[42] Cation transport probes: The amiloride series
This chapter focuses on the cation transport probes that is it describes the various the amiloride series. Several recent studies have used radiolabeled amiloride analogs to identify and characterize binding sites of amiloride analogs in plasma membranes or microsomal membranes derived from cells kn...
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| Veröffentlicht in: | Methods in Enzymology 1990, Vol.191, p.739-755 |
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| container_title | Methods in Enzymology |
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| creator | Kleyman, Thomas R. Cragoe, Edward J. |
| description | This chapter focuses on the cation transport probes that is it describes the various the amiloride series. Several recent studies have used radiolabeled amiloride analogs to identify and characterize binding sites of amiloride analogs in plasma membranes or microsomal membranes derived from cells known to have amilofide-sensitive transporters. Methods for assaying the binding of amiloride analogs to membranes and to detergent-solubllized proteins are outlined in this chapter. The use of amiloride and its analogs in the study of ion transport requires a knowledge of the pharmacology of inhibition of transport proteins, and of effects on enzymes, receptors, and other cellular processes, such as DNA, RNA, and protein synthesis, and cellular metabolism. This chapter reviews the pharmacology of inhibition of these processes by amiloride an its analogs, as well as the use of amiloride analogs as potential probes for the characterization of ion transport systems. |
| doi_str_mv | 10.1016/0076-6879(90)91045-8 |
| format | Article |
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Several recent studies have used radiolabeled amiloride analogs to identify and characterize binding sites of amiloride analogs in plasma membranes or microsomal membranes derived from cells known to have amilofide-sensitive transporters. Methods for assaying the binding of amiloride analogs to membranes and to detergent-solubllized proteins are outlined in this chapter. The use of amiloride and its analogs in the study of ion transport requires a knowledge of the pharmacology of inhibition of transport proteins, and of effects on enzymes, receptors, and other cellular processes, such as DNA, RNA, and protein synthesis, and cellular metabolism. 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Psychology</subject><subject>Membrane and intracellular transports</subject><subject>Methods</subject><subject>Molecular and cellular biology</subject><subject>Sodium - metabolism</subject><subject>Sodium Channels - drug effects</subject><subject>Sodium Channels - physiology</subject><subject>Sodium-Calcium Exchanger</subject><subject>Sodium-Hydrogen Exchangers</subject><subject>Sodium-Potassium-Exchanging ATPase - metabolism</subject><subject>Structure-Activity Relationship</subject><issn>0076-6879</issn><issn>1557-7988</issn><isbn>0121820920</isbn><isbn>9780121820923</isbn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc1LxDAQxYMf6PrxHyj0IKKH6qRtkokHQRa_YMGLnkRCNplipLtdk67gf2_rLjuXObwfw7z3GDvhcMWBy2sAJXOJSl9ouNQcKpHjFhtxIVSuNOI2OwBecCxAF7DDRht-nx2n9AX9CFmhgD22x7UspdAjVrxXxUc2tl1o51kX7Twt2thli9hOKd1kr5-U2Vlo2hg8ZYlioHTEdmvbJDpe70P29nD_On7KJy-Pz-O7Se4qUF1el5aosFZz74QlFHbqATzW3kvwWsrKoag5gvCKez0VDtFXTqBUiLby5SE7X93tn_leUurMLCRHTWPn1C6TQShUKYH34OkaXE5n5M0ihpmNv2ZtstfP1rpNzjZ179KFtMEESK1wwG5XGPWmfgJFk1yguSMfIrnO-DYYDmbowgzZmiFbo8H8d2Gw_AN-g3a9</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Kleyman, Thomas R.</creator><creator>Cragoe, Edward J.</creator><general>Elsevier Science & Technology</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>[42] Cation transport probes: The amiloride series</title><author>Kleyman, Thomas R. ; Cragoe, Edward J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-f3aee2aa91dc5ae85abd00d8fdd60d9664c85f1805d71d9b5c88d4c586788a4d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Affinity Labels</topic><topic>Amiloride - analogs & derivatives</topic><topic>Amiloride - pharmacology</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Calcium - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Cations</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - physiology</topic><topic>Cell physiology</topic><topic>Epithelium - drug effects</topic><topic>Epithelium - physiology</topic><topic>Fundamental and applied biological sciences. 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| ispartof | Methods in Enzymology, 1990, Vol.191, p.739-755 |
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| subjects | Affinity Labels Amiloride - analogs & derivatives Amiloride - pharmacology Animals Biological and medical sciences Biological Transport - drug effects Calcium - metabolism Carrier Proteins - metabolism Cations Cell Membrane - drug effects Cell Membrane - physiology Cell physiology Epithelium - drug effects Epithelium - physiology Fundamental and applied biological sciences. Psychology Membrane and intracellular transports Methods Molecular and cellular biology Sodium - metabolism Sodium Channels - drug effects Sodium Channels - physiology Sodium-Calcium Exchanger Sodium-Hydrogen Exchangers Sodium-Potassium-Exchanging ATPase - metabolism Structure-Activity Relationship |
| title | [42] Cation transport probes: The amiloride series |
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