Purification of a membrane glycoprotein with an inositol-containing phospholipid anchor from Dictyostelium discoideum

Large-scale purification of a Dictyostelium discoideum cell surface glycoprotein, which is anchored in the membrane via a glycosylphosphatidylinositol (GPI) moiety, is described. The purification protocol involved four steps: separation of crude cell membranes by low-speed centrifugation, delipidiza...

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Veröffentlicht in:	Journal of biotechnology 1990-11, Vol.16 (3), p.233-243
Hauptverfasser:	Zhou-Chou, Ti, Gooley, Andrew A., Slade, Martin B., Bowers, Vivienne M., Williams, Keith L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibodies, Monoclonal antigens Biological and medical sciences Biotechnology Cell Fractionation Cell Membrane - chemistry cell surface Chromatography, Affinity Detergents Dictyostelium - analysis Dictyostelium - growth & development Dictyostelium discoideum Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology Fungal Proteins - chemistry Fungal Proteins - isolation & purification Glycolipids - physiology Glycosyl-phosphatidylinositol anchor glycosylphosphatidylinositol Glycosylphosphatidylinositols Immunoassay membrane glycoproteins Membrane Glycoproteins - chemistry Membrane Glycoproteins - isolation & purification Membrane protein purification Methods. Procedures. Technologies Others Phosphatidylinositols - physiology prespore-specific antigen Recombinant protein Recombinant Proteins - biosynthesis Recombinant Proteins - isolation & purification Thioglucosides Various methods and equipments
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