The accumulation of oxidized isoforms of chicken triosephosphate isomerase during aging and development

Triosephosphate isomerase (TPI) from mammals undergoes two specific deamidations (Asn-15 and Asn-71) which destabilize the isologous dimer and lead to the degradation of the protein. In aging cells and tissues, the deamidated isoforms accumulate apparently due to age-related changes in protein turno...

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Veröffentlicht in:Mechanisms of ageing and development 1990-11, Vol.56 (2), p.179-186
Hauptverfasser: Noelle Gracy, K., Tang, C.Y., Ümit Yüksel, K., Gracy, Robert W.
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container_end_page 186
container_issue 2
container_start_page 179
container_title Mechanisms of ageing and development
container_volume 56
creator Noelle Gracy, K.
Tang, C.Y.
Ümit Yüksel, K.
Gracy, Robert W.
description Triosephosphate isomerase (TPI) from mammals undergoes two specific deamidations (Asn-15 and Asn-71) which destabilize the isologous dimer and lead to the degradation of the protein. In aging cells and tissues, the deamidated isoforms accumulate apparently due to age-related changes in protein turnover. Chicken TPI lacks one of these sites ( i.e., Asn 71 → Lys), but also exhibits unstable isoforms. These isoforms are the result of the specific oxidations which occur both in vitro and in vivo. Electrophoretic analyses of various tissues from chicken show that the most oxidized isoform, which is present in adult tissues, is only present in small quantities in tissues of the newborn chick. Moreover, embryonic tissues contain almost exclusively the fully reduced form of TPI. Thus, it appears that oxidation rather than deamidation constitutes the first step in the degradation of avian TPI. TPI may be the first example of a protein which has evolved two different types of modifications (deamidation and oxidation) which trigger its degradation. The accumulation of both deamidated and oxidized isoforms in different species may provide clues to the underlying basis for the accumulation of modified proteins in aging.
doi_str_mv 10.1016/0047-6374(90)90008-4
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subjects Aging
Aging - metabolism
Amino Acid Sequence
Animals
Chick Embryo
Chicken
Chickens
Development
Isoenzymes - metabolism
Isozymes
Oxidation
Oxidation-Reduction
Tissue Distribution
Triose-Phosphate Isomerase - chemistry
Triose-Phosphate Isomerase - metabolism
Triosephosphate isomerase
title The accumulation of oxidized isoforms of chicken triosephosphate isomerase during aging and development
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