DNA-mediated transformation system for Dictyostelium discoideum
We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomousl...
Gespeichert in:
| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1982-12, Vol.79 (23), p.7356-7360 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 7360 |
|---|---|
| container_issue | 23 |
| container_start_page | 7356 |
| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 79 |
| creator | Hirth, K.P Edwards, C.A Firtel, R.A |
| description | We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10-6and 10-4of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA. |
| doi_str_mv | 10.1073/pnas.79.23.7356 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_80264562</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>13086</jstor_id><sourcerecordid>13086</sourcerecordid><originalsourceid>FETCH-LOGICAL-c551t-13eb3aa4b0638b92e18183dab896be1d6e90d1875096dc303e6f982c796420e13</originalsourceid><addsrcrecordid>eNp9kM1v1DAQxS0EKtvCGQkJyAlO2Y7txB8HhKqWFqQKDtCz5SST4iqJF9tB7H9fR7st9NKTZb_fm3l-hLyisKYg-fFmsnEt9ZrxteS1eEJWFDQtRaXhKVkBMFmqilXPyWGMNwCgawUH5EAwLRSHFfl09u2kHLFzNmFXpGCn2Psw2uT8VMRtTDgW-aE4c23a-nwd3DwWnYutdx3O4wvyrLdDxJf784hcnX_-efqlvPx-8fX05LJs65qmknJsuLVVA4KrRjOkiire2UZp0SDtBGroqJI1aNG1HDiKXivWSi0qBkj5Efm4m7uZmxy3xSlnHcwmuNGGrfHWmYfK5H6Za__H8EpyrrL__d4f_O8ZYzJj_gMOg53Qz9EoYKKqBcvg8Q5sg48xYH-_g4JZKjdL5UZqw7hZKs-ON_9Hu-f3HWf97V5fjHfqgwEfHgVMPw9Dwr8pk6935E1MPvxLxkEtY97txN56Y6-Di-bqB4MsslpKoRm_BXeTqFA</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>80264562</pqid></control><display><type>article</type><title>DNA-mediated transformation system for Dictyostelium discoideum</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Hirth, K.P ; Edwards, C.A ; Firtel, R.A</creator><creatorcontrib>Hirth, K.P ; Edwards, C.A ; Firtel, R.A</creatorcontrib><description>We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10-6and 10-4of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.79.23.7356</identifier><identifier>PMID: 6296830</identifier><language>eng</language><publisher>United States: National Academy of Sciences of the United States of America</publisher><subject>Actins ; Aminoglycosides - pharmacology ; Bacteria ; Cell growth ; Cell lines ; Dictyostelium - genetics ; DNA ; DNA - genetics ; DNA Transposable Elements ; Drug Resistance ; fungi ; Genes ; Genetic Vectors ; Neomycin - pharmacology ; Plasmids ; Transformation, Genetic ; Transformed cell line ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1982-12, Vol.79 (23), p.7356-7360</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-13eb3aa4b0638b92e18183dab896be1d6e90d1875096dc303e6f982c796420e13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/79/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/13086$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/13086$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6296830$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hirth, K.P</creatorcontrib><creatorcontrib>Edwards, C.A</creatorcontrib><creatorcontrib>Firtel, R.A</creatorcontrib><title>DNA-mediated transformation system for Dictyostelium discoideum</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10-6and 10-4of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.</description><subject>Actins</subject><subject>Aminoglycosides - pharmacology</subject><subject>Bacteria</subject><subject>Cell growth</subject><subject>Cell lines</subject><subject>Dictyostelium - genetics</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA Transposable Elements</subject><subject>Drug Resistance</subject><subject>fungi</subject><subject>Genes</subject><subject>Genetic Vectors</subject><subject>Neomycin - pharmacology</subject><subject>Plasmids</subject><subject>Transformation, Genetic</subject><subject>Transformed cell line</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1v1DAQxS0EKtvCGQkJyAlO2Y7txB8HhKqWFqQKDtCz5SST4iqJF9tB7H9fR7st9NKTZb_fm3l-hLyisKYg-fFmsnEt9ZrxteS1eEJWFDQtRaXhKVkBMFmqilXPyWGMNwCgawUH5EAwLRSHFfl09u2kHLFzNmFXpGCn2Psw2uT8VMRtTDgW-aE4c23a-nwd3DwWnYutdx3O4wvyrLdDxJf784hcnX_-efqlvPx-8fX05LJs65qmknJsuLVVA4KrRjOkiire2UZp0SDtBGroqJI1aNG1HDiKXivWSi0qBkj5Efm4m7uZmxy3xSlnHcwmuNGGrfHWmYfK5H6Za__H8EpyrrL__d4f_O8ZYzJj_gMOg53Qz9EoYKKqBcvg8Q5sg48xYH-_g4JZKjdL5UZqw7hZKs-ON_9Hu-f3HWf97V5fjHfqgwEfHgVMPw9Dwr8pk6935E1MPvxLxkEtY97txN56Y6-Di-bqB4MsslpKoRm_BXeTqFA</recordid><startdate>19821201</startdate><enddate>19821201</enddate><creator>Hirth, K.P</creator><creator>Edwards, C.A</creator><creator>Firtel, R.A</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19821201</creationdate><title>DNA-mediated transformation system for Dictyostelium discoideum</title><author>Hirth, K.P ; Edwards, C.A ; Firtel, R.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-13eb3aa4b0638b92e18183dab896be1d6e90d1875096dc303e6f982c796420e13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Actins</topic><topic>Aminoglycosides - pharmacology</topic><topic>Bacteria</topic><topic>Cell growth</topic><topic>Cell lines</topic><topic>Dictyostelium - genetics</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA Transposable Elements</topic><topic>Drug Resistance</topic><topic>fungi</topic><topic>Genes</topic><topic>Genetic Vectors</topic><topic>Neomycin - pharmacology</topic><topic>Plasmids</topic><topic>Transformation, Genetic</topic><topic>Transformed cell line</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hirth, K.P</creatorcontrib><creatorcontrib>Edwards, C.A</creatorcontrib><creatorcontrib>Firtel, R.A</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hirth, K.P</au><au>Edwards, C.A</au><au>Firtel, R.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA-mediated transformation system for Dictyostelium discoideum</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1982-12-01</date><risdate>1982</risdate><volume>79</volume><issue>23</issue><spage>7356</spage><epage>7360</epage><pages>7356-7360</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><abstract>We have established a transformation system for Dictyostelium discoideum. The transformation vector contains the protein coding region of the Tn5 neomycin resistance gene fused to the proposed promoter of the Dictyostelium actin 8 gene; the vector also contains a sequence that acts as an autonomously replicating sequence (ars) in yeast. Using this vector, we can transform Dictyostelium vegetative amoebae to be resistant to aminoglycoside G418 at a frequency of between 10-6and 10-4of the input cells. The transformed cell lines are stable and contain vector sequences integrated within chromosomal DNA.</abstract><cop>United States</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>6296830</pmid><doi>10.1073/pnas.79.23.7356</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1982-12, Vol.79 (23), p.7356-7360 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80264562 |
| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Actins Aminoglycosides - pharmacology Bacteria Cell growth Cell lines Dictyostelium - genetics DNA DNA - genetics DNA Transposable Elements Drug Resistance fungi Genes Genetic Vectors Neomycin - pharmacology Plasmids Transformation, Genetic Transformed cell line Yeasts |
| title | DNA-mediated transformation system for Dictyostelium discoideum |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-07T09%3A37%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA-mediated%20transformation%20system%20for%20Dictyostelium%20discoideum&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=Hirth,%20K.P&rft.date=1982-12-01&rft.volume=79&rft.issue=23&rft.spage=7356&rft.epage=7360&rft.pages=7356-7360&rft.issn=0027-8424&rft.eissn=1091-6490&rft_id=info:doi/10.1073/pnas.79.23.7356&rft_dat=%3Cjstor_proqu%3E13086%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=80264562&rft_id=info:pmid/6296830&rft_jstor_id=13086&rfr_iscdi=true |