A family of expression vectors based on the rrnB P2 promoter of Escherichia coli

We describe here the construction of a family of expression vectors, based on the P2 promoter of the Escherichia coli rrnB gene by removing regulatory sequences downstream of the Pribnow-box and replacing them with the lac operator. These vectors allow cloning of foreign genes in such a way that the...

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Veröffentlicht in:	Journal of biotechnology 1990-10, Vol.16 (1-2), p.49-55
Hauptverfasser:	LUKACSOVICH, T, OROSZ, A, BALIKO, G, VENETIANER, P
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence Biological and medical sciences Biotechnology Cloning, Molecular DNA, Bacterial - chemistry Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Molecular Sequence Data Plasmids Promoter Regions, Genetic Protein Biosynthesis Recombinant Fusion Proteins - biosynthesis Recombinant Fusion Proteins - genetics Regulatory Sequences, Nucleic Acid Restriction Mapping RNA, Bacterial - genetics RNA, Ribosomal - genetics Transcription, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons
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