Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes
Erythroid cell-specific antisera capable of detecting tightly bound nonhistone chromatin protein--DNA complexes were obtained by injecting rabbits with dehistonized chicken reticulocyte chromatin. The antisera showed no crossreactivity with chromatin of thrombocytes which are regarded as cells genea...
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| Veröffentlicht in: | Molecular biology reports 1982-11, Vol.8 (4), p.199-202 |
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| creator | Krajewska, W M Klyszejko-Stefanowicz, L |
| description | Erythroid cell-specific antisera capable of detecting tightly bound nonhistone chromatin protein--DNA complexes were obtained by injecting rabbits with dehistonized chicken reticulocyte chromatin. The antisera showed no crossreactivity with chromatin of thrombocytes which are regarded as cells genealogically closely related with erythrocytes. The lack of thrombocyte chromatin immunoactivity was not caused by conformational constrains. Tightly bound nonhistone protein--DNA complexes isolated from thrombocyte chromatin showed no immunological similarity with these of erythrocyte chromatin. |
| doi_str_mv | 10.1007/BF00776580 |
| format | Article |
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The antisera showed no crossreactivity with chromatin of thrombocytes which are regarded as cells genealogically closely related with erythrocytes. The lack of thrombocyte chromatin immunoactivity was not caused by conformational constrains. 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Tightly bound nonhistone protein--DNA complexes isolated from thrombocyte chromatin showed no immunological similarity with these of erythrocyte chromatin.</description><subject>Animals</subject><subject>Antigen-Antibody Complex</subject><subject>Blood Platelets - analysis</subject><subject>Chickens</subject><subject>Chromosomal Proteins, Non-Histone - blood</subject><subject>Chromosomal Proteins, Non-Histone - immunology</subject><subject>Complement Fixation Tests</subject><subject>Deoxyribonucleoproteins - blood</subject><subject>Deoxyribonucleoproteins - immunology</subject><subject>Erythrocytes - analysis</subject><subject>Immune Sera</subject><subject>Nucleoproteins - immunology</subject><subject>Reticulocytes - analysis</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkT1PwzAQhi0EKqWwsCN5YkAKnBMndkYoFCpVsMAcOY5DDYldbGfIv8elFYwMdyfdPffqPhA6J3BNANjN3SJ6VuQcDtCU5CxLaMn4IZpCBiShPCfH6MT7DwCghOUTNGGkSHNCpsgt-34w1m-U1K2WOozYtthYs9Y-WKOwXDvbi6AN3jgblDYeB_2-Dt2IazuYBgeL759vcRuxCGv5qQwO26bayjEoj0WElBu3uZ_EKTpqRefV2T7O0Nvi4XX-lKxeHpfz21Ui05SHhAgh25LKRrRKZFByGQ1yYIKRuqRl3BYkb_NCUuBpQ3Ia64zRhqgG0rrIZuhypxsH_xqUD1WvvVRdJ4yyg684pLxgKf0XJFmRQcbLCF7tQOms90611cbpXrixIlBtP1H9fSLCF3vVoe5V84vuT599A9_FhKo</recordid><startdate>19821130</startdate><enddate>19821130</enddate><creator>Krajewska, W M</creator><creator>Klyszejko-Stefanowicz, L</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>19821130</creationdate><title>Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes</title><author>Krajewska, W M ; Klyszejko-Stefanowicz, L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c228t-1aacf94cdafea3098c0980507a71b9495800c8f56c4082d154098774d1ed02b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antigen-Antibody Complex</topic><topic>Blood Platelets - analysis</topic><topic>Chickens</topic><topic>Chromosomal Proteins, Non-Histone - blood</topic><topic>Chromosomal Proteins, Non-Histone - immunology</topic><topic>Complement Fixation Tests</topic><topic>Deoxyribonucleoproteins - blood</topic><topic>Deoxyribonucleoproteins - immunology</topic><topic>Erythrocytes - analysis</topic><topic>Immune Sera</topic><topic>Nucleoproteins - immunology</topic><topic>Reticulocytes - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Krajewska, W M</creatorcontrib><creatorcontrib>Klyszejko-Stefanowicz, L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Krajewska, W M</au><au>Klyszejko-Stefanowicz, L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes</atitle><jtitle>Molecular biology reports</jtitle><addtitle>Mol Biol Rep</addtitle><date>1982-11-30</date><risdate>1982</risdate><volume>8</volume><issue>4</issue><spage>199</spage><epage>202</epage><pages>199-202</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>Erythroid cell-specific antisera capable of detecting tightly bound nonhistone chromatin protein--DNA complexes were obtained by injecting rabbits with dehistonized chicken reticulocyte chromatin. 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| ispartof | Molecular biology reports, 1982-11, Vol.8 (4), p.199-202 |
| issn | 0301-4851 1573-4978 |
| language | eng |
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| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Animals Antigen-Antibody Complex Blood Platelets - analysis Chickens Chromosomal Proteins, Non-Histone - blood Chromosomal Proteins, Non-Histone - immunology Complement Fixation Tests Deoxyribonucleoproteins - blood Deoxyribonucleoproteins - immunology Erythrocytes - analysis Immune Sera Nucleoproteins - immunology Reticulocytes - analysis |
| title | Immunospecificity of nonhistone chromatin proteins tightly bound to DNA from chicken thrombocytes and erythrocytes |
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