Optimization of culture conditions for high cell density proliferation of HL-60 human promyelocytic leukemia cells

The purpose of the present investigation was to optimize the culture conditions in suspension of the HL-60 cell line for high-density production. The optimized HL medium was a mixture of RPMI-1640, DMEM, HamF12 and IMDM media supplemented with transferrin, insulin, Primatone RL, Pluronic F68, ethano...

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Veröffentlicht in:	Journal of cell science 1990-12, Vol.97 (4), p.639-647
Hauptverfasser:	SCHUMPP, B, SCHLAEGER, E.-J
Format:	Artikel
Sprache:	eng
Schlagworte:	Ammonia - metabolism Animal cells Biological and medical sciences Cell cultures. Hybridization. Fusion Cell Division Culture Media Dialysis Fundamental and applied biological sciences. Psychology Humans Kinetics Lactates - metabolism Leukemia, Promyelocytic, Acute Molecular and cellular biology Osmolar Concentration Perfusion Receptors, Cell Surface - metabolism Receptors, Tumor Necrosis Factor Tumor Cells, Cultured
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