Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation
An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) we...
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| Veröffentlicht in: | MICROBIOLOGY and IMMUNOLOGY 1990, Vol.34(8), pp.703-708 |
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| container_title | MICROBIOLOGY and IMMUNOLOGY |
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| creator | Hamashima, Hajime Nakano, Toshikazu Tamura, Shinobu Arai, Taketoshi |
| description | An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains. |
| doi_str_mv | 10.1111/j.1348-0421.1990.tb01047.x |
| format | Article |
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Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains.</description><identifier>ISSN: 0385-5600</identifier><identifier>EISSN: 1348-0421</identifier><identifier>DOI: 10.1111/j.1348-0421.1990.tb01047.x</identifier><identifier>PMID: 2280728</identifier><identifier>CODEN: MIIMDV</identifier><language>eng</language><publisher>Tokyo: Blackwell Publishing Ltd</publisher><subject>Bacteriology ; Biological and medical sciences ; Biotechnology ; DNA ; DNA, Bacterial - genetics ; DNA, Recombinant ; Electrochemistry ; electroporation ; Fundamental and applied biological sciences. Psychology ; Genetic Vectors ; Microbiology ; Miscellaneous ; Plasmids ; transformation ; Transformation, Bacterial ; Vibrio - genetics ; Vibrio alginolyticus ; Vibrio cholerae ; Vibrio cholerae - genetics ; Vibrio parahaemolyticus ; Vibrio parahaemolyticus - genetics</subject><ispartof>MICROBIOLOGY and IMMUNOLOGY, 1990, Vol.34(8), pp.703-708</ispartof><rights>Center for Academic Publications Japan</rights><rights>owned by Center for Academic Publications Japan (Publisher)</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5097-dd2442ecabf6363d989e1642c49b44c5e6d2ab9f718f9edf23db2085fed395ea3</citedby><cites>FETCH-LOGICAL-c5097-dd2442ecabf6363d989e1642c49b44c5e6d2ab9f718f9edf23db2085fed395ea3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19413445$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2280728$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamashima, Hajime</creatorcontrib><creatorcontrib>Nakano, Toshikazu</creatorcontrib><creatorcontrib>Tamura, Shinobu</creatorcontrib><creatorcontrib>Arai, Taketoshi</creatorcontrib><title>Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation</title><title>MICROBIOLOGY and IMMUNOLOGY</title><addtitle>Microbiology and Immunology</addtitle><description>An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains.</description><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Recombinant</subject><subject>Electrochemistry</subject><subject>electroporation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic Vectors</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Plasmids</subject><subject>transformation</subject><subject>Transformation, Bacterial</subject><subject>Vibrio - genetics</subject><subject>Vibrio alginolyticus</subject><subject>Vibrio cholerae</subject><subject>Vibrio cholerae - genetics</subject><subject>Vibrio parahaemolyticus</subject><subject>Vibrio parahaemolyticus - genetics</subject><issn>0385-5600</issn><issn>1348-0421</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVks1uEzEUhUcIVELhEZBGSCAWneC_-TErqiZNK6VpkAosLY_nTuPgGQd7oiZv0MfG6aQpKxBe2Fc-537X0nEUvcNoiMP6tBxiyooEMYKHmHM07EqEEcuHm2fR4CA9jwaIFmmSZgi9jF55v0SI5KRgR9ERIQUK5SC6n0ALnVbxjZOtr61rZKdtG9s6_q5Lp228kk4uJDTWbINv7U8eBWludft0K9vqUVELa8BJiGeBdJ3g-E53i3hupG90FY9mp3G5jccGVOfsyrqHia-jF7U0Ht7sz-Po2_n45uwimV5PLs9Op4lKEc-TqiKMEVCyrDOa0YoXHHDGiGK8ZEylkFVElrzOcVFzqGpCq5KgIq2hojwFSY-jDz135eyvNfhONNorMEa2YNdeFIgwjDj-pxGnBeMsQ8H48e9GxkmGScpJsH7urcpZ7x3UYuV0I91WYCR20Yql2OUndvmJXbRiH63YhOa3-znrsoHq0LrPMujv97r0Spo6BKq0f5rAWUCzNPi-9L47bWD7Hy8QV5dXD2VAjHvE0nfyFg4M6cJnMCCa8Bk05nkuKBNFv-WIHnS1kE5AGzhJz9G-g80fmJ8iy2meih-ziZifj6Zfp6MLMae_ARea6i0</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Hamashima, Hajime</creator><creator>Nakano, Toshikazu</creator><creator>Tamura, Shinobu</creator><creator>Arai, Taketoshi</creator><general>Blackwell Publishing Ltd</general><general>Center For Academic Publications Japan</general><general>Center for Academic Publications Japan</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation</title><author>Hamashima, Hajime ; Nakano, Toshikazu ; Tamura, Shinobu ; Arai, Taketoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5097-dd2442ecabf6363d989e1642c49b44c5e6d2ab9f718f9edf23db2085fed395ea3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Recombinant</topic><topic>Electrochemistry</topic><topic>electroporation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic Vectors</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Plasmids</topic><topic>transformation</topic><topic>Transformation, Bacterial</topic><topic>Vibrio - genetics</topic><topic>Vibrio alginolyticus</topic><topic>Vibrio cholerae</topic><topic>Vibrio cholerae - genetics</topic><topic>Vibrio parahaemolyticus</topic><topic>Vibrio parahaemolyticus - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamashima, Hajime</creatorcontrib><creatorcontrib>Nakano, Toshikazu</creatorcontrib><creatorcontrib>Tamura, Shinobu</creatorcontrib><creatorcontrib>Arai, Taketoshi</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamashima, Hajime</au><au>Nakano, Toshikazu</au><au>Tamura, Shinobu</au><au>Arai, Taketoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation</atitle><jtitle>MICROBIOLOGY and IMMUNOLOGY</jtitle><addtitle>Microbiology and Immunology</addtitle><date>1990</date><risdate>1990</risdate><volume>34</volume><issue>8</issue><spage>703</spage><epage>708</epage><pages>703-708</pages><issn>0385-5600</issn><eissn>1348-0421</eissn><coden>MIIMDV</coden><abstract>An electroporation procedure for the plasmid-mediated transformation of the genus Vibrio was performed, as part of an effort to develop recombinant DNA techniques for genetic manipulation of the genus Vibrio. Vibrio parahaemolyticus, V. alginolyticus, and V. cholerae non O-1 (9 different strains) were transformed with 3 vector plasmids (pACYC184, pHSG398, and pBR325). The efficiency of transformation was highly dependent on three parameters: the concentration of plasmid DNA; the strength of the electric field; and the combination of plasmid DNA and recipient strain. The drug-resistance genes on the vector plasmid were expressed in the Vibrio strains.</abstract><cop>Tokyo</cop><pub>Blackwell Publishing Ltd</pub><pmid>2280728</pmid><doi>10.1111/j.1348-0421.1990.tb01047.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0385-5600 |
| ispartof | MICROBIOLOGY and IMMUNOLOGY, 1990, Vol.34(8), pp.703-708 |
| issn | 0385-5600 1348-0421 |
| language | eng |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; Open Access Titles of Japan; Alma/SFX Local Collection |
| subjects | Bacteriology Biological and medical sciences Biotechnology DNA DNA, Bacterial - genetics DNA, Recombinant Electrochemistry electroporation Fundamental and applied biological sciences. Psychology Genetic Vectors Microbiology Miscellaneous Plasmids transformation Transformation, Bacterial Vibrio - genetics Vibrio alginolyticus Vibrio cholerae Vibrio cholerae - genetics Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics |
| title | Genetic Transformation of Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio cholerae Non O-1 with Plasmid DNA by Electroporation |
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