Isolation and separation of the glycan strands from murein of Escherichia coli by reversed-phase high-performance liquid chromatography

The length distribution of the glycan strands in the murein (peptidoglycan) sacculus of Escherichia coli has been analyzed after solubilization of the murein by complete digestion with human serum amidase. The glycan strands released were separated according to length by reversed-phase HPLC on wide-...

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Veröffentlicht in:	Analytical biochemistry 1990-10, Vol.190 (1), p.120-128
Hauptverfasser:	Harz, Hartmann, Burgdorf, Knut, Höltje, Joachim-Volker
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - metabolism Amino Acid Sequence Carbohydrate Sequence Chromatography, High Pressure Liquid - methods Escherichia coli - analysis Humans Hydrogen-Ion Concentration Hydrolysis Molecular Sequence Data Molecular Weight Muramic Acids - metabolism Muramidase - metabolism Peptidoglycan - isolation & purification Peptidoglycan - metabolism Polysaccharides - isolation & purification Polysaccharides - metabolism
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