Immune Response to Connective Tissue Components of the Basement Membrane

The immune response to connective tissue components of basement membrane (type IV collagen and laminin) and to interstitial collagen (type I) has been examined in human and murine systems. We also examined the role that immunologic sensitization to autologous connective tissue components might play...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Connective tissue research 1982, Vol.10 (3-4), p.333-343
Hauptverfasser: Mackel, Anne M., Delustro, Frank, Delustro, Barbara, Fudenberg, H. Hugh, Leroy, E. Carwile
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 343
container_issue 3-4
container_start_page 333
container_title Connective tissue research
container_volume 10
creator Mackel, Anne M.
Delustro, Frank
Delustro, Barbara
Fudenberg, H. Hugh
Leroy, E. Carwile
description The immune response to connective tissue components of basement membrane (type IV collagen and laminin) and to interstitial collagen (type I) has been examined in human and murine systems. We also examined the role that immunologic sensitization to autologous connective tissue components might play in inducing an inflammatory response resulting in pathologic sequelae. Mice receiving a single subcutaneous injection of 5 μg type IV or type I murine collagens, or murine laminin in complete Freund's adjuvant mount a delayed-type hyper-sensitivity response characterized by a mononuclear cell infiltrate when challenged in the footpad with the sensitizing antigen. Cell-mediated immunity to these connective tissue antigens can be transferred to normal syngeneic mice with sensitized T-lymphocytes. In addition, repeated immunizations with these homologous connective tissue components elicit antibody responses in mice. Our data demonstrate the immunogenic nature of types IV and I collagen, and of laminin in a syngeneic murine model. We have demonstrated autoantibodies to the basement membrane and interstitial collagens in the sera of patients with scleroderma (systemic sclerosis); ELISA ratios correlate directly with the extent of pulmonary fibrosis in these patients. Anti-type IV collagen autoantibodies were found to be primarily IgM and anti-type I collagen antibodies, primarily IgG. An antibody response to autologous connective tissue antigens could lead to complement activation, immune complex formation, and deposition of the complexes along vascular endothelium with recruitment of blood monocytes in situ, mirroring the early scleroderma lesion (perivascular mononuclear cell infiltrates). In vitro we examined the role of human peripheral blood mononuclear cells in the activation of fibroblasts. Adherent human blood monocytes release mediators which stimulate fibroblast proliferation and collagen deposition. A model is presented for the induction of immunity to autologous connective tissue components, leading to mononuclear cell inflammation, fibroblast activation and fibrosis. Selective immunity to basement membrane collagens may influence the clinical expression of diffuse connective tissue syndromes such as scleroderma (systemic sclerosis).
doi_str_mv 10.3109/03008208209008058
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_80280895</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>80280895</sourcerecordid><originalsourceid>FETCH-LOGICAL-c401t-d98f2c24e27c110eda5e50589260c6eb0c19db390fa6aaef0d00f50157064fcf3</originalsourceid><addsrcrecordid>eNp9kF9LwzAUxYMoc04_gA9Cn3yr3vRvir7oUDeYCDKfS5resI6mmUmq7NubsiGIKARuuOeew-FHyDmFq5hCcQ0xAIuGV_gPpOyAjGkGeciSmB2S8aCHXs2PyYm1awAax1E6IqMsoqzI4jGZzZXqOwxe0W50ZzFwOpjqrkPhmg8Mlo21PfqN8ip2zgZaBm6FwT23qPwieEZVGd7hKTmSvLV4tp8T8vb4sJzOwsXL03x6twhFAtSFdcFkJKIEo1xQCljzFFNfvIgyEBlWIGhRV3EBkmeco4QaQKZA0xyyRAoZT8jlLndj9HuP1pWqsQLb1nfQvS0ZRAxYkfpDujsURltrUJYb0yhutiWFcqBX_qLnPRf78L5SWH879ri8frvTm05qo_inNm1dOr5ttZGegmjsEP13_M0P-wp561aCGyzXujed5_ZPuS9nLI8B</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80280895</pqid></control><display><type>article</type><title>Immune Response to Connective Tissue Components of the Basement Membrane</title><source>MEDLINE</source><source>Taylor &amp; Francis Journals Complete</source><creator>Mackel, Anne M. ; Delustro, Frank ; Delustro, Barbara ; Fudenberg, H. Hugh ; Leroy, E. Carwile</creator><creatorcontrib>Mackel, Anne M. ; Delustro, Frank ; Delustro, Barbara ; Fudenberg, H. Hugh ; Leroy, E. Carwile</creatorcontrib><description>The immune response to connective tissue components of basement membrane (type IV collagen and laminin) and to interstitial collagen (type I) has been examined in human and murine systems. We also examined the role that immunologic sensitization to autologous connective tissue components might play in inducing an inflammatory response resulting in pathologic sequelae. Mice receiving a single subcutaneous injection of 5 μg type IV or type I murine collagens, or murine laminin in complete Freund's adjuvant mount a delayed-type hyper-sensitivity response characterized by a mononuclear cell infiltrate when challenged in the footpad with the sensitizing antigen. Cell-mediated immunity to these connective tissue antigens can be transferred to normal syngeneic mice with sensitized T-lymphocytes. In addition, repeated immunizations with these homologous connective tissue components elicit antibody responses in mice. Our data demonstrate the immunogenic nature of types IV and I collagen, and of laminin in a syngeneic murine model. We have demonstrated autoantibodies to the basement membrane and interstitial collagens in the sera of patients with scleroderma (systemic sclerosis); ELISA ratios correlate directly with the extent of pulmonary fibrosis in these patients. Anti-type IV collagen autoantibodies were found to be primarily IgM and anti-type I collagen antibodies, primarily IgG. An antibody response to autologous connective tissue antigens could lead to complement activation, immune complex formation, and deposition of the complexes along vascular endothelium with recruitment of blood monocytes in situ, mirroring the early scleroderma lesion (perivascular mononuclear cell infiltrates). In vitro we examined the role of human peripheral blood mononuclear cells in the activation of fibroblasts. Adherent human blood monocytes release mediators which stimulate fibroblast proliferation and collagen deposition. A model is presented for the induction of immunity to autologous connective tissue components, leading to mononuclear cell inflammation, fibroblast activation and fibrosis. Selective immunity to basement membrane collagens may influence the clinical expression of diffuse connective tissue syndromes such as scleroderma (systemic sclerosis).</description><identifier>ISSN: 0300-8207</identifier><identifier>EISSN: 1607-8438</identifier><identifier>DOI: 10.3109/03008208209008058</identifier><identifier>PMID: 6218963</identifier><language>eng</language><publisher>England: Informa UK Ltd</publisher><subject>Animals ; Antibody Formation ; Autoimmune Diseases - immunology ; Basement Membrane - immunology ; Collagen - immunology ; Connective Tissue - immunology ; Connective Tissue Diseases - immunology ; Fibroblasts - immunology ; Glycoproteins - immunology ; Humans ; Immunity, Cellular ; Immunoglobulin G - immunology ; Immunoglobulin M - immunology ; Inflammation - immunology ; Laminin ; Macrophages - immunology ; Major Histocompatibility Complex ; Mice ; Monocytes - immunology ; Scleroderma, Systemic - immunology</subject><ispartof>Connective tissue research, 1982, Vol.10 (3-4), p.333-343</ispartof><rights>1982 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c401t-d98f2c24e27c110eda5e50589260c6eb0c19db390fa6aaef0d00f50157064fcf3</citedby><cites>FETCH-LOGICAL-c401t-d98f2c24e27c110eda5e50589260c6eb0c19db390fa6aaef0d00f50157064fcf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/03008208209008058$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/03008208209008058$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,780,784,4024,27923,27924,27925,59647,60436,61221,61402</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6218963$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mackel, Anne M.</creatorcontrib><creatorcontrib>Delustro, Frank</creatorcontrib><creatorcontrib>Delustro, Barbara</creatorcontrib><creatorcontrib>Fudenberg, H. Hugh</creatorcontrib><creatorcontrib>Leroy, E. Carwile</creatorcontrib><title>Immune Response to Connective Tissue Components of the Basement Membrane</title><title>Connective tissue research</title><addtitle>Connect Tissue Res</addtitle><description>The immune response to connective tissue components of basement membrane (type IV collagen and laminin) and to interstitial collagen (type I) has been examined in human and murine systems. We also examined the role that immunologic sensitization to autologous connective tissue components might play in inducing an inflammatory response resulting in pathologic sequelae. Mice receiving a single subcutaneous injection of 5 μg type IV or type I murine collagens, or murine laminin in complete Freund's adjuvant mount a delayed-type hyper-sensitivity response characterized by a mononuclear cell infiltrate when challenged in the footpad with the sensitizing antigen. Cell-mediated immunity to these connective tissue antigens can be transferred to normal syngeneic mice with sensitized T-lymphocytes. In addition, repeated immunizations with these homologous connective tissue components elicit antibody responses in mice. Our data demonstrate the immunogenic nature of types IV and I collagen, and of laminin in a syngeneic murine model. We have demonstrated autoantibodies to the basement membrane and interstitial collagens in the sera of patients with scleroderma (systemic sclerosis); ELISA ratios correlate directly with the extent of pulmonary fibrosis in these patients. Anti-type IV collagen autoantibodies were found to be primarily IgM and anti-type I collagen antibodies, primarily IgG. An antibody response to autologous connective tissue antigens could lead to complement activation, immune complex formation, and deposition of the complexes along vascular endothelium with recruitment of blood monocytes in situ, mirroring the early scleroderma lesion (perivascular mononuclear cell infiltrates). In vitro we examined the role of human peripheral blood mononuclear cells in the activation of fibroblasts. Adherent human blood monocytes release mediators which stimulate fibroblast proliferation and collagen deposition. A model is presented for the induction of immunity to autologous connective tissue components, leading to mononuclear cell inflammation, fibroblast activation and fibrosis. Selective immunity to basement membrane collagens may influence the clinical expression of diffuse connective tissue syndromes such as scleroderma (systemic sclerosis).</description><subject>Animals</subject><subject>Antibody Formation</subject><subject>Autoimmune Diseases - immunology</subject><subject>Basement Membrane - immunology</subject><subject>Collagen - immunology</subject><subject>Connective Tissue - immunology</subject><subject>Connective Tissue Diseases - immunology</subject><subject>Fibroblasts - immunology</subject><subject>Glycoproteins - immunology</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunoglobulin M - immunology</subject><subject>Inflammation - immunology</subject><subject>Laminin</subject><subject>Macrophages - immunology</subject><subject>Major Histocompatibility Complex</subject><subject>Mice</subject><subject>Monocytes - immunology</subject><subject>Scleroderma, Systemic - immunology</subject><issn>0300-8207</issn><issn>1607-8438</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF9LwzAUxYMoc04_gA9Cn3yr3vRvir7oUDeYCDKfS5resI6mmUmq7NubsiGIKARuuOeew-FHyDmFq5hCcQ0xAIuGV_gPpOyAjGkGeciSmB2S8aCHXs2PyYm1awAax1E6IqMsoqzI4jGZzZXqOwxe0W50ZzFwOpjqrkPhmg8Mlo21PfqN8ip2zgZaBm6FwT23qPwieEZVGd7hKTmSvLV4tp8T8vb4sJzOwsXL03x6twhFAtSFdcFkJKIEo1xQCljzFFNfvIgyEBlWIGhRV3EBkmeco4QaQKZA0xyyRAoZT8jlLndj9HuP1pWqsQLb1nfQvS0ZRAxYkfpDujsURltrUJYb0yhutiWFcqBX_qLnPRf78L5SWH879ri8frvTm05qo_inNm1dOr5ttZGegmjsEP13_M0P-wp561aCGyzXujed5_ZPuS9nLI8B</recordid><startdate>1982</startdate><enddate>1982</enddate><creator>Mackel, Anne M.</creator><creator>Delustro, Frank</creator><creator>Delustro, Barbara</creator><creator>Fudenberg, H. Hugh</creator><creator>Leroy, E. Carwile</creator><general>Informa UK Ltd</general><general>Taylor &amp; Francis</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1982</creationdate><title>Immune Response to Connective Tissue Components of the Basement Membrane</title><author>Mackel, Anne M. ; Delustro, Frank ; Delustro, Barbara ; Fudenberg, H. Hugh ; Leroy, E. Carwile</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c401t-d98f2c24e27c110eda5e50589260c6eb0c19db390fa6aaef0d00f50157064fcf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antibody Formation</topic><topic>Autoimmune Diseases - immunology</topic><topic>Basement Membrane - immunology</topic><topic>Collagen - immunology</topic><topic>Connective Tissue - immunology</topic><topic>Connective Tissue Diseases - immunology</topic><topic>Fibroblasts - immunology</topic><topic>Glycoproteins - immunology</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Immunoglobulin G - immunology</topic><topic>Immunoglobulin M - immunology</topic><topic>Inflammation - immunology</topic><topic>Laminin</topic><topic>Macrophages - immunology</topic><topic>Major Histocompatibility Complex</topic><topic>Mice</topic><topic>Monocytes - immunology</topic><topic>Scleroderma, Systemic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mackel, Anne M.</creatorcontrib><creatorcontrib>Delustro, Frank</creatorcontrib><creatorcontrib>Delustro, Barbara</creatorcontrib><creatorcontrib>Fudenberg, H. Hugh</creatorcontrib><creatorcontrib>Leroy, E. Carwile</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Connective tissue research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mackel, Anne M.</au><au>Delustro, Frank</au><au>Delustro, Barbara</au><au>Fudenberg, H. Hugh</au><au>Leroy, E. Carwile</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immune Response to Connective Tissue Components of the Basement Membrane</atitle><jtitle>Connective tissue research</jtitle><addtitle>Connect Tissue Res</addtitle><date>1982</date><risdate>1982</risdate><volume>10</volume><issue>3-4</issue><spage>333</spage><epage>343</epage><pages>333-343</pages><issn>0300-8207</issn><eissn>1607-8438</eissn><abstract>The immune response to connective tissue components of basement membrane (type IV collagen and laminin) and to interstitial collagen (type I) has been examined in human and murine systems. We also examined the role that immunologic sensitization to autologous connective tissue components might play in inducing an inflammatory response resulting in pathologic sequelae. Mice receiving a single subcutaneous injection of 5 μg type IV or type I murine collagens, or murine laminin in complete Freund's adjuvant mount a delayed-type hyper-sensitivity response characterized by a mononuclear cell infiltrate when challenged in the footpad with the sensitizing antigen. Cell-mediated immunity to these connective tissue antigens can be transferred to normal syngeneic mice with sensitized T-lymphocytes. In addition, repeated immunizations with these homologous connective tissue components elicit antibody responses in mice. Our data demonstrate the immunogenic nature of types IV and I collagen, and of laminin in a syngeneic murine model. We have demonstrated autoantibodies to the basement membrane and interstitial collagens in the sera of patients with scleroderma (systemic sclerosis); ELISA ratios correlate directly with the extent of pulmonary fibrosis in these patients. Anti-type IV collagen autoantibodies were found to be primarily IgM and anti-type I collagen antibodies, primarily IgG. An antibody response to autologous connective tissue antigens could lead to complement activation, immune complex formation, and deposition of the complexes along vascular endothelium with recruitment of blood monocytes in situ, mirroring the early scleroderma lesion (perivascular mononuclear cell infiltrates). In vitro we examined the role of human peripheral blood mononuclear cells in the activation of fibroblasts. Adherent human blood monocytes release mediators which stimulate fibroblast proliferation and collagen deposition. A model is presented for the induction of immunity to autologous connective tissue components, leading to mononuclear cell inflammation, fibroblast activation and fibrosis. Selective immunity to basement membrane collagens may influence the clinical expression of diffuse connective tissue syndromes such as scleroderma (systemic sclerosis).</abstract><cop>England</cop><pub>Informa UK Ltd</pub><pmid>6218963</pmid><doi>10.3109/03008208209008058</doi><tpages>11</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0300-8207
ispartof Connective tissue research, 1982, Vol.10 (3-4), p.333-343
issn 0300-8207
1607-8438
language eng
recordid cdi_proquest_miscellaneous_80280895
source MEDLINE; Taylor & Francis Journals Complete
subjects Animals
Antibody Formation
Autoimmune Diseases - immunology
Basement Membrane - immunology
Collagen - immunology
Connective Tissue - immunology
Connective Tissue Diseases - immunology
Fibroblasts - immunology
Glycoproteins - immunology
Humans
Immunity, Cellular
Immunoglobulin G - immunology
Immunoglobulin M - immunology
Inflammation - immunology
Laminin
Macrophages - immunology
Major Histocompatibility Complex
Mice
Monocytes - immunology
Scleroderma, Systemic - immunology
title Immune Response to Connective Tissue Components of the Basement Membrane
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T08%3A38%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Immune%20Response%20to%20Connective%20Tissue%20Components%20of%20the%20Basement%20Membrane&rft.jtitle=Connective%20tissue%20research&rft.au=Mackel,%20Anne%20M.&rft.date=1982&rft.volume=10&rft.issue=3-4&rft.spage=333&rft.epage=343&rft.pages=333-343&rft.issn=0300-8207&rft.eissn=1607-8438&rft_id=info:doi/10.3109/03008208209008058&rft_dat=%3Cproquest_cross%3E80280895%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80280895&rft_id=info:pmid/6218963&rfr_iscdi=true