Circular dichroism spectroscopy of a cationic porphyrin bound to DNA
Recently, the porphyrin photosensitizer meso-tetra(4-N-methyl-pyridyl)-porphine was identified as a DNA-reactive agent demonstrating both electrostatic and intercalative binding. A series of porphyrin derivatives were synthesized and studied to see if similar compounds manifested identical behavior....
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| Veröffentlicht in: | Biochemical and biophysical research communications 1982-09, Vol.108 (1), p.66-73 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Carvlin, Mark J. Datta-Gupta, Nirmalendu Fiel, Robert J. |
| description | Recently, the porphyrin photosensitizer meso-tetra(4-N-methyl-pyridyl)-porphine was identified as a DNA-reactive agent demonstrating both electrostatic and intercalative binding. A series of porphyrin derivatives were synthesized and studied to see if similar compounds manifested identical behavior. One derivative, meso-tetra(p-N-trimethylanilinium) porphine did not exhibit intercalation behavior but did show avid binding and novel circular dichroic features when bound to B-form DNA. At an ionic strength of 1.02, the binding constant was found to be on the order of 10
4 and higher at lower ionic strength. The large binding constants and induced optical activity suggest that at large porphyrin/DNA ratios the final porphyrin · DNA complex may take the form of a suprahelical helix. |
| doi_str_mv | 10.1016/0006-291X(82)91832-0 |
| format | Article |
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4 and higher at lower ionic strength. The large binding constants and induced optical activity suggest that at large porphyrin/DNA ratios the final porphyrin · DNA complex may take the form of a suprahelical helix.</description><subject>Animals</subject><subject>Cattle</subject><subject>Circular Dichroism</subject><subject>DNA</subject><subject>Kinetics</subject><subject>Mesoporphyrins</subject><subject>Models, Molecular</subject><subject>Nucleic Acid Conformation</subject><subject>Porphyrins</subject><subject>Thymus Gland</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1982</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAQx4Mouj6-gUJOoofqTNJHehGW9QmiFwVvIU2nGNltatIK--3tuotHPc3h_2J-jB0jXCBgfgkAeSJKfDtT4rxEJUUCW2yCUEIiENJtNvm17LH9GD8AENO83GW7BWYgSjVh1zMX7DA3gdfOvgfv4oLHjmwffLS-W3LfcMOt6Z1vneWdD937MriWV35oa957fv00PWQ7jZlHOtrcA_Z6e_Myu08en-8eZtPHxEql-qSpigzG3aJJywqhwlxKQYLKWqIxRSZMakylSKnUUFWZghpRkqTG5pmCjOQBO133dsF_DhR7vXDR0nxuWvJD1AoESpmqf42YZUIoLEZjujba8d8YqNFdcAsTlhpBryjrFUK9QqiV0D-UNYyxk03_UC2o_g1tsI761VqnkcaXo6CjddRaql0Y2erau78HvgGu3Yu-</recordid><startdate>19820916</startdate><enddate>19820916</enddate><creator>Carvlin, Mark J.</creator><creator>Datta-Gupta, Nirmalendu</creator><creator>Fiel, Robert J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19820916</creationdate><title>Circular dichroism spectroscopy of a cationic porphyrin bound to DNA</title><author>Carvlin, Mark J. ; Datta-Gupta, Nirmalendu ; Fiel, Robert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c388t-fb7505027f49b10b16332e2e9d31aa752a4aab8e884aebba7ef29e3efc65805e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1982</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Circular Dichroism</topic><topic>DNA</topic><topic>Kinetics</topic><topic>Mesoporphyrins</topic><topic>Models, Molecular</topic><topic>Nucleic Acid Conformation</topic><topic>Porphyrins</topic><topic>Thymus Gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carvlin, Mark J.</creatorcontrib><creatorcontrib>Datta-Gupta, Nirmalendu</creatorcontrib><creatorcontrib>Fiel, Robert J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carvlin, Mark J.</au><au>Datta-Gupta, Nirmalendu</au><au>Fiel, Robert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Circular dichroism spectroscopy of a cationic porphyrin bound to DNA</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1982-09-16</date><risdate>1982</risdate><volume>108</volume><issue>1</issue><spage>66</spage><epage>73</epage><pages>66-73</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>Recently, the porphyrin photosensitizer meso-tetra(4-N-methyl-pyridyl)-porphine was identified as a DNA-reactive agent demonstrating both electrostatic and intercalative binding. A series of porphyrin derivatives were synthesized and studied to see if similar compounds manifested identical behavior. One derivative, meso-tetra(p-N-trimethylanilinium) porphine did not exhibit intercalation behavior but did show avid binding and novel circular dichroic features when bound to B-form DNA. At an ionic strength of 1.02, the binding constant was found to be on the order of 10
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| ispartof | Biochemical and biophysical research communications, 1982-09, Vol.108 (1), p.66-73 |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cattle Circular Dichroism DNA Kinetics Mesoporphyrins Models, Molecular Nucleic Acid Conformation Porphyrins Thymus Gland |
| title | Circular dichroism spectroscopy of a cationic porphyrin bound to DNA |
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