Immunocytochemical localization of the epidermal growth factor receptor in mouse testis
The distribution of the epidermal growth factor receptor (EGFR) in mouse testis was ascertained by immunocytochemical methodology using a polyclonal antibody (RK2) shown previously to recognize the cytoplasmic domain of the human (A431 cells), murine (Swiss 3T3 cells), and chicken (CK 109 cells) EGF...
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| Veröffentlicht in: | Biology of reproduction 1990-12, Vol.43 (6), p.1087-1097 |
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| container_title | Biology of reproduction |
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| creator | Suárez-Quian, C A Niklinski, W |
| description | The distribution of the epidermal growth factor receptor (EGFR) in mouse testis was ascertained by immunocytochemical methodology
using a polyclonal antibody (RK2) shown previously to recognize the cytoplasmic domain of the human (A431 cells), murine (Swiss
3T3 cells), and chicken (CK 109 cells) EGFR. Initial studies performed to determine the usefulness of this antibody as a probe
of the murine EGFR in testis employed two murine cell lines, TM4 and MA10, of Sertoli cell and Leydig cell origin, respectively,
in which a physiological response of EGF and specific binding of iodinated EGF has been demonstrated. Western blotting in
membrane preparations of TM4 and MA10 revealed only one prominent band at 170 kDa. Immunocytochemical localization in TM4
and MA10 cells illustrated a plasma membrane distribution of the receptor. Western blotting of membrane fractions prepared
from testis also revealed a specific band at 170 kDa. In the intact testis, the EGFR was immunolocalized specifically in Leydig
cells and Sertoli cells only. These results suggest that the involvement of EGF action in spermatogenesis may occur at the
level of the somatic components of the testes, principally in the Leydig and Sertoli cells. |
| doi_str_mv | 10.1095/biolreprod43.6.1087 |
| format | Article |
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using a polyclonal antibody (RK2) shown previously to recognize the cytoplasmic domain of the human (A431 cells), murine (Swiss
3T3 cells), and chicken (CK 109 cells) EGFR. Initial studies performed to determine the usefulness of this antibody as a probe
of the murine EGFR in testis employed two murine cell lines, TM4 and MA10, of Sertoli cell and Leydig cell origin, respectively,
in which a physiological response of EGF and specific binding of iodinated EGF has been demonstrated. Western blotting in
membrane preparations of TM4 and MA10 revealed only one prominent band at 170 kDa. Immunocytochemical localization in TM4
and MA10 cells illustrated a plasma membrane distribution of the receptor. Western blotting of membrane fractions prepared
from testis also revealed a specific band at 170 kDa. In the intact testis, the EGFR was immunolocalized specifically in Leydig
cells and Sertoli cells only. These results suggest that the involvement of EGF action in spermatogenesis may occur at the
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using a polyclonal antibody (RK2) shown previously to recognize the cytoplasmic domain of the human (A431 cells), murine (Swiss
3T3 cells), and chicken (CK 109 cells) EGFR. Initial studies performed to determine the usefulness of this antibody as a probe
of the murine EGFR in testis employed two murine cell lines, TM4 and MA10, of Sertoli cell and Leydig cell origin, respectively,
in which a physiological response of EGF and specific binding of iodinated EGF has been demonstrated. Western blotting in
membrane preparations of TM4 and MA10 revealed only one prominent band at 170 kDa. Immunocytochemical localization in TM4
and MA10 cells illustrated a plasma membrane distribution of the receptor. Western blotting of membrane fractions prepared
from testis also revealed a specific band at 170 kDa. In the intact testis, the EGFR was immunolocalized specifically in Leydig
cells and Sertoli cells only. These results suggest that the involvement of EGF action in spermatogenesis may occur at the
level of the somatic components of the testes, principally in the Leydig and Sertoli cells.</description><subject>Animals</subject><subject>Cell Line</subject><subject>ErbB Receptors - metabolism</subject><subject>Immunoblotting</subject><subject>Immunohistochemistry</subject><subject>Leydig Cells - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Sertoli Cells - metabolism</subject><subject>testes</subject><subject>Testis - metabolism</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkF1LwzAUhoMoc05_gQi98q4zH22aXMrwYzDwRvEyZOnpGmmbmbQU_fVmbDjvvMkJOc95T3gQuiZ4TrDM79bWNR623pUZm_P4JooTNCU5lWlBuThFU4wxTxnj7BxdhPCBMckYZRM0oVQSSYspel-27dA589U7U0NrjW6SxsXTfuveui5xVdLXkMDWluDb2N14N_Z1UmnTO594MLDdXWyXtG4IkPQQehsu0VmlmwBXhzpDb48Pr4vndPXytFzcr1KTFaxPARNGsSTE5FBiI6CoBNNlrjMhuayo4AyKrGRlzgnjRNBcFDhjUGmerTkWbIZu97lRw-cQV6vWBgNNozuI31ECRxOZLP4FSUyOqIwg24PGuxA8VGrrbav9lyJY7byrv94VVzvvcermED-sWyh_Zw6ij-tru6lH60GFKLOJNFPjOB6DfgBe3I8w</recordid><startdate>19901201</startdate><enddate>19901201</enddate><creator>Suárez-Quian, C A</creator><creator>Niklinski, W</creator><general>Society for the Study of Reproduction</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TO</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19901201</creationdate><title>Immunocytochemical localization of the epidermal growth factor receptor in mouse testis</title><author>Suárez-Quian, C A ; Niklinski, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-e01320911c5ed0c8e7f83ad5a48969f2863e74d3d56136182587043efa64b6083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Animals</topic><topic>Cell Line</topic><topic>ErbB Receptors - metabolism</topic><topic>Immunoblotting</topic><topic>Immunohistochemistry</topic><topic>Leydig Cells - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Sertoli Cells - metabolism</topic><topic>testes</topic><topic>Testis - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suárez-Quian, C A</creatorcontrib><creatorcontrib>Niklinski, W</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suárez-Quian, C A</au><au>Niklinski, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunocytochemical localization of the epidermal growth factor receptor in mouse testis</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>1990-12-01</date><risdate>1990</risdate><volume>43</volume><issue>6</issue><spage>1087</spage><epage>1097</epage><pages>1087-1097</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><abstract>The distribution of the epidermal growth factor receptor (EGFR) in mouse testis was ascertained by immunocytochemical methodology
using a polyclonal antibody (RK2) shown previously to recognize the cytoplasmic domain of the human (A431 cells), murine (Swiss
3T3 cells), and chicken (CK 109 cells) EGFR. Initial studies performed to determine the usefulness of this antibody as a probe
of the murine EGFR in testis employed two murine cell lines, TM4 and MA10, of Sertoli cell and Leydig cell origin, respectively,
in which a physiological response of EGF and specific binding of iodinated EGF has been demonstrated. Western blotting in
membrane preparations of TM4 and MA10 revealed only one prominent band at 170 kDa. Immunocytochemical localization in TM4
and MA10 cells illustrated a plasma membrane distribution of the receptor. Western blotting of membrane fractions prepared
from testis also revealed a specific band at 170 kDa. In the intact testis, the EGFR was immunolocalized specifically in Leydig
cells and Sertoli cells only. These results suggest that the involvement of EGF action in spermatogenesis may occur at the
level of the somatic components of the testes, principally in the Leydig and Sertoli cells.</abstract><cop>United States</cop><pub>Society for the Study of Reproduction</pub><pmid>2291927</pmid><doi>10.1095/biolreprod43.6.1087</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0006-3363 |
| ispartof | Biology of reproduction, 1990-12, Vol.43 (6), p.1087-1097 |
| issn | 0006-3363 1529-7268 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80268497 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Cell Line ErbB Receptors - metabolism Immunoblotting Immunohistochemistry Leydig Cells - metabolism Male Mice Sertoli Cells - metabolism testes Testis - metabolism |
| title | Immunocytochemical localization of the epidermal growth factor receptor in mouse testis |
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