Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476
Hybrids between a strain of Bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and Corynebacterium acetoacidophilum, a lysine producer, were prepared by protoplast fusion. Based on distinctive parental biochemical characteristics th...
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| Veröffentlicht in: | FEMS microbiology letters 1990-09, Vol.59 (3), p.287-292 |
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| creator | Deb, J K Malik, S Ghosh, V K Mathai, S Sethi, R |
| description | Hybrids between a strain of Bacillus subtilis isolated in our laboratory and having the ability to degrade xylan and other complex polysaccharides and Corynebacterium acetoacidophilum, a lysine producer, were prepared by protoplast fusion. Based on distinctive parental biochemical characteristics the fusants were grouped into 9 categories, viz. BC1 through BC9. Three of the hybrids, BC5, BC7a and BC7b, were tested for their ability to produce xylanase and lysine. Both BC7a and BC7b produced xylanase but BC5 did not, however all of them produced lysine albeit to different degrees. These results demonstrate that intergeneric gene transfer takes place through protoplast fusion between these 2 important genera of bacteria and some of the fusants inherit the useful traits of both the parents. |
| doi_str_mv | 10.1016/0378-1097(90)90235-I |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1990-09, Vol.59 (3), p.287-292 |
| issn | 0378-1097 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80216681 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Alma/SFX Local Collection; Oxford University Press Journals Digital Archive Legacy |
| subjects | Bacillus subtilis - enzymology Cell Fusion Corynebacterium - metabolism Glycoside Hydrolases - biosynthesis Glycoside Hydrolases - genetics Hybrid Cells Lysine - genetics Lysine - metabolism Protoplasts Transfection Xylan Endo-1,3-beta-Xylosidase |
| title | Intergeneric protoplast fusion between xylanase producing Bacillus subtilis LYT and Corynebacterium acetoacidophilum ATCC 21476 |
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