Preparative size-exclusion chromatography of human serum apolipoproteins using an analytical liquid chromatograph
Apolipoproteins, extracted from human serum high-density lipoproteins, can be resolved and recovered with high yield from a preparative MicroPak TSK Type 3000SW size-exclusion column using Tris-buffered 6 m urea or 6 m guanidinium chloride mobile phases. Adequate resolution of some apolipoprotein pa...
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| Veröffentlicht in: | Analytical biochemistry 1982-09, Vol.125 (2), p.386-394 |
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| container_end_page | 394 |
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| container_title | Analytical biochemistry |
| container_volume | 125 |
| creator | Wehr, C.Timothy Cunico, Robert L. Ott, Gary S. Shore, Virgie G. |
| description | Apolipoproteins, extracted from human serum high-density lipoproteins, can be resolved and recovered with high yield from a preparative MicroPak TSK Type 3000SW size-exclusion column using Tris-buffered 6
m urea or 6
m guanidinium chloride mobile phases. Adequate resolution of some apolipoprotein pairs is only achieved at low flow velocities and low sample loads, necessitating repetitive injections of small amounts of material for preparative isolation. An analytical high-performance liquid chromatograph equipped with a simplified sample introduction scheme and low-pressure switching valves for fraction collection was used to isolate milligram quantities of HDL apolipoproteins. |
| doi_str_mv | 10.1016/0003-2697(82)90020-3 |
| format | Article |
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m urea or 6
m guanidinium chloride mobile phases. Adequate resolution of some apolipoprotein pairs is only achieved at low flow velocities and low sample loads, necessitating repetitive injections of small amounts of material for preparative isolation. An analytical high-performance liquid chromatograph equipped with a simplified sample introduction scheme and low-pressure switching valves for fraction collection was used to isolate milligram quantities of HDL apolipoproteins.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/0003-2697(82)90020-3</identifier><identifier>PMID: 7181097</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Apolipoproteins - blood ; Chromatography, High Pressure Liquid - instrumentation ; Humans ; Lipoproteins, HDL - blood ; Lipoproteins, HDL - isolation & purification ; Male</subject><ispartof>Analytical biochemistry, 1982-09, Vol.125 (2), p.386-394</ispartof><rights>1982</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-15963dda5f0cf86fc949d0f79b43b9fe314dd8ef9e3a42658c946682359279d3</citedby><cites>FETCH-LOGICAL-c357t-15963dda5f0cf86fc949d0f79b43b9fe314dd8ef9e3a42658c946682359279d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-2697(82)90020-3$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7181097$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wehr, C.Timothy</creatorcontrib><creatorcontrib>Cunico, Robert L.</creatorcontrib><creatorcontrib>Ott, Gary S.</creatorcontrib><creatorcontrib>Shore, Virgie G.</creatorcontrib><title>Preparative size-exclusion chromatography of human serum apolipoproteins using an analytical liquid chromatograph</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>Apolipoproteins, extracted from human serum high-density lipoproteins, can be resolved and recovered with high yield from a preparative MicroPak TSK Type 3000SW size-exclusion column using Tris-buffered 6
m urea or 6
m guanidinium chloride mobile phases. Adequate resolution of some apolipoprotein pairs is only achieved at low flow velocities and low sample loads, necessitating repetitive injections of small amounts of material for preparative isolation. An analytical high-performance liquid chromatograph equipped with a simplified sample introduction scheme and low-pressure switching valves for fraction collection was used to isolate milligram quantities of HDL apolipoproteins.</description><subject>Apolipoproteins - blood</subject><subject>Chromatography, High Pressure Liquid - instrumentation</subject><subject>Humans</subject><subject>Lipoproteins, HDL - blood</subject><subject>Lipoproteins, HDL - isolation & purification</subject><subject>Male</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu3CAURVHVKpmk_YNWYlU1C7cPsLHZRIqipqkUqV1kjxh4ZKhs4wE7yuTry3RGkbrJisU99wKHkI8MvjJg8hsAiIpL1X7p-IUC4FCJN2TFQMkKBKi3ZPWCnJKznP8AMFY38oSctKwrXLsi298JJ5PMHB6R5vCMFT7ZfskhjtRuUhzMHB-SmTY7Gj3dLIMZaca0DNRMsQ9TnFKcMYyZls74QEtsRtPv5mBNT_uwXYL7f-g9eedNn_HD8Twn9zff769vq7tfP35eX91VVjTtXLFGSeGcaTxY30lvVa0c-Fata7FWHgWrnevQKxSm5rLpCiBlx0WjeKucOCefD7PlgdsF86yHkC32vRkxLll3wIXikhewPoA2xZwTej2lMJi00wz0XrTeW9R7i7rj-p9oLUrt03F_WQ_oXkpHsyW_PORY_vgYMOlsA44WXUhoZ-1ieP2Cv-gRj8Y</recordid><startdate>19820915</startdate><enddate>19820915</enddate><creator>Wehr, C.Timothy</creator><creator>Cunico, Robert L.</creator><creator>Ott, Gary S.</creator><creator>Shore, Virgie G.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19820915</creationdate><title>Preparative size-exclusion chromatography of human serum apolipoproteins using an analytical liquid chromatograph</title><author>Wehr, C.Timothy ; Cunico, Robert L. ; Ott, Gary S. ; Shore, Virgie G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-15963dda5f0cf86fc949d0f79b43b9fe314dd8ef9e3a42658c946682359279d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Apolipoproteins - blood</topic><topic>Chromatography, High Pressure Liquid - instrumentation</topic><topic>Humans</topic><topic>Lipoproteins, HDL - blood</topic><topic>Lipoproteins, HDL - isolation & purification</topic><topic>Male</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wehr, C.Timothy</creatorcontrib><creatorcontrib>Cunico, Robert L.</creatorcontrib><creatorcontrib>Ott, Gary S.</creatorcontrib><creatorcontrib>Shore, Virgie G.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wehr, C.Timothy</au><au>Cunico, Robert L.</au><au>Ott, Gary S.</au><au>Shore, Virgie G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Preparative size-exclusion chromatography of human serum apolipoproteins using an analytical liquid chromatograph</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1982-09-15</date><risdate>1982</risdate><volume>125</volume><issue>2</issue><spage>386</spage><epage>394</epage><pages>386-394</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>Apolipoproteins, extracted from human serum high-density lipoproteins, can be resolved and recovered with high yield from a preparative MicroPak TSK Type 3000SW size-exclusion column using Tris-buffered 6
m urea or 6
m guanidinium chloride mobile phases. Adequate resolution of some apolipoprotein pairs is only achieved at low flow velocities and low sample loads, necessitating repetitive injections of small amounts of material for preparative isolation. An analytical high-performance liquid chromatograph equipped with a simplified sample introduction scheme and low-pressure switching valves for fraction collection was used to isolate milligram quantities of HDL apolipoproteins.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>7181097</pmid><doi>10.1016/0003-2697(82)90020-3</doi><tpages>9</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1982-09, Vol.125 (2), p.386-394 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80239262 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Apolipoproteins - blood Chromatography, High Pressure Liquid - instrumentation Humans Lipoproteins, HDL - blood Lipoproteins, HDL - isolation & purification Male |
| title | Preparative size-exclusion chromatography of human serum apolipoproteins using an analytical liquid chromatograph |
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