Detection of free radicals during the cellular metabolism of adriamycin

Experiments were conducted to determine which free radicals are generated during the metabolism of adriamycin (ADM) by canine tracheal epithelial (CTE) cells, guinea pig enterocytes, and rat hepatocytes. The technique employed in this study was spin trapping; the spin trap utilized was 5,5-dimethyl-...

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Veröffentlicht in:Free radical biology & medicine 1990, Vol.9 (5), p.415-421
Hauptverfasser: Turner, Marvin J., Everman, David B., Ellington, Sharon P., Fields, Charles E.
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creator Turner, Marvin J.
Everman, David B.
Ellington, Sharon P.
Fields, Charles E.
description Experiments were conducted to determine which free radicals are generated during the metabolism of adriamycin (ADM) by canine tracheal epithelial (CTE) cells, guinea pig enterocytes, and rat hepatocytes. The technique employed in this study was spin trapping; the spin trap utilized was 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). The spin adduct 2-hydroxy-5,5-dimethyl-1-pyrrolidinyloxyl (DMPO-OH) was observed during the metabolism of ADM by CTE cells. However, the addition of dimethyl sulfoxide to the in vitro system suggested that superoxide is initially spin trapped by nitrone, and that the adduct 2-hydroperoxy-5, 5-dimethyl-1-pyrrolidinyloxyl (DMPO-OOH) is rapidly bioreduced to afford DMPO-OH. The addition of superoxide dismutase to the system indicated that superoxide generation was primarily intracellular. The adriamycin semiquinone free radical (ADM-SQ) was produced during the metabolism by enterocytes and hepatocytes. The rate of the production of ADM-SQ was enhanced under anaerobic conditions, suggesting that molecular oxygen was responsible for the degradation of this carbon-centered free radical. However, spin trapping of oxygen radicals was not observed; this observation suggests that these reactive intermediates are not produced at concentrations sufficient for detection by spin-trapping experiments.
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subjects Adriamycin
Adriamycin semiquinone free radical
Animals
Antineoplastic agents
Biological and medical sciences
Chemotherapy
Dogs
Doxorubicin - chemistry
Doxorubicin - metabolism
Drug metabolism
Electron Spin Resonance Spectroscopy
Free Radicals
Guinea Pigs
Hydroxides - metabolism
Hydroxyl Radical
In Vitro Techniques
Intestinal Mucosa - metabolism
Liver - metabolism
Male
Medical sciences
Pharmacology. Drug treatments
Rats
Rats, Inbred F344
Spin trapping
Superoxide
Superoxides - metabolism
Trachea - metabolism
title Detection of free radicals during the cellular metabolism of adriamycin
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