to assaying conditions inside intact tumor spheroids and suggest that this technology will allow the use of spheroids to investigate several important questions in tumor biology and pathophysiology.</description><identifier>ISSN: 0740-3194</identifier><identifier>EISSN: 1522-2594</identifier><identifier>DOI: 10.1002/j.1522-2594.1990.tb00001.x</identifier><identifier>PMID: 2077329</identifier><identifier>CODEN: MRMEEN</identifier><language>eng</language><publisher>Baltimore: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Biological and medical sciences ; Cell Line ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Magnetic Resonance Imaging ; Magnetic Resonance Spectroscopy ; Medical sciences ; Radiodiagnosis. Nmr imagery. 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T1‐weighted images showed little contrast across a slice through the spheroid. There was also no difference between the inner and outer spheroid regions when signal intensity was measured as a function of the repitition time (TR), showing that T1 was the same across the spheroid. Conversely, T2‐weighted and multiecho images clearly revealed the central necrosis that occurs as the spheroids develop. Measurements of the thickness of the viable cell zone made on NMR images agreed with those made on standard histology sections for two different cell lines. The basis for the NMR discrimination of the necrotic region from the viable rim cells was found to be a shortened apparent T2 in the necrotic region (132 ± 17 ms) with respect to that in the viable cells (173 ± 9 ms). These results illustrate the applicability of NMR microscopy to assaying conditions inside intact tumor spheroids and suggest that this technology will allow the use of spheroids to investigate several important questions in tumor biology and pathophysiology.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Humans</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Magnetic Resonance Imaging</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Medical sciences</subject><subject>Radiodiagnosis. Nmr imagery. Nmr spectrometry</subject><subject>Tumor Cells, Cultured</subject><issn>0740-3194</issn><issn>1522-2594</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkFtLwzAYhoMoc05_glBEvGvNqYd4pRueYFWZ8zqkSeo6uqUmLW7_3nYruzc3IbzPl7x5ALhCMEAQ4ttlgEKMfRwyGiDGYFBnsF0o2ByB4SE6BkMYU-gTxOgpOHNu2TKMxXQABhjGMcFsCO4_rKnN2ntLZ15aSGucNNXWM7mXNmVdSF2WTSmsN29Wxnqf1UJbUygvNbZamNJ8b8_BSS5Kpy_6fQS-nh7nkxd_-v78OnmY-hJThnyVxUyrKEGSEhVLhagUKmEJCSnJNFFE4ShEKqORUghLKHEWx4nQVIgkEjkmI3Czv7ey5qfRruarwnX1xFqbxvEEYobCiLbg3R7sPuOsznlli5WwW44g7_TxJe8c8c4R7_TxXh_ftMOX_StNttLqMNr7avPrPhdOijK3Yi0Ld8BCGuJk12G8x36LUm__UYCns3Q-3h3IH6n2jXI</recordid><startdate>199012</startdate><enddate>199012</enddate><creator>Sillerud, Laurel O.</creator><creator>Freyer, James P.</creator><creator>Neeman, Michal</creator><creator>Mattingly, Mark A.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199012</creationdate><title>Proton NMR Microscopy of Multicellular Tumor Spheroid Morphology</title><author>Sillerud, Laurel O. ; Freyer, James P. ; Neeman, Michal ; Mattingly, Mark A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2491-db79ed681c43d7cd14cad8983543be3d3d2651db46dd12c0c2b778ae4aa86af23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Magnetic Resonance Imaging</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Medical sciences</topic><topic>Radiodiagnosis. Nmr imagery. Nmr spectrometry</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sillerud, Laurel O.</creatorcontrib><creatorcontrib>Freyer, James P.</creatorcontrib><creatorcontrib>Neeman, Michal</creatorcontrib><creatorcontrib>Mattingly, Mark A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Magnetic resonance in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sillerud, Laurel O.</au><au>Freyer, James P.</au><au>Neeman, Michal</au><au>Mattingly, Mark A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proton NMR Microscopy of Multicellular Tumor Spheroid Morphology</atitle><jtitle>Magnetic resonance in medicine</jtitle><addtitle>Magn Reson Med</addtitle><date>1990-12</date><risdate>1990</risdate><volume>16</volume><issue>3</issue><spage>380</spage><epage>389</epage><pages>380-389</pages><issn>0740-3194</issn><eissn>1522-2594</eissn><coden>MRMEEN</coden><abstract>We report proton NMR images obtained at microscopic (<30 μm) resolution of EMT6/Ro and HT1080 multicellular tumor spheroids 1.2–1.7 mm in diameter. T1‐weighted images showed little contrast across a slice through the spheroid. There was also no difference between the inner and outer spheroid regions when signal intensity was measured as a function of the repitition time (TR), showing that T1 was the same across the spheroid. Conversely, T2‐weighted and multiecho images clearly revealed the central necrosis that occurs as the spheroids develop. Measurements of the thickness of the viable cell zone made on NMR images agreed with those made on standard histology sections for two different cell lines. The basis for the NMR discrimination of the necrotic region from the viable rim cells was found to be a shortened apparent T2 in the necrotic region (132 ± 17 ms) with respect to that in the viable cells (173 ± 9 ms). These results illustrate the applicability of NMR microscopy to assaying conditions inside intact tumor spheroids and suggest that this technology will allow the use of spheroids to investigate several important questions in tumor biology and pathophysiology.</abstract><cop>Baltimore</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>2077329</pmid><doi>10.1002/j.1522-2594.1990.tb00001.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | Magnetic resonance in medicine, 1990-12, Vol.16 (3), p.380-389 |
| issn | 0740-3194 1522-2594 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Biological and medical sciences Cell Line Humans Investigative techniques, diagnostic techniques (general aspects) Magnetic Resonance Imaging Magnetic Resonance Spectroscopy Medical sciences Radiodiagnosis. Nmr imagery. Nmr spectrometry Tumor Cells, Cultured |
| title | Proton NMR Microscopy of Multicellular Tumor Spheroid Morphology |
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