[4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes
This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits. General methods for loading the dyes into cells, attaching the glands to coverslips and perfusing them with different s...
Gespeichert in:
| Veröffentlicht in: | Methods in Enzymology 1990, Vol.192, p.38-81 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 81 |
|---|---|
| container_issue | |
| container_start_page | 38 |
| container_title | Methods in Enzymology |
| container_volume | 192 |
| creator | Negulescu, Paul A. Machen, Terry E. |
| description | This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits. General methods for loading the dyes into cells, attaching the glands to coverslips and perfusing them with different solutions using flow-through chambers will be described. Techniques for assessing intracellular dye behavior and calibrating the dye fluorescence in cells will be discussed. In addition, approaches for identifying specific membrane transport systems and for measuring rates of transport by these mechanisms will be presented. One advantage of using fluorescent probes is that the same rationing system can be used for measuring any of the dyes discussed in this chapter. This is particularly advantageous because there are many potential interactions among the various ions and ion transporters. With such considerations as guidelines, fluorescent ion indicators should continue to provide unique insights into cellular physiology for many years. |
| doi_str_mv | 10.1016/0076-6879(90)92062-I |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_80276642</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>007668799092062I</els_id><sourcerecordid>80276642</sourcerecordid><originalsourceid>FETCH-LOGICAL-c393t-30f26604cd9ae7d7f567cfc4d12373bd861dd1a479e563a346341eecb23c2a803</originalsourceid><addsrcrecordid>eNo9kUlrHDEQhYVjMxkv_8AGXWLiQydaurVcDMEk8cCAL8kpBKGRqrFML2NJPcb_3mp7mLrUob4q3quH0CUl3yih4jshUlRCSf1VkxvNiGDV6ggtadPISmqlPqFTQhlVjGiuj9HywH9GFyk9kVKNqFVDF2jBiKyl1kv0-K_-j1dDjtZB102djTiMA7Yuh13IARK2g8c99JtoB8CFG9J2jBmHAW9tDJBth-fVVCCbpggev4T8iNtuGiMkB0PG_hXSOTppbZfgYt_P0N9fP__c3Vfrh9-rux_rynHNc8VJy4QgtfPagvSybYR0ras9ZVzyjVeCek9tEQ-N4JbXgtcUwG0Yd8wqws_Q9cfdbRyfJ0jZ9CHNAov8cUpGESaFqFkBr_bgtOnBm20MvY2vZv-aMv-yn9vkbNcW5y6kA0a1VErx-c7tBwfF1S5ANMkFGBz4EMFl48dgKDFzhGaOxMyRGE3Me4Rmxd8AowGLnQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80276642</pqid></control><display><type>article</type><title>[4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes</title><source>MEDLINE</source><source>ScienceDirect eBooks</source><source>Access via ScienceDirect (Elsevier)</source><creator>Negulescu, Paul A. ; Machen, Terry E.</creator><creatorcontrib>Negulescu, Paul A. ; Machen, Terry E.</creatorcontrib><description>This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits. General methods for loading the dyes into cells, attaching the glands to coverslips and perfusing them with different solutions using flow-through chambers will be described. Techniques for assessing intracellular dye behavior and calibrating the dye fluorescence in cells will be discussed. In addition, approaches for identifying specific membrane transport systems and for measuring rates of transport by these mechanisms will be presented. One advantage of using fluorescent probes is that the same rationing system can be used for measuring any of the dyes discussed in this chapter. This is particularly advantageous because there are many potential interactions among the various ions and ion transporters. With such considerations as guidelines, fluorescent ion indicators should continue to provide unique insights into cellular physiology for many years.</description><identifier>ISSN: 0076-6879</identifier><identifier>ISBN: 0121820939</identifier><identifier>ISBN: 9780121820930</identifier><identifier>EISSN: 1557-7988</identifier><identifier>DOI: 10.1016/0076-6879(90)92062-I</identifier><identifier>PMID: 2074799</identifier><identifier>CODEN: MENZAU</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Science & Technology</publisher><subject>Animals ; Biological and medical sciences ; Biological Transport ; Calcium - metabolism ; Cell Membrane - metabolism ; Cell physiology ; Electrolytes - metabolism ; Epithelium - metabolism ; Fluorescent Dyes ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Ions ; Membrane and intracellular transports ; Molecular and cellular biology ; Mucous Membrane - cytology ; Mucous Membrane - metabolism ; Parietal Cells, Gastric - cytology ; Parietal Cells, Gastric - metabolism ; Rabbits ; Sodium - metabolism ; Spectrometry, Fluorescence - methods ; Urodela</subject><ispartof>Methods in Enzymology, 1990, Vol.192, p.38-81</ispartof><rights>1990</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-30f26604cd9ae7d7f567cfc4d12373bd861dd1a479e563a346341eecb23c2a803</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0076-6879(90)92062-I$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,779,780,784,793,3459,3550,4024,11288,27923,27924,27925,45810,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19788832$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2074799$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Negulescu, Paul A.</creatorcontrib><creatorcontrib>Machen, Terry E.</creatorcontrib><title>[4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes</title><title>Methods in Enzymology</title><addtitle>Methods Enzymol</addtitle><description>This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits. General methods for loading the dyes into cells, attaching the glands to coverslips and perfusing them with different solutions using flow-through chambers will be described. Techniques for assessing intracellular dye behavior and calibrating the dye fluorescence in cells will be discussed. In addition, approaches for identifying specific membrane transport systems and for measuring rates of transport by these mechanisms will be presented. One advantage of using fluorescent probes is that the same rationing system can be used for measuring any of the dyes discussed in this chapter. This is particularly advantageous because there are many potential interactions among the various ions and ion transporters. With such considerations as guidelines, fluorescent ion indicators should continue to provide unique insights into cellular physiology for many years.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Calcium - metabolism</subject><subject>Cell Membrane - metabolism</subject><subject>Cell physiology</subject><subject>Electrolytes - metabolism</subject><subject>Epithelium - metabolism</subject><subject>Fluorescent Dyes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Ions</subject><subject>Membrane and intracellular transports</subject><subject>Molecular and cellular biology</subject><subject>Mucous Membrane - cytology</subject><subject>Mucous Membrane - metabolism</subject><subject>Parietal Cells, Gastric - cytology</subject><subject>Parietal Cells, Gastric - metabolism</subject><subject>Rabbits</subject><subject>Sodium - metabolism</subject><subject>Spectrometry, Fluorescence - methods</subject><subject>Urodela</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0121820939</isbn><isbn>9780121820930</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kUlrHDEQhYVjMxkv_8AGXWLiQydaurVcDMEk8cCAL8kpBKGRqrFML2NJPcb_3mp7mLrUob4q3quH0CUl3yih4jshUlRCSf1VkxvNiGDV6ggtadPISmqlPqFTQhlVjGiuj9HywH9GFyk9kVKNqFVDF2jBiKyl1kv0-K_-j1dDjtZB102djTiMA7Yuh13IARK2g8c99JtoB8CFG9J2jBmHAW9tDJBth-fVVCCbpggev4T8iNtuGiMkB0PG_hXSOTppbZfgYt_P0N9fP__c3Vfrh9-rux_rynHNc8VJy4QgtfPagvSybYR0ras9ZVzyjVeCek9tEQ-N4JbXgtcUwG0Yd8wqws_Q9cfdbRyfJ0jZ9CHNAov8cUpGESaFqFkBr_bgtOnBm20MvY2vZv-aMv-yn9vkbNcW5y6kA0a1VErx-c7tBwfF1S5ANMkFGBz4EMFl48dgKDFzhGaOxMyRGE3Me4Rmxd8AowGLnQ</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Negulescu, Paul A.</creator><creator>Machen, Terry E.</creator><general>Elsevier Science & Technology</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>[4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes</title><author>Negulescu, Paul A. ; Machen, Terry E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-30f26604cd9ae7d7f567cfc4d12373bd861dd1a479e563a346341eecb23c2a803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport</topic><topic>Calcium - metabolism</topic><topic>Cell Membrane - metabolism</topic><topic>Cell physiology</topic><topic>Electrolytes - metabolism</topic><topic>Epithelium - metabolism</topic><topic>Fluorescent Dyes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Ions</topic><topic>Membrane and intracellular transports</topic><topic>Molecular and cellular biology</topic><topic>Mucous Membrane - cytology</topic><topic>Mucous Membrane - metabolism</topic><topic>Parietal Cells, Gastric - cytology</topic><topic>Parietal Cells, Gastric - metabolism</topic><topic>Rabbits</topic><topic>Sodium - metabolism</topic><topic>Spectrometry, Fluorescence - methods</topic><topic>Urodela</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Negulescu, Paul A.</creatorcontrib><creatorcontrib>Machen, Terry E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Methods in Enzymology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Negulescu, Paul A.</au><au>Machen, Terry E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>[4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes</atitle><jtitle>Methods in Enzymology</jtitle><addtitle>Methods Enzymol</addtitle><date>1990</date><risdate>1990</risdate><volume>192</volume><spage>38</spage><epage>81</epage><pages>38-81</pages><issn>0076-6879</issn><eissn>1557-7988</eissn><isbn>0121820939</isbn><isbn>9780121820930</isbn><coden>MENZAU</coden><abstract>This chapter discusses the methods used for measuring intracellular pH, [Cai2+] (Cai2+, with Fura-2) and [Na +] in single, identified cells of gastric glands isolated from rabbits. General methods for loading the dyes into cells, attaching the glands to coverslips and perfusing them with different solutions using flow-through chambers will be described. Techniques for assessing intracellular dye behavior and calibrating the dye fluorescence in cells will be discussed. In addition, approaches for identifying specific membrane transport systems and for measuring rates of transport by these mechanisms will be presented. One advantage of using fluorescent probes is that the same rationing system can be used for measuring any of the dyes discussed in this chapter. This is particularly advantageous because there are many potential interactions among the various ions and ion transporters. With such considerations as guidelines, fluorescent ion indicators should continue to provide unique insights into cellular physiology for many years.</abstract><cop>San Diego, CA</cop><pub>Elsevier Science & Technology</pub><pmid>2074799</pmid><doi>10.1016/0076-6879(90)92062-I</doi><tpages>44</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0076-6879 |
| ispartof | Methods in Enzymology, 1990, Vol.192, p.38-81 |
| issn | 0076-6879 1557-7988 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80276642 |
| source | MEDLINE; ScienceDirect eBooks; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Biological Transport Calcium - metabolism Cell Membrane - metabolism Cell physiology Electrolytes - metabolism Epithelium - metabolism Fluorescent Dyes Fundamental and applied biological sciences. Psychology In Vitro Techniques Ions Membrane and intracellular transports Molecular and cellular biology Mucous Membrane - cytology Mucous Membrane - metabolism Parietal Cells, Gastric - cytology Parietal Cells, Gastric - metabolism Rabbits Sodium - metabolism Spectrometry, Fluorescence - methods Urodela |
| title | [4] Intracellular ion activities and membrane transport in parietal cells measured with fluorescent dyes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T04%3A09%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=%5B4%5D%20Intracellular%20ion%20activities%20and%20membrane%20transport%20in%20parietal%20cells%20measured%20with%20fluorescent%20dyes&rft.jtitle=Methods%20in%20Enzymology&rft.au=Negulescu,%20Paul%20A.&rft.date=1990&rft.volume=192&rft.spage=38&rft.epage=81&rft.pages=38-81&rft.issn=0076-6879&rft.eissn=1557-7988&rft.isbn=0121820939&rft.isbn_list=9780121820930&rft.coden=MENZAU&rft_id=info:doi/10.1016/0076-6879(90)92062-I&rft_dat=%3Cproquest_pubme%3E80276642%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80276642&rft_id=info:pmid/2074799&rft_els_id=007668799092062I&rfr_iscdi=true |