Genetic recombination in the alpha 2 domain of the E alpha chain yields an Ed molecule with altered T cell activation
We have used a novel T cell selection strategy to isolate a mutant of an H-2d/f murine macrophage line defective in its ability to present antigen to some Ed-restricted helper T cells. This mutant has an amino acid substitution in the alpha 2 domain of the Ed molecule. The mutation changes the seque...
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| Veröffentlicht in: | European journal of immunology 1990-12, Vol.20 (12), p.2571-2576 |
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| container_end_page | 2576 |
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| container_issue | 12 |
| container_start_page | 2571 |
| container_title | European journal of immunology |
| container_volume | 20 |
| creator | Chang, M Y Kowal, C Marzullo, L R Briner, T J Gefter, M L Diamond, B |
| description | We have used a novel T cell selection strategy to isolate a mutant of an H-2d/f murine macrophage line defective in its ability to present antigen to some Ed-restricted helper T cells. This mutant has an amino acid substitution in the alpha 2 domain of the Ed molecule. The mutation changes the sequence at codon 177 from ACC to CAC, which results in a threonine to histidine substitution and appears to be the first in vitro mutation to have arisen by genetic recombination. Even though the mutation is distal to the proposed antigen-binding groove, it affects antigen presentation, presumably by altering the scaffolding for the antigen-binding groove. This type of mutant might not be readily isolated using other selection techniques. |
| doi_str_mv | 10.1002/eji.1830201207 |
| format | Article |
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This mutant has an amino acid substitution in the alpha 2 domain of the Ed molecule. The mutation changes the sequence at codon 177 from ACC to CAC, which results in a threonine to histidine substitution and appears to be the first in vitro mutation to have arisen by genetic recombination. Even though the mutation is distal to the proposed antigen-binding groove, it affects antigen presentation, presumably by altering the scaffolding for the antigen-binding groove. 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| identifier | ISSN: 0014-2980 |
| ispartof | European journal of immunology, 1990-12, Vol.20 (12), p.2571-2576 |
| issn | 0014-2980 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_80205683 |
| source | MEDLINE; Wiley Journals |
| subjects | Amino Acid Sequence Animals Antigen-Presenting Cells - immunology Base Sequence Cell Line DNA - genetics Histocompatibility Antigens Class II - genetics Histocompatibility Antigens Class II - immunology Lymphocyte Activation Mice Molecular Sequence Data Recombination, Genetic T-Lymphocytes, Helper-Inducer - immunology |
| title | Genetic recombination in the alpha 2 domain of the E alpha chain yields an Ed molecule with altered T cell activation |
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