Harvesting recombinant microbial cells using crossflow filtration
A contained, crossflow filtration (CFF) membrane system is described for harvesting Saccharomyces cerevisiae and Escherichia coli cells. This system is portable and can be cleaned and sanitized in place. Low- and high-cell density (LCD, HCD) fermentations of recombinant cells in 10- to 200-l volumes...
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| Veröffentlicht in: | Enzyme and microbial technology 1990-09, Vol.12 (9), p.647-652 |
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| container_title | Enzyme and microbial technology |
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| creator | Bailey, F.James Warf, R.Thomas Maigetter, Robert Z. |
| description | A contained, crossflow filtration (CFF) membrane system is described for harvesting Saccharomyces cerevisiae and
Escherichia coli cells. This system is portable and can be cleaned and sanitized in place. Low- and high-cell density (LCD, HCD) fermentations of recombinant cells in 10- to 200-l volumes were used as the starting material. LCD fermentations, up to 8.3 g l
−1 dry weight (dcw) of S. cerevisiae, with volumes of 10 to 200 l were harvested and diafiltered in 0.5 and 1.5 h, respectively. HCD 200-l fermentations of
S. cerevisiae (47–63 g l
−1 dcw) were harvested and diafiltered in approximately 2 h.
E. coli fermentations, LCD and HCD (up to 16.2 g l
−1 dcw), of 200-l volumes were harvested and diafiltered in 2.3 h while employing 14 and 75 ft
2 of membrane area, respectively. Using hollow fiber or flat sheet membranes from different sources, cell harvesting times were less than 2.5 h. These studies demonstrate that CFF is an efficient method for harvesting and diafiltering recombinant
S. cerevisiae and
E. coli cells from fermentation broth. |
| doi_str_mv | 10.1016/0141-0229(90)90002-8 |
| format | Article |
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Escherichia coli cells. This system is portable and can be cleaned and sanitized in place. Low- and high-cell density (LCD, HCD) fermentations of recombinant cells in 10- to 200-l volumes were used as the starting material. LCD fermentations, up to 8.3 g l
−1 dry weight (dcw) of S. cerevisiae, with volumes of 10 to 200 l were harvested and diafiltered in 0.5 and 1.5 h, respectively. HCD 200-l fermentations of
S. cerevisiae (47–63 g l
−1 dcw) were harvested and diafiltered in approximately 2 h.
E. coli fermentations, LCD and HCD (up to 16.2 g l
−1 dcw), of 200-l volumes were harvested and diafiltered in 2.3 h while employing 14 and 75 ft
2 of membrane area, respectively. Using hollow fiber or flat sheet membranes from different sources, cell harvesting times were less than 2.5 h. These studies demonstrate that CFF is an efficient method for harvesting and diafiltering recombinant
S. cerevisiae and
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Escherichia coli cells. This system is portable and can be cleaned and sanitized in place. Low- and high-cell density (LCD, HCD) fermentations of recombinant cells in 10- to 200-l volumes were used as the starting material. LCD fermentations, up to 8.3 g l
−1 dry weight (dcw) of S. cerevisiae, with volumes of 10 to 200 l were harvested and diafiltered in 0.5 and 1.5 h, respectively. HCD 200-l fermentations of
S. cerevisiae (47–63 g l
−1 dcw) were harvested and diafiltered in approximately 2 h.
E. coli fermentations, LCD and HCD (up to 16.2 g l
−1 dcw), of 200-l volumes were harvested and diafiltered in 2.3 h while employing 14 and 75 ft
2 of membrane area, respectively. Using hollow fiber or flat sheet membranes from different sources, cell harvesting times were less than 2.5 h. These studies demonstrate that CFF is an efficient method for harvesting and diafiltering recombinant
S. cerevisiae and
E. coli cells from fermentation broth.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Cell harvesting</subject><subject>crossflow filtration</subject><subject>E. coli</subject><subject>Escherichia coli - metabolism</subject><subject>Fermentation</subject><subject>filtration</subject><subject>Filtration - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>harvesting</subject><subject>Methods. Procedures. Technologies</subject><subject>Others</subject><subject>recombinant</subject><subject>Saccharomyces</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - metabolism</subject><subject>Various methods and equipments</subject><issn>0141-0229</issn><issn>1879-0909</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctKxTAURYMoen38gUInig6qJ4-2ORNBRL2C4ETHIU1TifRxTVrFvze1F53pKJCzstlnhZBDCucUaH4BVNAUGMNThDMEAJbKDbKgssAUEHCTLH6QHbIbwmtkqBCwTbYpz3MJ-YJcLbV_t2Fw3Uvirenb0nW6G5LWGd-XTjeJsU0TkjFMRLwLoW76j6R2zeD14Ppun2zVugn2YH3ukefbm6frZfrweHd_ffWQGsGzIZWSioLrkmcahdCIrK4LyrE0rMxQ5Mjr3HJZWlbovMq51FCKrLDGGgmMIt8jJ3PuyvdvY6ysWhemcrqz_RhUpARFKf8FaVYwLhAiKGbwey1va7XyrtX-U1FQk2I1-VOTP4WgvhWrKf9onT-Wra1-H81O4_x4PdfB6Kb2ujMu_GKYMVqAiNzlzNlo7d1Zr4JxtjO2cvEnBlX17u8iX2HElss</recordid><startdate>199009</startdate><enddate>199009</enddate><creator>Bailey, F.James</creator><creator>Warf, R.Thomas</creator><creator>Maigetter, Robert Z.</creator><general>Elsevier Inc</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>199009</creationdate><title>Harvesting recombinant microbial cells using crossflow filtration</title><author>Bailey, F.James ; Warf, R.Thomas ; Maigetter, Robert Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c435t-881473ab35a944a992ff7139bc2b594693f6e38be27a6d638a0b457ecec802193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Cell harvesting</topic><topic>crossflow filtration</topic><topic>E. coli</topic><topic>Escherichia coli - metabolism</topic><topic>Fermentation</topic><topic>filtration</topic><topic>Filtration - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>harvesting</topic><topic>Methods. Procedures. Technologies</topic><topic>Others</topic><topic>recombinant</topic><topic>Saccharomyces</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - metabolism</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bailey, F.James</creatorcontrib><creatorcontrib>Warf, R.Thomas</creatorcontrib><creatorcontrib>Maigetter, Robert Z.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Enzyme and microbial technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bailey, F.James</au><au>Warf, R.Thomas</au><au>Maigetter, Robert Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Harvesting recombinant microbial cells using crossflow filtration</atitle><jtitle>Enzyme and microbial technology</jtitle><addtitle>Enzyme Microb Technol</addtitle><date>1990-09</date><risdate>1990</risdate><volume>12</volume><issue>9</issue><spage>647</spage><epage>652</epage><pages>647-652</pages><issn>0141-0229</issn><eissn>1879-0909</eissn><coden>EMTED2</coden><abstract>A contained, crossflow filtration (CFF) membrane system is described for harvesting Saccharomyces cerevisiae and
Escherichia coli cells. This system is portable and can be cleaned and sanitized in place. Low- and high-cell density (LCD, HCD) fermentations of recombinant cells in 10- to 200-l volumes were used as the starting material. LCD fermentations, up to 8.3 g l
−1 dry weight (dcw) of S. cerevisiae, with volumes of 10 to 200 l were harvested and diafiltered in 0.5 and 1.5 h, respectively. HCD 200-l fermentations of
S. cerevisiae (47–63 g l
−1 dcw) were harvested and diafiltered in approximately 2 h.
E. coli fermentations, LCD and HCD (up to 16.2 g l
−1 dcw), of 200-l volumes were harvested and diafiltered in 2.3 h while employing 14 and 75 ft
2 of membrane area, respectively. Using hollow fiber or flat sheet membranes from different sources, cell harvesting times were less than 2.5 h. These studies demonstrate that CFF is an efficient method for harvesting and diafiltering recombinant
S. cerevisiae and
E. coli cells from fermentation broth.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>1366806</pmid><doi>10.1016/0141-0229(90)90002-8</doi><tpages>6</tpages></addata></record> |
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| identifier | ISSN: 0141-0229 |
| ispartof | Enzyme and microbial technology, 1990-09, Vol.12 (9), p.647-652 |
| issn | 0141-0229 1879-0909 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80241988 |
| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Biotechnology Biotechnology - methods Cell harvesting crossflow filtration E. coli Escherichia coli - metabolism Fermentation filtration Filtration - methods Fundamental and applied biological sciences. Psychology harvesting Methods. Procedures. Technologies Others recombinant Saccharomyces Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Various methods and equipments |
| title | Harvesting recombinant microbial cells using crossflow filtration |
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