Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1
DNAs prepared from whole feline embryo cells infected with a standard laboratory strain of FHV-1, B927, and 50 wild type isolates were digested with a variety of restriction endonucleases. The resulting fragments were separated on agarose gels and Southern blotting performed. To visualize the fragme...
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| Veröffentlicht in: | Japanese journal of veterinary science 1990-10, Vol.52 (5), p.1007-1013 |
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| container_title | Japanese journal of veterinary science |
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| creator | Grail, A. (University of Bristol (United Kingdom)) Harbour, D.A |
| description | DNAs prepared from whole feline embryo cells infected with a standard laboratory strain of FHV-1, B927, and 50 wild type isolates were digested with a variety of restriction endonucleases. The resulting fragments were separated on agarose gels and Southern blotting performed. To visualize the fragments, B927 DNA was purified by pulsed field gel electrophoresis, labelled with alpha 32P and used as a hybridization probe. With most enzymes a large number of the isolates displayed altered mobilities of several fragments with some fuzzy band heterogeneity. A few isolates gave distinct cleavage patterns, in particular using the enzymes Bam HI, Cla I and Sac I. It is suggested that different strains of FHV-1 exist but that changes in the viral genome occur only sporadically and thus may not be readily detected. |
| doi_str_mv | 10.1292/jvms1939.52.1007 |
| format | Article |
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(University of Bristol (United Kingdom))</creatorcontrib><creatorcontrib>Harbour, D.A</creatorcontrib><title>Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1</title><title>Japanese journal of veterinary science</title><addtitle>Nihon Juigaku Zasshi</addtitle><description>DNAs prepared from whole feline embryo cells infected with a standard laboratory strain of FHV-1, B927, and 50 wild type isolates were digested with a variety of restriction endonucleases. The resulting fragments were separated on agarose gels and Southern blotting performed. To visualize the fragments, B927 DNA was purified by pulsed field gel electrophoresis, labelled with alpha 32P and used as a hybridization probe. With most enzymes a large number of the isolates displayed altered mobilities of several fragments with some fuzzy band heterogeneity. A few isolates gave distinct cleavage patterns, in particular using the enzymes Bam HI, Cla I and Sac I. 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(University of Bristol (United Kingdom))</au><au>Harbour, D.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1</atitle><jtitle>Japanese journal of veterinary science</jtitle><addtitle>Nihon Juigaku Zasshi</addtitle><date>1990-10-01</date><risdate>1990</risdate><volume>52</volume><issue>5</issue><spage>1007</spage><epage>1013</epage><pages>1007-1013</pages><issn>0021-5295</issn><eissn>1881-1442</eissn><abstract>DNAs prepared from whole feline embryo cells infected with a standard laboratory strain of FHV-1, B927, and 50 wild type isolates were digested with a variety of restriction endonucleases. The resulting fragments were separated on agarose gels and Southern blotting performed. To visualize the fragments, B927 DNA was purified by pulsed field gel electrophoresis, labelled with alpha 32P and used as a hybridization probe. With most enzymes a large number of the isolates displayed altered mobilities of several fragments with some fuzzy band heterogeneity. A few isolates gave distinct cleavage patterns, in particular using the enzymes Bam HI, Cla I and Sac I. It is suggested that different strains of FHV-1 exist but that changes in the viral genome occur only sporadically and thus may not be readily detected.</abstract><cop>Japan</cop><pmid>2280485</pmid><doi>10.1292/jvms1939.52.1007</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-5295 |
| ispartof | Japanese journal of veterinary science, 1990-10, Vol.52 (5), p.1007-1013 |
| issn | 0021-5295 1881-1442 |
| language | eng |
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| source | MEDLINE; AgriKnowledge（アグリナレッジ）AGROLib |
| subjects | ADN ANALYTICAL METHODS Animals CATS Cats - microbiology Cells, Cultured CHAT CULTIVO DE EMBRIONES CULTURE D'EMBRYON DNA DNA, Viral - isolation & purification EMBRYO CULTURE ENZIMA DE RESTRICCION ENZYME DE RESTRICTION GATO Genes, Viral GENOMAS GENOME GENOMES Herpesviridae - genetics HERPETOVIRIDAE ISOLATION TECHNIQUES NUCLEASAS NUCLEASE NUCLEASES PURIFICACION PURIFICATION RESTRICTION ENZYMES Restriction Mapping TECHNIQUE ANALYTIQUE TECHNIQUE DE L'ISOLEMENT TECNICAS ANALITICAS TECNICAS DE AISLAMIENTO |
| title | Restriction endonuclease analysis of DNA from isolates of feline herpesvirus type 1 |
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