Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies
A micro enzyme-linked immunosorbent assay (ELISA) utilizing a filtration method has been developed which allows the rapid, simple, and sensitive detection of monoclonal antibodies that recognize either soluble or cell surface antigens. This assay involves the immobilization of target cells (or solub...
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| Veröffentlicht in: | Journal of immunological methods 1982-11, Vol.54 (3), p.291-296 |
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| container_end_page | 296 |
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| container_issue | 3 |
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| container_title | Journal of immunological methods |
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| creator | Handley, Harold H. Glassy, Mark C. Cleveland, Patrick H. Royston, Ivor |
| description | A micro enzyme-linked immunosorbent assay (ELISA) utilizing a filtration method has been developed which allows the rapid, simple, and sensitive detection of monoclonal antibodies that recognize either soluble or cell surface antigens. This assay involves the immobilization of target cells (or soluble antigen) onto glass fiber filter discs followed by an incubation with the test hybridoma supernatant and subsequent analysis by ELISA. A specially designed 96-well filtration device is employed which serves both as an incubation chamber and as a filtration manifold. This microELISA requires small volumes of antiserum, few target cells, and can be completed in less than 2 h. This assay is well suited for the rapid screening of murine hybridoma supernatants and can be adapted to detect monoclonal antibodies from other species. |
| doi_str_mv | 10.1016/0022-1759(82)90313-1 |
| format | Article |
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This assay involves the immobilization of target cells (or soluble antigen) onto glass fiber filter discs followed by an incubation with the test hybridoma supernatant and subsequent analysis by ELISA. A specially designed 96-well filtration device is employed which serves both as an incubation chamber and as a filtration manifold. This microELISA requires small volumes of antiserum, few target cells, and can be completed in less than 2 h. 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This assay is well suited for the rapid screening of murine hybridoma supernatants and can be adapted to detect monoclonal antibodies from other species.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - analysis</subject><subject>B-Lymphocytes - immunology</subject><subject>Binding Sites, Antibody</subject><subject>Cell Line</subject><subject>ELISA</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Hybridomas - analysis</subject><subject>Mice</subject><subject>monoclonal antibodies</subject><subject>Polyvinyl Chloride - pharmacology</subject><subject>surface antigens</subject><subject>T-Lymphocytes - immunology</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtu1DAUhi0EKkPLG4DkFSqL0OM4ntgbpKoUqDQSi9K15csJGMV2sJNK8_ZkOqMuWZ3Ffzn6P0LeMfjEgG2vANq2Yb1Ql7L9qIAz3rAXZMNk3za9AvGSbJ4tr8mbWv8AAIMtnJGzrZJcdHxD4hd8xDFPEdNM80ANLWYKnsbgSr7d3d1fU1Or2dMhF1pdQUwh_aK_97YEn6OhdZmwJDObNNcnU1xKSEhjTtmNOZmRrlKw2QesF-TVYMaKb0_3nDx8vf15873Z_fh2d3O9axwX_dxwC70xXkjF1WCFAweohBQInXSWKWFBWul4jyB7Zb3iTngHtuvEOtZ7fk4-HHunkv8uWGcdQ3U4jiZhXqqWKxehZLsau6NxXVtrwUFPJURT9pqBPlDWB4T6gFDLVj9R1myNvT_1Lzaifw6dsK7656OO68jHgEVXFzA59KGgm7XP4f8P_gHAxo1M</recordid><startdate>19821112</startdate><enddate>19821112</enddate><creator>Handley, Harold H.</creator><creator>Glassy, Mark C.</creator><creator>Cleveland, Patrick H.</creator><creator>Royston, Ivor</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19821112</creationdate><title>Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies</title><author>Handley, Harold H. ; Glassy, Mark C. ; Cleveland, Patrick H. ; Royston, Ivor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-3b07aad58939fb5c0c0e9585e048cb195b08b8c37e0879bd93c5dc0b445175dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - analysis</topic><topic>B-Lymphocytes - immunology</topic><topic>Binding Sites, Antibody</topic><topic>Cell Line</topic><topic>ELISA</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Hybridomas - analysis</topic><topic>Mice</topic><topic>monoclonal antibodies</topic><topic>Polyvinyl Chloride - pharmacology</topic><topic>surface antigens</topic><topic>T-Lymphocytes - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Handley, Harold H.</creatorcontrib><creatorcontrib>Glassy, Mark C.</creatorcontrib><creatorcontrib>Cleveland, Patrick H.</creatorcontrib><creatorcontrib>Royston, Ivor</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of immunological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Handley, Harold H.</au><au>Glassy, Mark C.</au><au>Cleveland, Patrick H.</au><au>Royston, Ivor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies</atitle><jtitle>Journal of immunological methods</jtitle><addtitle>J Immunol Methods</addtitle><date>1982-11-12</date><risdate>1982</risdate><volume>54</volume><issue>3</issue><spage>291</spage><epage>296</epage><pages>291-296</pages><issn>0022-1759</issn><eissn>1872-7905</eissn><abstract>A micro enzyme-linked immunosorbent assay (ELISA) utilizing a filtration method has been developed which allows the rapid, simple, and sensitive detection of monoclonal antibodies that recognize either soluble or cell surface antigens. 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| fulltext | fulltext |
| identifier | ISSN: 0022-1759 |
| ispartof | Journal of immunological methods, 1982-11, Vol.54 (3), p.291-296 |
| issn | 0022-1759 1872-7905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80225982 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Antibodies, Monoclonal - analysis B-Lymphocytes - immunology Binding Sites, Antibody Cell Line ELISA Enzyme-Linked Immunosorbent Assay Humans Hybridomas - analysis Mice monoclonal antibodies Polyvinyl Chloride - pharmacology surface antigens T-Lymphocytes - immunology |
| title | Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies |
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