Time-resolved rotational dynamics of phosphorescent-labeled myosin heads in contracting muscle fibers

We have measured the microsecond rotational motions of myosin heads in contracting rabbit psoas muscle fibers by detecting the transient phosphorescence anisotropy of eosin-5-maleimide attached specifically to the myosin head. Experiments were performed on small bundles (10-20 fibers) of glycerinate...

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Veröffentlicht in:	Biochemistry (Easton) 1990-10, Vol.29 (43), p.10023-10031
Hauptverfasser:	Stein, Richard A, Ludescher, Richard D, Dahlberg, Peter S, Fajer, Piotr G, Bennett, Robert L. H, Thomas, David D
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals anisotropy Biological and medical sciences Contractile proteins contraction Eosine Yellowish-(YS) - metabolism Fundamental and applied biological sciences. Psychology heads Holoproteins Luminescent Measurements movement Muscle Contraction muscles myosin Myosins - metabolism phosphorescence Proteins Rabbits rotation Space life sciences
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