Potentiometric biosensor employing catalytic antibodies as the molecular recognition element
Catalytic antibodies are introduced as an important new class of biomolecules for molecular recognition in biosensors in which the binding sites are continually regenerated by the catalytic reaction of the substrate. Consequently, molecular recognition by catalytic antibodies can yield reversible im...
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| Veröffentlicht in: | Analytical chemistry (Washington) 1990-10, Vol.62 (20), p.2211-2216 |
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| container_end_page | 2216 |
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| container_issue | 20 |
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| container_title | Analytical chemistry (Washington) |
| container_volume | 62 |
| creator | Blackburn, Gary F Talley, David B Booth, Paul M Durfor, Charles N Martin, Mark T Napper, Andrew D Rees, Anthony R |
| description | Catalytic antibodies are introduced as an important new class of biomolecules for molecular recognition in biosensors in which the binding sites are continually regenerated by the catalytic reaction of the substrate. Consequently, molecular recognition by catalytic antibodies can yield reversible immunoblosensors. In this example, a prototype potentiometric biosensor is described in which a micro-pH electrode is modified with a catalytic antibody that catalyzes the hydrolysis of phenyl acetate, producing hydrogen ions that can be monitored by the electrode. The reversible response is linear with the log of substrate concentration over a range of 20-500 microM with a detection limit of 5 microM under the conditions of this study. Alternative applications of catalytic antibodies in other biosensor configurations are discussed. |
| doi_str_mv | 10.1021/ac00219a011 |
| format | Article |
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Alternative applications of catalytic antibodies in other biosensor configurations are discussed.</description><subject>Antibodies, Monoclonal - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydrolysis</subject><subject>Methods. Procedures. 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Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydrolysis</topic><topic>Methods. Procedures. Technologies</topic><topic>Potentiometry - methods</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blackburn, Gary F</creatorcontrib><creatorcontrib>Talley, David B</creatorcontrib><creatorcontrib>Booth, Paul M</creatorcontrib><creatorcontrib>Durfor, Charles N</creatorcontrib><creatorcontrib>Martin, Mark T</creatorcontrib><creatorcontrib>Napper, Andrew D</creatorcontrib><creatorcontrib>Rees, Anthony R</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blackburn, Gary F</au><au>Talley, David B</au><au>Booth, Paul M</au><au>Durfor, Charles N</au><au>Martin, Mark T</au><au>Napper, Andrew D</au><au>Rees, Anthony R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Potentiometric biosensor employing catalytic antibodies as the molecular recognition element</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>1990-10-15</date><risdate>1990</risdate><volume>62</volume><issue>20</issue><spage>2211</spage><epage>2216</epage><pages>2211-2216</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>Catalytic antibodies are introduced as an important new class of biomolecules for molecular recognition in biosensors in which the binding sites are continually regenerated by the catalytic reaction of the substrate. Consequently, molecular recognition by catalytic antibodies can yield reversible immunoblosensors. In this example, a prototype potentiometric biosensor is described in which a micro-pH electrode is modified with a catalytic antibody that catalyzes the hydrolysis of phenyl acetate, producing hydrogen ions that can be monitored by the electrode. The reversible response is linear with the log of substrate concentration over a range of 20-500 microM with a detection limit of 5 microM under the conditions of this study. Alternative applications of catalytic antibodies in other biosensor configurations are discussed.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>2268052</pmid><doi>10.1021/ac00219a011</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 1990-10, Vol.62 (20), p.2211-2216 |
| issn | 0003-2700 1520-6882 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Antibodies, Monoclonal - metabolism Biological and medical sciences Biosensing Techniques Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Hydrolysis Methods. Procedures. Technologies Potentiometry - methods Various methods and equipments |
| title | Potentiometric biosensor employing catalytic antibodies as the molecular recognition element |
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