Anti-substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro
Anti-idiotypic antibodies (anti-Id) obtained in rabbits in response to immunization with polyclonal anti-substance P antibodies (anti-SP) were shown to bind specifically and with high affinity to membranes from rat parotid gland cells. Whereas substace P (SP) was unable to displace anti-Id from memb...
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| Veröffentlicht in: | European journal of pharmacology 1990-10, Vol.187 (3), p.357-367 |
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| creator | Maillet, Sylvie Imhoff, Viviane Rossignol, Bernard Grassi, Jacques Pradelles, Philippe Couraud, Jean-Yves |
| description | Anti-idiotypic antibodies (anti-Id) obtained in rabbits in response to immunization with polyclonal anti-substance P antibodies (anti-SP) were shown to bind specifically and with high affinity to membranes from rat parotid gland cells. Whereas substace P (SP) was unable to displace anti-Id from membrane binding sites, anti-Id partly inhibited the binding of radiolabelled substance P. Like substance P, anti-Id were able to trigger protein secretion by parotid cells, i.e. to behave as physiological agonists of the neuropeptide. Under our experimental conditions, the biological effects of both ligands appear to be additive. Unlike substance P, however, anti-Id dir not potentiate the secretory responses induced by a β-adrenoceptor agonist. Taken together, the present results might indicate that anti-Id interact with epitope(s) at or/and near he peptide-combining site on the substance P receptor. These data demonstrate further the possibility of raising pharmacologically active anti-receptor antibodies through the immunological anti-idiotypic approach. |
| doi_str_mv | 10.1016/0014-2999(90)90363-B |
| format | Article |
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Whereas substace P (SP) was unable to displace anti-Id from membrane binding sites, anti-Id partly inhibited the binding of radiolabelled substance P. Like substance P, anti-Id were able to trigger protein secretion by parotid cells, i.e. to behave as physiological agonists of the neuropeptide. Under our experimental conditions, the biological effects of both ligands appear to be additive. Unlike substance P, however, anti-Id dir not potentiate the secretory responses induced by a β-adrenoceptor agonist. Taken together, the present results might indicate that anti-Id interact with epitope(s) at or/and near he peptide-combining site on the substance P receptor. These data demonstrate further the possibility of raising pharmacologically active anti-receptor antibodies through the immunological anti-idiotypic approach.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(90)90363-B</identifier><identifier>PMID: 1705891</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Anti-idiotypic antibodies ; Antibodies, Anti-Idiotypic - isolation & purification ; Antibodies, Anti-Idiotypic - metabolism ; Antibodies, Monoclonal ; Biological and medical sciences ; Cyclic AMP - metabolism ; Fundamental and applied biological sciences. Psychology ; Immunoglobulins - isolation & purification ; In Vitro Techniques ; Iodine Radioisotopes ; Isoproterenol - pharmacology ; Male ; Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus ; Parotid gland (rat) ; Parotid Gland - immunology ; Parotid Gland - metabolism ; Protein secretion ; Rats ; Rats, Inbred Strains ; Receptors, Neurokinin-1 ; Receptors, Neurotransmitter - immunology ; Receptors, Neurotransmitter - metabolism ; Substance P - immunology ; Substance P receptors ; Vertebrates: digestive system</subject><ispartof>European journal of pharmacology, 1990-10, Vol.187 (3), p.357-367</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c302t-60ce1ddd6463362a3e862222bb4a23f68355d9472909421fc17220d70cd018613</citedby><cites>FETCH-LOGICAL-c302t-60ce1ddd6463362a3e862222bb4a23f68355d9472909421fc17220d70cd018613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(90)90363-B$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19386470$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1705891$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maillet, Sylvie</creatorcontrib><creatorcontrib>Imhoff, Viviane</creatorcontrib><creatorcontrib>Rossignol, Bernard</creatorcontrib><creatorcontrib>Grassi, Jacques</creatorcontrib><creatorcontrib>Pradelles, Philippe</creatorcontrib><creatorcontrib>Couraud, Jean-Yves</creatorcontrib><title>Anti-substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Anti-idiotypic antibodies (anti-Id) obtained in rabbits in response to immunization with polyclonal anti-substance P antibodies (anti-SP) were shown to bind specifically and with high affinity to membranes from rat parotid gland cells. Whereas substace P (SP) was unable to displace anti-Id from membrane binding sites, anti-Id partly inhibited the binding of radiolabelled substance P. Like substance P, anti-Id were able to trigger protein secretion by parotid cells, i.e. to behave as physiological agonists of the neuropeptide. Under our experimental conditions, the biological effects of both ligands appear to be additive. Unlike substance P, however, anti-Id dir not potentiate the secretory responses induced by a β-adrenoceptor agonist. Taken together, the present results might indicate that anti-Id interact with epitope(s) at or/and near he peptide-combining site on the substance P receptor. These data demonstrate further the possibility of raising pharmacologically active anti-receptor antibodies through the immunological anti-idiotypic approach.</description><subject>Animals</subject><subject>Anti-idiotypic antibodies</subject><subject>Antibodies, Anti-Idiotypic - isolation & purification</subject><subject>Antibodies, Anti-Idiotypic - metabolism</subject><subject>Antibodies, Monoclonal</subject><subject>Biological and medical sciences</subject><subject>Cyclic AMP - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Immunoglobulins - isolation & purification</subject><subject>In Vitro Techniques</subject><subject>Iodine Radioisotopes</subject><subject>Isoproterenol - pharmacology</subject><subject>Male</subject><subject>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</subject><subject>Parotid gland (rat)</subject><subject>Parotid Gland - immunology</subject><subject>Parotid Gland - metabolism</subject><subject>Protein secretion</subject><subject>Rats</subject><subject>Rats, Inbred Strains</subject><subject>Receptors, Neurokinin-1</subject><subject>Receptors, Neurotransmitter - immunology</subject><subject>Receptors, Neurotransmitter - metabolism</subject><subject>Substance P - immunology</subject><subject>Substance P receptors</subject><subject>Vertebrates: digestive system</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFvFCEUx4nR1LX1G2jCRaOHqQ-YYeDSpG1aNWlSD_VMGHij6OyyAtNkv73M7sbe5EJ4_997eflByBsG5wyY_ATA2oZrrT9o-KhBSNFcPSMrpnrdQM_4c7L6h7wkr3L-BQCd5t0JOWE9dEqzFfl9uSmhyfOQi904pN-oXQrBh1h22-D2zyH6gJmuo58nW5CWn0gzuoQlph3dpugwZxo2-yDZQrc2xRI8_THZjV-Cx1BSPCMvRjtlfH28T8n325uH6y_N3f3nr9eXd40TwEsjwSHz3stWCiG5Fagkr2cYWsvFKJXoOq_bnmvQLWejYz3n4HtwHpiSTJyS94e5dbM_M-Zi1iE7nOoyGOdsFPBeKaYr2B5Al2LOCUezTWFt084wMItjswg0i0Cjwewdm6va9vY4fx7W6J-aDlJr_u6Y2-zsNKZqNuQnTAsl2x4qd3HgsMp4DJhMdgHrL_iQ0BXjY_j_In8BWqaX_w</recordid><startdate>19901023</startdate><enddate>19901023</enddate><creator>Maillet, Sylvie</creator><creator>Imhoff, Viviane</creator><creator>Rossignol, Bernard</creator><creator>Grassi, Jacques</creator><creator>Pradelles, Philippe</creator><creator>Couraud, Jean-Yves</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19901023</creationdate><title>Anti-substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro</title><author>Maillet, Sylvie ; Imhoff, Viviane ; Rossignol, Bernard ; Grassi, Jacques ; Pradelles, Philippe ; Couraud, Jean-Yves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c302t-60ce1ddd6463362a3e862222bb4a23f68355d9472909421fc17220d70cd018613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Anti-idiotypic antibodies</topic><topic>Antibodies, Anti-Idiotypic - isolation & purification</topic><topic>Antibodies, Anti-Idiotypic - metabolism</topic><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Cyclic AMP - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Immunoglobulins - isolation & purification</topic><topic>In Vitro Techniques</topic><topic>Iodine Radioisotopes</topic><topic>Isoproterenol - pharmacology</topic><topic>Male</topic><topic>Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus</topic><topic>Parotid gland (rat)</topic><topic>Parotid Gland - immunology</topic><topic>Parotid Gland - metabolism</topic><topic>Protein secretion</topic><topic>Rats</topic><topic>Rats, Inbred Strains</topic><topic>Receptors, Neurokinin-1</topic><topic>Receptors, Neurotransmitter - immunology</topic><topic>Receptors, Neurotransmitter - metabolism</topic><topic>Substance P - immunology</topic><topic>Substance P receptors</topic><topic>Vertebrates: digestive system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maillet, Sylvie</creatorcontrib><creatorcontrib>Imhoff, Viviane</creatorcontrib><creatorcontrib>Rossignol, Bernard</creatorcontrib><creatorcontrib>Grassi, Jacques</creatorcontrib><creatorcontrib>Pradelles, Philippe</creatorcontrib><creatorcontrib>Couraud, Jean-Yves</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maillet, Sylvie</au><au>Imhoff, Viviane</au><au>Rossignol, Bernard</au><au>Grassi, Jacques</au><au>Pradelles, Philippe</au><au>Couraud, Jean-Yves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1990-10-23</date><risdate>1990</risdate><volume>187</volume><issue>3</issue><spage>357</spage><epage>367</epage><pages>357-367</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Anti-idiotypic antibodies (anti-Id) obtained in rabbits in response to immunization with polyclonal anti-substance P antibodies (anti-SP) were shown to bind specifically and with high affinity to membranes from rat parotid gland cells. Whereas substace P (SP) was unable to displace anti-Id from membrane binding sites, anti-Id partly inhibited the binding of radiolabelled substance P. Like substance P, anti-Id were able to trigger protein secretion by parotid cells, i.e. to behave as physiological agonists of the neuropeptide. Under our experimental conditions, the biological effects of both ligands appear to be additive. Unlike substance P, however, anti-Id dir not potentiate the secretory responses induced by a β-adrenoceptor agonist. Taken together, the present results might indicate that anti-Id interact with epitope(s) at or/and near he peptide-combining site on the substance P receptor. These data demonstrate further the possibility of raising pharmacologically active anti-receptor antibodies through the immunological anti-idiotypic approach.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1705891</pmid><doi>10.1016/0014-2999(90)90363-B</doi><tpages>11</tpages></addata></record> |
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| subjects | Animals Anti-idiotypic antibodies Antibodies, Anti-Idiotypic - isolation & purification Antibodies, Anti-Idiotypic - metabolism Antibodies, Monoclonal Biological and medical sciences Cyclic AMP - metabolism Fundamental and applied biological sciences. Psychology Immunoglobulins - isolation & purification In Vitro Techniques Iodine Radioisotopes Isoproterenol - pharmacology Male Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus Parotid gland (rat) Parotid Gland - immunology Parotid Gland - metabolism Protein secretion Rats Rats, Inbred Strains Receptors, Neurokinin-1 Receptors, Neurotransmitter - immunology Receptors, Neurotransmitter - metabolism Substance P - immunology Substance P receptors Vertebrates: digestive system |
| title | Anti-substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro |
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