Identification and characterization of de novo-synthesized porcine oviductal secretory proteins
Oviductal secretory products provide a biochemical environment important for establishment of pregnancy. A previous study identified three de novo-synthesized glycoproteins by one-dimensional SDS-PAGE as well as increased incorporation of [3H]Leu into secretory protein by whole oviduct and ampulla a...
Gespeichert in:
Veröffentlicht in: | Biology of reproduction 1990-12, Vol.43 (6), p.929-938 |
---|---|
Hauptverfasser: | , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Oviductal secretory products provide a biochemical environment important for establishment of pregnancy. A previous study
identified three de novo-synthesized glycoproteins by one-dimensional SDS-PAGE as well as increased incorporation of [3H]Leu
into secretory protein by whole oviduct and ampulla associated with proestrus, estrus, and metestrus only. Here, our objective
was to further identify and characterize oviductal secretory proteins, specifically 115,000- and 85,000-Mr estrus-associated
proteins (EAP). Two-dimensional SDS-PAGE resolved the 115,000-Mr protein into two proteins of 100,000 Mr, one basic and one
acidic, and the 85,000-Mr protein into 75,000- and 85,000-Mr species (pI less than 4.0). Differential secretion of proteins
between ampulla and isthmus was indicated. The 100,000-, 75,000-, and 85,000-Mr proteins were synthesized by ampulla during
estrus but not by isthmus nor by uterine endometrium. De novo-synthesized EAP were labeled with glucosamine, Leu, and Met,
and the 75,000-85,000-Mr proteins from ampulla and a 30,000-Mr family from isthmus were labeled with fucose. Inorganic [35S]sulfate
labeled three EAP. Fractionation of culture medium by gel filtration demonstrated differences between products secreted by
ampulla and isthmus and suggested that some EAP may be found as high-molecular weight forms in the native state. Results indicate
that porcine oviductal tissue synthesizes specific EAP at the time of fertilization and early cleavage-stage embryonic development,
that there are differences in the type and distribution of glycoproteins from ampulla and isthmus, and that post-translational
modifications occur with the addition of glucosamine, fucose, and inorganic sulfate. |
---|---|
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod43.6.929 |