A long-lived human diploid fibroblast line for cellular aging studies: Applications in cell hybridization
We have isolated a diploid fibroblast culture from human fetal lung with an in vitro lifespan of about 100 population doublings. The culture grows very well at clonal densities and long-lived clones can be isolated for use in cellular aging studies. The longer in vitro lifespan of the culture has al...
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| Veröffentlicht in: | Mechanisms of ageing and development 1982-01, Vol.20 (3), p.243-252 |
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| container_title | Mechanisms of ageing and development |
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| creator | Duthu, Gwen S. Braunschweiger, Karen I. Pereirasmith, Olivia M. Norwood, Thomas H. Smith, James R. |
| description | We have isolated a diploid fibroblast culture from human fetal lung with an
in vitro lifespan of about 100 population doublings. The culture grows very well at clonal densities and long-lived clones can be isolated for use in cellular aging studies. The longer
in vitro lifespan of the culture has allowed us to isolate from it a clone, containing a dominant and recessive mutation, having significant remaining proliferative potential. The nature of the mutations will allow for hybrid selection, after fusion of the mutant clone with wild type human cells. The mass culture and clones derived from it provide a valuable resource for cell aging studies. |
| doi_str_mv | 10.1016/0047-6374(82)90091-4 |
| format | Article |
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in vitro lifespan of about 100 population doublings. The culture grows very well at clonal densities and long-lived clones can be isolated for use in cellular aging studies. The longer
in vitro lifespan of the culture has allowed us to isolate from it a clone, containing a dominant and recessive mutation, having significant remaining proliferative potential. The nature of the mutations will allow for hybrid selection, after fusion of the mutant clone with wild type human cells. The mass culture and clones derived from it provide a valuable resource for cell aging studies.</description><identifier>ISSN: 0047-6374</identifier><identifier>EISSN: 1872-6216</identifier><identifier>DOI: 10.1016/0047-6374(82)90091-4</identifier><identifier>PMID: 7162221</identifier><language>eng</language><publisher>Ireland: Elsevier Ireland Ltd</publisher><subject>Aminopterin - pharmacology ; Cell Division - drug effects ; Cell Line ; Cell Survival - drug effects ; Clone Cells ; Diploidy ; Female ; Fetus ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Humans ; Hybridization, Genetic ; Hypoxanthines - metabolism ; Karyometry ; Lung - cytology ; Mutation ; Ouabain - pharmacology ; Pregnancy</subject><ispartof>Mechanisms of ageing and development, 1982-01, Vol.20 (3), p.243-252</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-11c7bc04f8108213e4c78f29ed2ea21f4181112a0d5af43da906c53fcceb5c13</citedby><cites>FETCH-LOGICAL-c452t-11c7bc04f8108213e4c78f29ed2ea21f4181112a0d5af43da906c53fcceb5c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0047-6374(82)90091-4$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7162221$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Duthu, Gwen S.</creatorcontrib><creatorcontrib>Braunschweiger, Karen I.</creatorcontrib><creatorcontrib>Pereirasmith, Olivia M.</creatorcontrib><creatorcontrib>Norwood, Thomas H.</creatorcontrib><creatorcontrib>Smith, James R.</creatorcontrib><title>A long-lived human diploid fibroblast line for cellular aging studies: Applications in cell hybridization</title><title>Mechanisms of ageing and development</title><addtitle>Mech Ageing Dev</addtitle><description>We have isolated a diploid fibroblast culture from human fetal lung with an
in vitro lifespan of about 100 population doublings. The culture grows very well at clonal densities and long-lived clones can be isolated for use in cellular aging studies. The longer
in vitro lifespan of the culture has allowed us to isolate from it a clone, containing a dominant and recessive mutation, having significant remaining proliferative potential. The nature of the mutations will allow for hybrid selection, after fusion of the mutant clone with wild type human cells. The mass culture and clones derived from it provide a valuable resource for cell aging studies.</description><subject>Aminopterin - pharmacology</subject><subject>Cell Division - drug effects</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Clone Cells</subject><subject>Diploidy</subject><subject>Female</subject><subject>Fetus</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Humans</subject><subject>Hybridization, Genetic</subject><subject>Hypoxanthines - metabolism</subject><subject>Karyometry</subject><subject>Lung - cytology</subject><subject>Mutation</subject><subject>Ouabain - pharmacology</subject><subject>Pregnancy</subject><issn>0047-6374</issn><issn>1872-6216</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE9r3DAQxUVJSTZpv0EDOoXm4FYja225h8ASkrQQ6CV3IUujzRSt5Up2IP303X_kmNPAzHtvZn6MfQHxDQQ034VQbdXUrfqq5XUnRAeV-sAWoFtZNRKaE7Z4k5yx81L-CCFAyeaUnbbQSClhwWjFYxrWVaQX9Px53tiBexpjIs8D9Tn10ZaJRxqQh5S5wxjnaDO3axrWvEyzJyw_-GocIzk7URoKp2Gv48-vfSZP__btT-xjsLHg52O9YE_3d0-3P6vH3w-_blePlVNLOVUAru2dUEGD0BJqVK7VQXboJVoJQYEGAGmFX9qgam870bhlHZzDfumgvmBXh9gxp78zlslsqOyusQOmuRgtpK472WyF6iB0OZWSMZgx08bmVwPC7ACbHT2zo2e0NHvARm1tl8f8ud-gfzMdiW7nN4c5bn98IcymOMLBoaeMbjI-0fsL_gM55YtI</recordid><startdate>19820101</startdate><enddate>19820101</enddate><creator>Duthu, Gwen S.</creator><creator>Braunschweiger, Karen I.</creator><creator>Pereirasmith, Olivia M.</creator><creator>Norwood, Thomas H.</creator><creator>Smith, James R.</creator><general>Elsevier Ireland Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820101</creationdate><title>A long-lived human diploid fibroblast line for cellular aging studies: Applications in cell hybridization</title><author>Duthu, Gwen S. ; Braunschweiger, Karen I. ; Pereirasmith, Olivia M. ; Norwood, Thomas H. ; Smith, James R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-11c7bc04f8108213e4c78f29ed2ea21f4181112a0d5af43da906c53fcceb5c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Aminopterin - pharmacology</topic><topic>Cell Division - drug effects</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Clone Cells</topic><topic>Diploidy</topic><topic>Female</topic><topic>Fetus</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Humans</topic><topic>Hybridization, Genetic</topic><topic>Hypoxanthines - metabolism</topic><topic>Karyometry</topic><topic>Lung - cytology</topic><topic>Mutation</topic><topic>Ouabain - pharmacology</topic><topic>Pregnancy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Duthu, Gwen S.</creatorcontrib><creatorcontrib>Braunschweiger, Karen I.</creatorcontrib><creatorcontrib>Pereirasmith, Olivia M.</creatorcontrib><creatorcontrib>Norwood, Thomas H.</creatorcontrib><creatorcontrib>Smith, James R.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mechanisms of ageing and development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Duthu, Gwen S.</au><au>Braunschweiger, Karen I.</au><au>Pereirasmith, Olivia M.</au><au>Norwood, Thomas H.</au><au>Smith, James R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A long-lived human diploid fibroblast line for cellular aging studies: Applications in cell hybridization</atitle><jtitle>Mechanisms of ageing and development</jtitle><addtitle>Mech Ageing Dev</addtitle><date>1982-01-01</date><risdate>1982</risdate><volume>20</volume><issue>3</issue><spage>243</spage><epage>252</epage><pages>243-252</pages><issn>0047-6374</issn><eissn>1872-6216</eissn><abstract>We have isolated a diploid fibroblast culture from human fetal lung with an
in vitro lifespan of about 100 population doublings. The culture grows very well at clonal densities and long-lived clones can be isolated for use in cellular aging studies. The longer
in vitro lifespan of the culture has allowed us to isolate from it a clone, containing a dominant and recessive mutation, having significant remaining proliferative potential. The nature of the mutations will allow for hybrid selection, after fusion of the mutant clone with wild type human cells. The mass culture and clones derived from it provide a valuable resource for cell aging studies.</abstract><cop>Ireland</cop><pub>Elsevier Ireland Ltd</pub><pmid>7162221</pmid><doi>10.1016/0047-6374(82)90091-4</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Mechanisms of ageing and development, 1982-01, Vol.20 (3), p.243-252 |
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| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Aminopterin - pharmacology Cell Division - drug effects Cell Line Cell Survival - drug effects Clone Cells Diploidy Female Fetus Fibroblasts - cytology Fibroblasts - drug effects Humans Hybridization, Genetic Hypoxanthines - metabolism Karyometry Lung - cytology Mutation Ouabain - pharmacology Pregnancy |
| title | A long-lived human diploid fibroblast line for cellular aging studies: Applications in cell hybridization |
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