Rabbit procollagenase synthesized and secreted by a high-yield mammalian expression vector requires stromelysin (matrix metalloproteinase-3) for maximal activation

A chimeric gene composed of the mouse metallothionein promoter linked to the 5' end of the 9.1-kilobase pair rabbit procollagenase (matrix metalloproteinase-1) gene was stably transfected into baby hamster kidney (BHK) cells. Like the native protein, the recombinant procollagenase synthesized a...

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Veröffentlicht in:	The Journal of biological chemistry 1990-12, Vol.265 (36), p.22262-22269
Hauptverfasser:	Brinckerhoff, C E, Suzuki, K, Mitchell, T I, Oram, F, Coon, C I, Palmiter, R D, Nagase, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cell Line Chimera collagenase Collagenases Enzyme Activation Enzyme Precursors - biosynthesis Enzyme Precursors - genetics Enzyme Precursors - metabolism Genes Genetic Vectors Kinetics Matrix Metalloproteinase 3 Metalloendopeptidases - metabolism Metallothionein - genetics Microbial Collagenase - biosynthesis Microbial Collagenase - genetics Microbial Collagenase - metabolism Molecular Sequence Data Oligonucleotide Probes Plasmids procollagenase Promoter Regions, Genetic Rabbits Recombinant Proteins - biosynthesis Recombinant Proteins - metabolism Transfection
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