MECHANISM OF ACTION OF A 16-MEMBERED MACROLIDE: CHARACTERISTICS OF DIHYDROROSARAMICIN BINDING TO ESCHERICHIA COLI RIBOSOME AND THE EFFECTS OF SOME COMPETITORS

The macrolide [13H]dihydrorosaramicin binds specifically to 50S and 70S bacterial ribosomal particles. We have studied the influence of salts, pH and additives on the interaction and found that the optimum requirement for salts was 10 mM tris-HCl (pH 7.6), 6 mM MgCl2, 60 mM NH4Cl, and the β-mercapto...

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Veröffentlicht in:	Journal of antibiotics 1982, Vol.35(7), pp.866-874
Hauptverfasser:	SIEGRIST, SYLVIE, VELITCHKOVITCH, SYLVIE, GOFFIC, FRANCOIS LE, MOREAU, NICOLE
Format:	Artikel
Sprache:	eng
Schlagworte:	Anti-Bacterial Agents - pharmacology Binding, Competitive Escherichia coli - metabolism Hydrogen-Ion Concentration Leucomycins - metabolism Mercaptoethanol - pharmacology Ribosomes - metabolism Salts - pharmacology Temperature
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